目的 观察不同剂量饮水砷暴露后大鼠尿砷代谢形态,血、脑总砷含量及脑组织中总一氧化氮合酶(nitric oxide synthase,NOS)活性变化情况.方法 将5周龄雄性SD大鼠按体质量采用随机数字表法分为4组,每组10只.对照组饮用蒸馏水,5 mg/L亚砷酸钠(NaAsO2)组饮用5 mg/L的NaAsO2水溶液,10 mg/LNaAsO2组饮用10 mg/L的NaAsO2水溶液,50 mg/L NaAsO2组饮用50 mg/L的NaAsO2水溶液.自由饮食、饮水,每周称重1次.连续染毒3个月后处死大鼠,收集血液、尿液、脑组织.采用高效液相色谱-氢化物发生-原子荧光光谱法检测尿中3价无机砷(3 valence inorganic arsenic,iAs3+)、5价无机砷(5 valence inorganic arsenic,iAs5+)、一甲基砷(monomethylated arsenic,MMA)和二甲基砷(dimethylated arsenic,DMA)的含量;氢化物原子荧光光度法检测血、脑总砷含量;分光光度计法检测血、脑组织中总NOS活性.结果 ①体质量:染砷第5～12周,50 mg/LNaAsO2组大鼠体质量[(391.66±32.88)、(410.17±33.47)、(426.96±33.49)、(427.15±32.20)、(441.78±33.69)、(438.27±33.05)、(440.98±33.33)、(441.46±32.45)g]与对照组[(420.93±21.13)、(441.52±28.85)、(462.45±30.57)、(470.16±31.17)、(484.92±32.93)、(483.79±29.63)、(482.02±29.14)、(483.89±29.31)g]比较明显降低(P均＜0.05).②尿砷:对照组,5、10、50 mg/L NaAsO2组iAs3+含量中位数(0.00、57.30、236.33、857.80 μg/L)比较,差异有统计学意义(x2=31.982,P＜0.01);对照组,5、10、50 mg/L NaAsO2组iAs5+含量中位数(0.00、0.00、80.75、162.90μg/L)比较,差异有统计学意义(x2=24.206,P＜0.01);对照组,5、10、50mg/L NaAsO2组DMA含量中位数(12.83、1 711.13、10 386.20、37 038.90μg/L)比较,差异有统计学意义(x2=34.338,P＜ 0.01).③血砷:对照组,5、10、50 mg/L NaAsO2组大鼠血清总砷含量[(5.04±1.57)、(25.40±7.33)、(32.28±7.75)、(56.11±19.87) mg/L]比较,差异有统计学意义(F=27.78,P＜0.05).④脑砷:5、10、50mg/L NaAsO2组脑组织总砷含量[(0.57±0.20)、(1.56±0.52)、(3.63±0.48)mg/kg]与对照组[(0.11±0.06)mg/kg]比较均明显增加(P均＜0.05).⑤NOS活性:与对照组[(27.69±5.56)kU/L]比较,10和50 mg/LNaAsO2组大鼠血清总NOS活性[(33.63±2.26)、(34.19±2.55)kU/L]均明显升高(P均＜0.05);与对照组[(1.79±0.79) U/(mg·prot)]比较,50 mg/L NaAsO2组脑组织总NOS活性[(2.63±0.60)U/(mg·prot)]明显升高(P＜0.05).结论 高砷暴露可增加大鼠血、脑组织中总砷含量及大鼠脑组织总NOS活性.
