CAJ | 학술논문

目的探讨卡巴胆碱联合复合膳食纤维对烧伤大鼠肠屏障的保护作用.方法以30％总体表面积Ⅲ度烫伤Wistar大鼠为动物实验模型,随机分为生理盐水组、膳食纤维治疗组、卡巴胆碱治疗组、膳食纤维+卡巴胆碱治疗组.分别在伤后6、12、24h活杀动物,另设正常对照组.取小肠在光镜下观察其病理形态变化,取门静脉血检测血浆微量内毒素,取腹主动脉血检测血浆D乳酸的变化.结果 (1)各烫伤组大鼠肠黏膜结构受损程度不同,24h黏膜损伤最为明显.(2)复合膳食纤维联合卡巴胆碱组较其他各组血浆内毒素水平显著降低[24h复合膳食纤维联合卡巴胆碱组为(0.517±0.034) EU/L、生理盐水组为(0.659±0.074) EU/L、膳食纤维治疗组为(0.589±0.027) EU/L、卡巴胆碱治疗组为(0.587±0.026) EU/L,差异均有统计学意义(P均＜0.05).(3)复合膳食纤维联合卡巴胆碱组较其他各组D乳酸水平显著降低[24h复合膳食纤维联合卡巴胆碱组为(102.996±10.102) μg/L、生理盐水组为(133.538±8.621)μg/L、膳食纤维治疗组为(121.223±10.519) μg/L、卡巴胆碱治疗组为(122.700±11.146) μg/L],差异均有统计学意义(P均＜0.05).结论烧伤休克早期联合应用卡巴胆碱与复合膳食纤维对大鼠肠屏障有明显保护作用,并且治疗效果在一定程度上优于单用卡巴胆碱或膳食纤维.
목적 탐토잡파담감연합복합선식섬유대소상대서장병장적보호작용.방법 이30％총체표면적Ⅲ도탕상Wistar대서위동물실험모형,수궤분위생리염수조、선식섬유치료조、잡파담감치료조、선식섬유+잡파담감치료조.분별재상후6、12、24h활살동물,령설정상대조조.취소장재광경하관찰기병리형태변화,취문정맥혈검측혈장미량내독소,취복주동맥혈검측혈장D유산적변화.결과 (1)각탕상조대서장점막결구수손정도불동,24h점막손상최위명현.(2)복합선식섬유연합잡파담감조교기타각조혈장내독소수평현저강저[24h복합선식섬유연합잡파담감조위(0.517±0.034) EU/L、생리염수조위(0.659±0.074) EU/L、선식섬유치료조위(0.589±0.027) EU/L、잡파담감치료조위(0.587±0.026) EU/L,차이균유통계학의의(P균＜0.05).(3)복합선식섬유연합잡파담감조교기타각조D유산수평현저강저[24h복합선식섬유연합잡파담감조위(102.996±10.102) μg/L、생리염수조위(133.538±8.621)μg/L、선식섬유치료조위(121.223±10.519) μg/L、잡파담감치료조위(122.700±11.146) μg/L],차이균유통계학의의(P균＜0.05).결론 소상휴극조기연합응용잡파담감여복합선식섬유대대서장병장유명현보호작용,병차치료효과재일정정도상우우단용잡파담감혹선식섬유.
Objective To investigate the protective effect of the combined application of carlbachol and compound dietary fiber on the intestinal barrier function in burn rats.Methods The 30％ total body surface area (TBSA) Ⅲ scald Wistar rats were randomly divided into four groups.Rats in group A were fed with physiological saline,in group B were fed with compound dietary fiber,in group C were fed with carlbachol and in group D were fed with carlbachol and compound dietary fiber.The rats were sacrificed at 6,12 and 24 hours after treatment,and selected normal rats for control group.Blood samples were taken for detecting plasma endotoxin,D-lactic acid,D-lactic acid content.Tissue samples were harvested from the small intestine for pathological study under light microscopy.Results (1) The damage of the structure of small intestine mucosa was different among 4 groups,and the most obvious was at 24 h after treatment.(2)The level plasma of endotoxin in Group D was (0.517±0.034) EU/L,significant lower than that in group A ((0.659±0.074) EU/L),Group B ((0.589 ±0.027) EU/L) and Group C((0.587±0.026) EU/L),and the difference was significant(P＜0.05).(3) Dlactic acid level in Group D was (102.996±10.102) μg/L,significant lower than that in group A((133.538 ±8.621) μg/L),Group B ((121.223± 10.519) μg/L) and Group C ((122.700± 11.146) μg/L),and the difference was significant(P＜0.05).Conclusion In early stage of burn shock,the therapy of combined with carlbachol and compound dietary fiber has obvious protective effect on rat intestinal barrier.Meanwhile,it is better than the treatment effect of single carlbachol or compound dietary fiber to some extent.