当代医药论丛
噹代醫藥論叢
당대의약론총
Contemporary Medicine Forum
2015年
14期
42-44
,共3页
ApoA1%ApoB%定标%Logit-log4P%Spline
ApoA1%ApoB%定標%Logit-log4P%Spline
ApoA1%ApoB%정표%Logit-log4P%Spline
ApoA1%ApoB%calibration%Logit-log4P function%Spline funct ion
目的：对比分析在Logit-log4P和Spline定标方式下检测ApoA1、ApoB结果的差异性。方法：分别用Logit-log4P和Spline定标方式检测已知浓度的参考物质，再用其检测38份血清样品中ApoA1的浓度（浓度范围40-220mg/dl）及40份血清样品中ApoB的浓度（浓度范围20-220mg/dl），最后对用两种定标方式进行ApoA1、ApoB检测的结果进行对比分析。结果：采用Logit-log4P和Spline的定标方式进行ApoA1、ApoB检测的结果均具有较高的正确度。用这两种定标方式进行ApoA1检测的结果相比较差异不显著，无统计学意义(P>0.05)。与采用Spline的定标方式相比，采用Logit-log4P的定标方式检测ApoB的指标较低，差异显著，有统计学意义(P<0.05)。根据用这两种定标方式进行ApoA1检测的结果计算其回归方程和相关系数，结果分别为：Yspline =1.100XLogit-log4P-10.10，r=0.997。根据用这两种定标方式进行ApoB检测的结果计算其回归方程和相关系数，结果分别为：Yspline =0.994XLogit-log4P+2.556，r=0.998。结论：采用Logit-log4P和Spline的定标方式均可准确地检测ApoA1、ApoB的指标。在检测低浓度和高浓度ApoA1的血清样本时，用Logit-log4P和Spline的定标方式测定ApoA1的结果有显著的差异性。在检测中间浓度ApoA1的血清样本时，用Logit-log4P和Spline的定标方式测定ApoA1的结果无明显的差异性。在检测低浓度和中间浓度ApoB的血清样本时，用Logit-log4P和Spline的定标方式测定ApoB的结果有显著的差异性。在检测高浓度ApoB的血清样本时，用Logit-log4P和Spline的定标方式测定ApoB的结果无明显的差异性。用这两种定标方式进行ApoA1、ApoB检测的结果具有较好的相关性。
目的：對比分析在Logit-log4P和Spline定標方式下檢測ApoA1、ApoB結果的差異性。方法：分彆用Logit-log4P和Spline定標方式檢測已知濃度的參攷物質，再用其檢測38份血清樣品中ApoA1的濃度（濃度範圍40-220mg/dl）及40份血清樣品中ApoB的濃度（濃度範圍20-220mg/dl），最後對用兩種定標方式進行ApoA1、ApoB檢測的結果進行對比分析。結果：採用Logit-log4P和Spline的定標方式進行ApoA1、ApoB檢測的結果均具有較高的正確度。用這兩種定標方式進行ApoA1檢測的結果相比較差異不顯著，無統計學意義(P>0.05)。與採用Spline的定標方式相比，採用Logit-log4P的定標方式檢測ApoB的指標較低，差異顯著，有統計學意義(P<0.05)。根據用這兩種定標方式進行ApoA1檢測的結果計算其迴歸方程和相關繫數，結果分彆為：Yspline =1.100XLogit-log4P-10.10，r=0.997。根據用這兩種定標方式進行ApoB檢測的結果計算其迴歸方程和相關繫數，結果分彆為：Yspline =0.994XLogit-log4P+2.556，r=0.998。結論：採用Logit-log4P和Spline的定標方式均可準確地檢測ApoA1、ApoB的指標。在檢測低濃度和高濃度ApoA1的血清樣本時，用Logit-log4P和Spline的定標方式測定ApoA1的結果有顯著的差異性。在檢測中間濃度ApoA1的血清樣本時，用Logit-log4P和Spline的定標方式測定ApoA1的結果無明顯的差異性。在檢測低濃度和中間濃度ApoB的血清樣本時，用Logit-log4P和Spline的定標方式測定ApoB的結果有顯著的差異性。在檢測高濃度ApoB的血清樣本時，用Logit-log4P和Spline的定標方式測定ApoB的結果無明顯的差異性。用這兩種定標方式進行ApoA1、ApoB檢測的結果具有較好的相關性。
목적：대비분석재Logit-log4P화Spline정표방식하검측ApoA1、ApoB결과적차이성。방법：분별용Logit-log4P화Spline정표방식검측이지농도적삼고물질，재용기검측38빈혈청양품중ApoA1적농도（농도범위40-220mg/dl）급40빈혈청양품중ApoB적농도（농도범위20-220mg/dl），최후대용량충정표방식진행ApoA1、ApoB검측적결과진행대비분석。결과：채용Logit-log4P화Spline적정표방식진행ApoA1、ApoB검측적결과균구유교고적정학도。용저량충정표방식진행ApoA1검측적결과상비교차이불현저，무통계학의의(P>0.05)。여채용Spline적정표방식상비，채용Logit-log4P적정표방식검측ApoB적지표교저，차이현저，유통계학의의(P<0.05)。근거용저량충정표방식진행ApoA1검측적결과계산기회귀방정화상관계수，결과분별위：Yspline =1.100XLogit-log4P-10.10，r=0.997。근거용저량충정표방식진행ApoB검측적결과계산기회귀방정화상관계수，결과분별위：Yspline =0.994XLogit-log4P+2.556，r=0.998。결론：채용Logit-log4P화Spline적정표방식균가준학지검측ApoA1、ApoB적지표。재검측저농도화고농도ApoA1적혈청양본시，용Logit-log4P화Spline적정표방식측정ApoA1적결과유현저적차이성。재검측중간농도ApoA1적혈청양본시，용Logit-log4P화Spline적정표방식측정ApoA1적결과무명현적차이성。재검측저농도화중간농도ApoB적혈청양본시，용Logit-log4P화Spline적정표방식측정ApoB적결과유현저적차이성。재검측고농도ApoB적혈청양본시，용Logit-log4P화Spline적정표방식측정ApoB적결과무명현적차이성。용저량충정표방식진행ApoA1、ApoB검측적결과구유교호적상관성。