目的 觀察不同劑量飲水砷暴露後大鼠尿砷代謝形態,血、腦總砷含量及腦組織中總一氧化氮閤酶(nitric oxide synthase,NOS)活性變化情況.方法 將5週齡雄性SD大鼠按體質量採用隨機數字錶法分為4組,每組10隻.對照組飲用蒸餾水,5 mg/L亞砷痠鈉(NaAsO2)組飲用5 mg/L的NaAsO2水溶液,10 mg/LNaAsO2組飲用10 mg/L的NaAsO2水溶液,50 mg/L NaAsO2組飲用50 mg/L的NaAsO2水溶液.自由飲食、飲水,每週稱重1次.連續染毒3箇月後處死大鼠,收集血液、尿液、腦組織.採用高效液相色譜-氫化物髮生-原子熒光光譜法檢測尿中3價無機砷(3 valence inorganic arsenic,iAs3+)、5價無機砷(5 valence inorganic arsenic,iAs5+)、一甲基砷(monomethylated arsenic,MMA)和二甲基砷(dimethylated arsenic,DMA)的含量;氫化物原子熒光光度法檢測血、腦總砷含量;分光光度計法檢測血、腦組織中總NOS活性.結果 ①體質量:染砷第5～12週,50 mg/LNaAsO2組大鼠體質量[(391.66±32.88)、(410.17±33.47)、(426.96±33.49)、(427.15±32.20)、(441.78±33.69)、(438.27±33.05)、(440.98±33.33)、(441.46±32.45)g]與對照組[(420.93±21.13)、(441.52±28.85)、(462.45±30.57)、(470.16±31.17)、(484.92±32.93)、(483.79±29.63)、(482.02±29.14)、(483.89±29.31)g]比較明顯降低(P均＜0.05).②尿砷:對照組,5、10、50 mg/L NaAsO2組iAs3+含量中位數(0.00、57.30、236.33、857.80 μg/L)比較,差異有統計學意義(x2=31.982,P＜0.01);對照組,5、10、50 mg/L NaAsO2組iAs5+含量中位數(0.00、0.00、80.75、162.90μg/L)比較,差異有統計學意義(x2=24.206,P＜0.01);對照組,5、10、50mg/L NaAsO2組DMA含量中位數(12.83、1 711.13、10 386.20、37 038.90μg/L)比較,差異有統計學意義(x2=34.338,P＜ 0.01).③血砷:對照組,5、10、50 mg/L NaAsO2組大鼠血清總砷含量[(5.04±1.57)、(25.40±7.33)、(32.28±7.75)、(56.11±19.87) mg/L]比較,差異有統計學意義(F=27.78,P＜0.05).④腦砷:5、10、50mg/L NaAsO2組腦組織總砷含量[(0.57±0.20)、(1.56±0.52)、(3.63±0.48)mg/kg]與對照組[(0.11±0.06)mg/kg]比較均明顯增加(P均＜0.05).⑤NOS活性:與對照組[(27.69±5.56)kU/L]比較,10和50 mg/LNaAsO2組大鼠血清總NOS活性[(33.63±2.26)、(34.19±2.55)kU/L]均明顯升高(P均＜0.05);與對照組[(1.79±0.79) U/(mg·prot)]比較,50 mg/L NaAsO2組腦組織總NOS活性[(2.63±0.60)U/(mg·prot)]明顯升高(P＜0.05).結論 高砷暴露可增加大鼠血、腦組織中總砷含量及大鼠腦組織總NOS活性.
목적 관찰불동제량음수신폭로후대서뇨신대사형태,혈、뇌총신함량급뇌조직중총일양화담합매(nitric oxide synthase,NOS)활성변화정황.방법 장5주령웅성SD대서안체질량채용수궤수자표법분위4조,매조10지.대조조음용증류수,5 mg/L아신산납(NaAsO2)조음용5 mg/L적NaAsO2수용액,10 mg/LNaAsO2조음용10 mg/L적NaAsO2수용액,50 mg/L NaAsO2조음용50 mg/L적NaAsO2수용액.자유음식、음수,매주칭중1차.련속염독3개월후처사대서,수집혈액、뇨액、뇌조직.채용고효액상색보-경화물발생-원자형광광보법검측뇨중3개무궤신(3 valence inorganic arsenic,iAs3+)、5개무궤신(5 valence inorganic arsenic,iAs5+)、일갑기신(monomethylated arsenic,MMA)화이갑기신(dimethylated arsenic,DMA)적함량;경화물원자형광광도법검측혈、뇌총신함량;분광광도계법검측혈、뇌조직중총NOS활성.결과 ①체질량:염신제5～12주,50 mg/LNaAsO2조대서체질량[(391.66±32.88)、(410.17±33.47)、(426.96±33.49)、(427.15±32.20)、(441.78±33.69)、(438.27±33.05)、(440.98±33.33)、(441.46±32.45)g]여대조조[(420.93±21.13)、(441.52±28.85)、(462.45±30.57)、(470.16±31.17)、(484.92±32.93)、(483.79±29.63)、(482.02±29.14)、(483.89±29.31)g]비교명현강저(P균＜0.05).②뇨신:대조조,5、10、50 mg/L NaAsO2조iAs3+함량중위수(0.00、57.30、236.33、857.80 μg/L)비교,차이유통계학의의(x2=31.982,P＜0.01);대조조,5、10、50 mg/L NaAsO2조iAs5+함량중위수(0.00、0.00、80.75、162.90μg/L)비교,차이유통계학의의(x2=24.206,P＜0.01);대조조,5、10、50mg/L NaAsO2조DMA함량중위수(12.83、1 711.13、10 386.20、37 038.90μg/L)비교,차이유통계학의의(x2=34.338,P＜ 0.01).③혈신:대조조,5、10、50 mg/L NaAsO2조대서혈청총신함량[(5.04±1.57)、(25.40±7.33)、(32.28±7.75)、(56.11±19.87) mg/L]비교,차이유통계학의의(F=27.78,P＜0.05).④뇌신:5、10、50mg/L NaAsO2조뇌조직총신함량[(0.57±0.20)、(1.56±0.52)、(3.63±0.48)mg/kg]여대조조[(0.11±0.06)mg/kg]비교균명현증가(P균＜0.05).⑤NOS활성:여대조조[(27.69±5.56)kU/L]비교,10화50 mg/LNaAsO2조대서혈청총NOS활성[(33.63±2.26)、(34.19±2.55)kU/L]균명현승고(P균＜0.05);여대조조[(1.79±0.79) U/(mg·prot)]비교,50 mg/L NaAsO2조뇌조직총NOS활성[(2.63±0.60)U/(mg·prot)]명현승고(P＜0.05).결론 고신폭로가증가대서혈、뇌조직중총신함량급대서뇌조직총NOS활성.
