国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2015年
4期
193-195,226
,共4页
猪带绦虫%重组双歧杆菌疫苗%表达%体内%家猪
豬帶縚蟲%重組雙歧桿菌疫苗%錶達%體內%傢豬
저대조충%중조쌍기간균역묘%표체%체내%가저
Taenia solium%Recombinant bifidobacterium vaccine%Expression%In vivo%Domestic pigs
目的 检测3种猪带绦虫重组双歧杆菌候选疫苗在家猪体内的表达情况. 方法 将40日龄健康家猪分别用1011克隆形成单位(colony forming units,CFU)的猪带绦虫Bb-TSO45W-4B、Bb-TSOL18和Bb-TSO45W-4B-TSOL18重组候选疫苗对家猪口服灌胃.首次免疫2周后加强免疫1次.于首免后56 d,剖杀家猪,无菌取肝、肺、脾,采用RT-PCR方法检测上述3种候选疫苗在家猪体内的mRNA表达情况. 结果 在家猪肝脏、肺脏和脾脏中均检测到猪带绦虫TSO45W-4B基因的mRNA,其中肝脏和肺脏的相对表达量为3.3,3.3,1肝脏和肺脏的相对表达量高于脾脏;猪带绦虫TSOL18基因在家猪肝脏和脾脏中均有表达,其中肝脏相对表达量为2.6,高于脾脏的1;猪带绦虫TSO45W-4B-TSOL18融合基因在肝脏、肺脏和脾脏中均有表达,相对表达量依次为0.2、2.4、2.1. 结论 3种猪带绦虫重组双歧杆菌候选疫苗可在家猪体内表达,有利于发挥疫苗的免疫效应.
目的 檢測3種豬帶縚蟲重組雙歧桿菌候選疫苗在傢豬體內的錶達情況. 方法 將40日齡健康傢豬分彆用1011剋隆形成單位(colony forming units,CFU)的豬帶縚蟲Bb-TSO45W-4B、Bb-TSOL18和Bb-TSO45W-4B-TSOL18重組候選疫苗對傢豬口服灌胃.首次免疫2週後加彊免疫1次.于首免後56 d,剖殺傢豬,無菌取肝、肺、脾,採用RT-PCR方法檢測上述3種候選疫苗在傢豬體內的mRNA錶達情況. 結果 在傢豬肝髒、肺髒和脾髒中均檢測到豬帶縚蟲TSO45W-4B基因的mRNA,其中肝髒和肺髒的相對錶達量為3.3,3.3,1肝髒和肺髒的相對錶達量高于脾髒;豬帶縚蟲TSOL18基因在傢豬肝髒和脾髒中均有錶達,其中肝髒相對錶達量為2.6,高于脾髒的1;豬帶縚蟲TSO45W-4B-TSOL18融閤基因在肝髒、肺髒和脾髒中均有錶達,相對錶達量依次為0.2、2.4、2.1. 結論 3種豬帶縚蟲重組雙歧桿菌候選疫苗可在傢豬體內錶達,有利于髮揮疫苗的免疫效應.
목적 검측3충저대조충중조쌍기간균후선역묘재가저체내적표체정황. 방법 장40일령건강가저분별용1011극륭형성단위(colony forming units,CFU)적저대조충Bb-TSO45W-4B、Bb-TSOL18화Bb-TSO45W-4B-TSOL18중조후선역묘대가저구복관위.수차면역2주후가강면역1차.우수면후56 d,부살가저,무균취간、폐、비,채용RT-PCR방법검측상술3충후선역묘재가저체내적mRNA표체정황. 결과 재가저간장、폐장화비장중균검측도저대조충TSO45W-4B기인적mRNA,기중간장화폐장적상대표체량위3.3,3.3,1간장화폐장적상대표체량고우비장;저대조충TSOL18기인재가저간장화비장중균유표체,기중간장상대표체량위2.6,고우비장적1;저대조충TSO45W-4B-TSOL18융합기인재간장、폐장화비장중균유표체,상대표체량의차위0.2、2.4、2.1. 결론 3충저대조충중조쌍기간균후선역묘가재가저체내표체,유리우발휘역묘적면역효응.
Objective To detect the expression of three kinds of recombinant bifidobacterium(Bb) vaccines of Taenia solium in domestic pigs.Methods Healthy domestic pigs of 40 days old were orally administrated with 1011 (colony forming units,CFU) recombinant Bb-TSO45W-4B,Bb-TSOL18 and Bb-TSO45W-4B-TSOL18 vaccines.Two weeks after the first immunization,these domestic pigs were strengthened immunized.56 days after the first immunization,these domestic pigs were killed.The liver,lung and spleen were collected to detect the expression of three kinds of recombinant Bb vaccines of Taenia solium in domestic pigs using reverse transcription-polymerase chain reaction (RT-PCR).Results The TSO45W-4B gene of Taenia solium could be expressed in liver,lung and spleen of domestic pigs.The relative expression was 3.3,3.3,1,respectively.The relative expression in liver and lung was higher than that in spleen;The TSOL18 gene of Taenia solium could be expressed in liver and spleen of domestic pigs.The relative expression was 2.6,1,respectively.The relative expression in liver was higher than that in spleen.The TSO45W-4B-TSOL18 fusion gene of Taenia solium could be expressed in liver,lung and spleen of domestic pigs.The relative expression was 0.2,2.4,2.1,respectively.Conclusion Three kinds of recombinant Bb vaccines of Taenia solium could be expressed in domestic pigs,which would contribute to playing a role in immune effect of vaccines.
