中外医学研究
中外醫學研究
중외의학연구
CHINESE AND FOREIGN MEDICAL RESEARCH
2015年
22期
1-3,4
,共4页
PLLA%珍珠层%骨修复%支架
PLLA%珍珠層%骨脩複%支架
PLLA%진주층%골수복%지가
PLLA%Bone regeneration%Nacreous layer%Scaffold
目的：研制PLLA/珍珠层粉复合支架在修复骨缺损体内降解与生物相容性，为临床应用骨修复提供实验依据。方法：采用冷冻干燥法制备PLLA、PLLA/珍珠层粉支架，用SEM测定其形态表征，用压汞仪测定支架的孔结构。选择24只健康成年新西兰大白兔为实验对象，将选取的24只兔子随机分为四组：A组：PLLA支架材料组，B组：PLLA/珍珠层支架组，C组：微小颗粒骨组；D组：空白组。利用牙钻制成双侧桡骨中段1 cm骨缺损作为骨修复模型，将不同复合支架材料植入缺损处。与术后4、8、12周行影像学、组织学、骨密度检测，并行Lane-sandhu评分，评价复合支架材料的成骨性。结果：成功制备出PLLA/珍珠层粉复合支架，扫描电镜支架断层面呈现出阶梯多孔结构，可见珍珠层粉均匀的分布在孔壁和边缘上，其支架材料的孔隙率在88~93%，双能X射线骨密度仪结合术后X线片得出：术后4、8周B组X线评分和组织学评分略低于C组(P<0.05)，12周两组X线和组织学评分比较差异均无统计学意义(P>0.05)。结论：PLLA/珍珠层支架具有良好的成骨作用，该结果为PLLA/珍珠层支架的临床应用提供了基础。
目的：研製PLLA/珍珠層粉複閤支架在脩複骨缺損體內降解與生物相容性，為臨床應用骨脩複提供實驗依據。方法：採用冷凍榦燥法製備PLLA、PLLA/珍珠層粉支架，用SEM測定其形態錶徵，用壓汞儀測定支架的孔結構。選擇24隻健康成年新西蘭大白兔為實驗對象，將選取的24隻兔子隨機分為四組：A組：PLLA支架材料組，B組：PLLA/珍珠層支架組，C組：微小顆粒骨組；D組：空白組。利用牙鑽製成雙側橈骨中段1 cm骨缺損作為骨脩複模型，將不同複閤支架材料植入缺損處。與術後4、8、12週行影像學、組織學、骨密度檢測，併行Lane-sandhu評分，評價複閤支架材料的成骨性。結果：成功製備齣PLLA/珍珠層粉複閤支架，掃描電鏡支架斷層麵呈現齣階梯多孔結構，可見珍珠層粉均勻的分佈在孔壁和邊緣上，其支架材料的孔隙率在88~93%，雙能X射線骨密度儀結閤術後X線片得齣：術後4、8週B組X線評分和組織學評分略低于C組(P<0.05)，12週兩組X線和組織學評分比較差異均無統計學意義(P>0.05)。結論：PLLA/珍珠層支架具有良好的成骨作用，該結果為PLLA/珍珠層支架的臨床應用提供瞭基礎。
목적：연제PLLA/진주층분복합지가재수복골결손체내강해여생물상용성，위림상응용골수복제공실험의거。방법：채용냉동간조법제비PLLA、PLLA/진주층분지가，용SEM측정기형태표정，용압홍의측정지가적공결구。선택24지건강성년신서란대백토위실험대상，장선취적24지토자수궤분위사조：A조：PLLA지가재료조，B조：PLLA/진주층지가조，C조：미소과립골조；D조：공백조。이용아찬제성쌍측뇨골중단1 cm골결손작위골수복모형，장불동복합지가재료식입결손처。여술후4、8、12주행영상학、조직학、골밀도검측，병행Lane-sandhu평분，평개복합지가재료적성골성。결과：성공제비출PLLA/진주층분복합지가，소묘전경지가단층면정현출계제다공결구，가견진주층분균균적분포재공벽화변연상，기지가재료적공극솔재88~93%，쌍능X사선골밀도의결합술후X선편득출：술후4、8주B조X선평분화조직학평분략저우C조(P<0.05)，12주량조X선화조직학평분비교차이균무통계학의의(P>0.05)。결론：PLLA/진주층지가구유량호적성골작용，해결과위PLLA/진주층지가적림상응용제공료기출。
Objective:To prepare PLLA/pearl layer powder composite scaffolds in repairing bone defects in vivo degradation and biocompatibility, provideing the experimental basis for clinical application of bone repair.Method:Using the freeze drying method to prepare PLLA/pearl layer powder composite scaffolds,its shape characterization was measured by SEM,bracket of pore structure was measured by mercury injection apparatus,scanning method to collect data at low pressure mode.To choose 24 healthy adult New Zealand white rabbit as experimental objects,the selection of the rabbits were randomly divided into four groups:group A(PLLA scaffold materials),group B(PLLA/pearl layer stent group),group C(tiny particles bone group),group D(blank group).Through the establishment of middle 1 cm in bilateral radius as a bone repair bone defect model,to detect different composite materials stents defect.In different cages,for three consecutive days to give gentamicin to prevent infection and after 4,8,12 weeks to go imaging,histology,bone mineral density,graded by Lane-sandhu, and valuated of composite scaffolds into bone.Result:PLLA/pearl layer powder composite scaffold was prepared successfully,scanning were angled trapezium of the porous structure,we could see pearl layer powder evenly distributed on the hole wall and the edge, scaffold porosity basically were all between 88%-93%. Dual-energy X-ray absorptiometry combined postoperative we could see that after 4,8 weeks group B composite support score by X-ray and histological grading were slightly lower than those of group C(P<0.05),after 12 weeks there was no statistical significance between group B and group C(P>0.05).Conclusion:The self-made PLLA/pearl layer powder composite scaffolds has a good osteogenesis effect, and the results are provided the basis for clinical application.