Objective To compare the effects of Logit-log4P and Spline function calibration methods on Determination of ApoA1, ApoB. Methods Determination of Logit-log4P function and Spline function calibration methods for known concentration of reference material , make accuracy analysis of two calibration methods; Determination of ApoA1 of 38 serum samples (range 40-220mg/dl), ApoB of 40 serum samples (range20-220mg/dl), make comparison analysis and correlation analysis between the two calibration methods. Results For ApoA1, samples are divided into 3 groups according to the concentration: 40-100 mg/dl,100-150 mg/dl, 150-220 mg/dl, Logit-log4P calibration results were as follows: 71.86 ± 17.15,121.38 ± 14.11,161.88 ± 21.47, Spline calibration results were as follows: 69.57 ± 16.40,121.56 ± 17.02,170.63 ± 22.92, for each group results ,analysis by t test, the P values were as follows: 0.001,0.845,0.000, the overall analysis of 38 samples by t test, the P value was 0.200. For ApoB, samples were divided into 3 groups according to the concentration: 20-80mg/dl, 80-140mg/dl, 140-220mg/dl, Logit-log4P calibration results were as follows: 47 ± 15.75,105.32 ± 19.84,174.33 ± 23.86, Spline calibration results were as follows: 48.47 ± 16.91,108.05 ± 18.94,175.00 ± 21.76, for each group results ,analysis by t test, the P values were as follows:0.019,0.003,0.666, the overall analysis of 40 samples by t test, the P value was 0.000. Conclusion Two calibration methods have better accuracy. Determination of serum samples on different concentration gradient: ApoA1, at low and high concentrations, there was statistical difference between two methods (P<0.05), at intermediate concentration, two methods showed no statistical difference (P>0.05) ,the overall analysis of 38 samples, two methods showed no statistical difference (P>0.05). ApoB, at low and intermediate concentrations, there was statistical difference between two methods (P<0.05), at high concentrations, two methods showed no statistical difference (P>0.05) , the overall analysis of 40 samples, there was statistical difference between two methods (P<0.05). Results of Logit-log4P were generally lower than that of Spline calibration method.
