CAJ | 학술논문

目的探讨全身性创伤与肿瘤生长的关系.方法选用Wistar大鼠36只,Wistar幼鼠1只;以烫伤作为全身性创伤的致伤方法,将大鼠分为三组,分别为对照组、轻度烫伤组及中度烫伤组,每组12只.幼鼠用于Walker-256细胞株腹水瘤传代保种.三组大鼠用幼鼠腹腔培养的肿瘤细胞建立左侧腹股沟移植瘤模型,10天后轻度烫伤组及中度烫伤组于背部分别致10％和15％烫伤,不同时间采血并于14天后采集组织标本.观察不同时间的皮质醇、IL-1β及TNF-α变化.对肿瘤组织行组化检查,观察血管内皮生长因子(VEGF)和增殖细胞核抗原(PCNA)变化.结果大鼠组织烫伤后,皮质醇、IL-1β及TNF-α均较对照组升高,且呈进行性升高,差异有统计学意义.2周取肿瘤检查,肿瘤大小变化显示对照组(0.593 cm3±0.195 cm3)与轻伤组(0.782 cm3 ±0.344 cm3)肿瘤体积,无统计学差异,中度烫伤组(1.006 cm3±0.838 cm3)肿瘤体积明显大于对照组和轻度烫伤组(P＜0.05).肿瘤组织免疫组化显示对照组、轻度烫伤组及中度烫伤的大鼠肿瘤组织中PCNA的表达分别为58.3％±6.6％、77.1％±4.5％和81.6％±4.6％,以中度烫伤变化为大,差异有统计学意义.同时,轻度及中度烫伤组VEGF阳性例数明显高于对照组,差异有统计学意义(P＜0.05).结论烫伤所致不同程度组织创伤反应可引起机体不同炎症介质的释放以及肿瘤组织周围VEGF和PCNA的增加,从而导致肿瘤组织生长速度的改变,说明全身性创伤与肿瘤的生长存在联系.
목적 탐토전신성창상여종류생장적관계.방법 선용Wistar대서36지,Wistar유서1지;이탕상작위전신성창상적치상방법,장대서분위삼조,분별위대조조、경도탕상조급중도탕상조,매조12지.유서용우Walker-256세포주복수류전대보충.삼조대서용유서복강배양적종류세포건립좌측복고구이식류모형,10천후경도탕상조급중도탕상조우배부분별치10％화15％탕상,불동시간채혈병우14천후채집조직표본.관찰불동시간적피질순、IL-1β급TNF-α변화.대종류조직행조화검사,관찰혈관내피생장인자(VEGF)화증식세포핵항원(PCNA)변화.결과 대서조직탕상후,피질순、IL-1β급TNF-α균교대조조승고,차정진행성승고,차이유통계학의의.2주취종류검사,종류대소변화현시대조조(0.593 cm3±0.195 cm3)여경상조(0.782 cm3 ±0.344 cm3)종류체적,무통계학차이,중도탕상조(1.006 cm3±0.838 cm3)종류체적명현대우대조조화경도탕상조(P＜0.05).종류조직면역조화현시대조조、경도탕상조급중도탕상적대서종류조직중PCNA적표체분별위58.3％±6.6％、77.1％±4.5％화81.6％±4.6％,이중도탕상변화위대,차이유통계학의의.동시,경도급중도탕상조VEGF양성례수명현고우대조조,차이유통계학의의(P＜0.05).결론 탕상소치불동정도조직창상반응가인기궤체불동염증개질적석방이급종류조직주위VEGF화PCNA적증가,종이도치종류조직생장속도적개변,설명전신성창상여종류적생장존재련계.
Objective To explore the relationship between systemic trauma and tumor growth.Methods 36 Wistar rats with systemic trauma caused by scald were randomly divided into three groups as control group,mild-scald group and moderate-scald group.And one Wistar pup was used for ascite tumor cell growth (Walker-256 line) which offered to plant the tumor in each groups.The scald was made in the back 10 days after tumor plant with different degrees (10％ and 15％).Blood samples were collected at different time and tumor tissues were harvested after 14 days.laboratory studies including cortisol,IL-1β and TNF-α by ELISA.Vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were observed in Tumor tissues through histochemistry assay.Results The levels of cortisol,ILl β and TNF-αelevated significantly from control group to mild scald group and moderate scald group.Concerning the tumor growth,the size of tumor in control group and mild scald group were (0.593 ± 0.195)cm3 and (0.782 ± 0.344)cm3 respectively without statistic differences,but the size of moderate scald group was (1.006 ± 0.838) cm3 which had significant difference compared to the first two groups.The histochemical studies showed the expression of PCNA in tumor tissues of control group,mild scald group and moderate scald group were 58.3％ ±6.6％,77.1％ ±4.5％ and 81.6％ ±4.6％ with significant difference.And the expression of VEFG in tumor tissue showed the same results as PCNA expression.Conclusions Different degree of scald may cause different severity of traumatic tissue response which could lead to different release of inflammatory mediators and the increase of VEGF and PCNA in tumor tissue.Furthermore all those changes may influence the tumor growth which suggests that systemic trauma has some relationship with tumor growth.