Objective To observe the changes of the totle nitric oxide synthase (NOS) activity in brain tissue,the metabolism of arsenic speciations in urine and the totle contents in blood,brain after rats drinking water containing different doses of arsenic.Methods Forty SD rats were divided into 4 groups according to random number table,10 rats in each group:control group,5 mg/L NaAsO2 group,10 mg/L NaAsO2 group and 50 mg/L NaAsO2 group.The animals were allowed free access to water and food.Body mass was weighted once a week.Expose to arsenic was continued for three months,then the animals were put to death and their blood,urine and brain tissues were collected.Determination of four kinds of speciations of arsenic (3 valence inorganic arsenic,iAs3+;5 valence inorganic arsenic,iAs5+;monomethylated arsenic,MMA;dimethylated arsenic,DMA) in urine was carried out by high performance liquid chromatography-hydride atomic fluorescence spectrometry.Total arsenic concentration in blood and brain tissue was detected by Atomic Fluorescence Spectrometry.The activity of total NOS in blood and brain tissue was detected using the spectrophotometer method.Results ①Weight:at the 5th-12th week after arsenic exposure,compared with the weight of control group [(420.93 ± 21.13),(441.52 ± 28.85),(462.45 ± 30.57),(470.16 ± 31.17),(484.92 ± 32.93),(483.79 ± 29.63),(482.02 ± 29.14),(483.89 ± 29.31) g],weight of rats in 50 mg/L NaAsO2 group [(391.66 ± 32.88),(410.17 ± 33.47),(426.96 ± 33.49),(427.15 ± 32.20),(441.78 ± 33.69),(438.27 ± 33.05),(440.98 ± 33.33),(441.46 ± 32.45) g] was significantly lighter (all P ＜ 0.05).② Urine arsenic:the medians of iAs3+ content (0.00,57.30,236.33,857.80 μg/L) were compared between control group,5,10 and 50 mg/L NaAsO2 groups,the differences were statistically significant (x2 =31.982,P ＜ 0.01);the medians of iAs5+ content (0.00,0.00,80.75,162.90 μg/L) were compared between control group,5,10 and 50 mg/L NaAsO2 groups,the differences were statistically significant (x2 =24.206,P ＜ 0.01);the medians of DMA content (12.83,1 711.13,l0 386.20,37 038.90 μg/L) were compared between control group,5,10 and 50 mg/L NaAsO2 groups,the differences were statistically significant (x2 =34.338,P ＜ 0.01).③Blood arsenic:total arsenic content in serum of rats [(5.04 ± 1.57),(25.40 ± 7.33),(32.28 ± 7.75),(56.11 ± 19.87) mg/L] was compared between control group,5,10 and 50 mg/L NaAsO2 groups,the differences were statistically significant (F =27.78,P ＜ 0.05).④Brain arsenic:total arsenic content in brain tissue of 5,10 and 50 mg/L NaAsO2 groups [(0.57 ± 0.20),(1.56 ± 0.52),(3.63 ± 0.48) μg/g] was respectively compared with that of control group [(0.11 ± 0.06) μg/g],the differences were statistically significant (all P ＜ 0.05).⑤NOS activity:compared with control group [(27.69 ± 5.56) kU/L],total NOS activity [(33.63 ± 2.26),(34.19 ± 2.55) kU/L] in serum of rats in 10 mg/L NaAsO2 group and 50 mg/L NaAsO2 group increased significantly (all P ＜ 0.05);compared with control group [(1.79 ± 0.79) U/(mg·prot)],total NOS activity [(2.63 ± 0.60)U/(mg ·prot)] in brain tissue of 50 mg/L NaAsO2 group increased significantly (P ＜ 0.05).Conclusions A high dose of arsenic exposure can increase totle contents of arsenic in blood,brain and the activity of total NOS in rat brain tissue.
