中国中西医结合肾病杂志
中國中西醫結閤腎病雜誌
중국중서의결합신병잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN NEPHROLOGY
2015年
7期
583-586
,共4页
平橹%孙艳艳%方敬爱%张晓东
平艣%孫豔豔%方敬愛%張曉東
평로%손염염%방경애%장효동
芡实%糖尿病肾病%基质金属蛋白酶-9%基质金属蛋白酶抑制剂-1%Collagen Ⅳ
芡實%糖尿病腎病%基質金屬蛋白酶-9%基質金屬蛋白酶抑製劑-1%Collagen Ⅳ
검실%당뇨병신병%기질금속단백매-9%기질금속단백매억제제-1%Collagen Ⅳ
Euryale ferox%Diabetic nephropathy%Matrix metalloprotei-nase-9%Tissue inhibitor of metalloproteinases-1%Collagen Ⅳ
目的：：探讨芡实对糖尿病肾病大鼠肾组织MMP-9、TIMP-1及Collagen Ⅳ表达的影响。方法：健康雄性Wistar大鼠65只,随机选取10只为正常对照组( N组)；其余一次性腹腔注射链脲佐菌素( STZ)制作DN模型,造模成功后,随机分为DN组、芡实低、中、高剂量组及氯沙坦钾组,均采用灌胃给药,N组和DN组给予等体积蒸馏水。12周后检测大鼠生化指标；HE、Masson、PAS染色观察肾脏病理变化；免疫组化法测定MMP-9、TIMP-1及Collagen Ⅳ在肾组织表达情况；采用实时荧光定量PCR技术检测肾组织中MMP-9、TIMP-1及Collagen Ⅳ基因的表达。结果：实验12周末,与N组比较,DN组大鼠肾小球肥大,细胞外基质积聚,血糖、24 h尿蛋白定量、血尿素氮( BUN)、肌酐( Scr)、尿白蛋白排泄率( UAER)、尿蛋白/尿肌酐(Up/Ucr)比值显著升高(P<0.05),肾组织MMP-9蛋白和mRNA表达降低(P<0.05),TIMP-1、Collagen Ⅳ蛋白和mRNA表达升高(P<0.05)；与DN组比较,EM组、EH组及LP组大鼠病理改变减轻,24 h尿蛋白定量、Scr、BUN、UAER、Up/Ucr显著降低(P<0.05),肾组织MMP-9蛋白和mRNA表达显著升高(P<0.05),TIMP-1、Collagen Ⅳ蛋白和mRNA表达显著减少(P<0.05)。结论：芡实可能通过调节DN大鼠肾脏MMP-9、TIMP-1的表达,减少细胞外基质积聚,从而减轻肾小球硬化,减少蛋白尿,保护肾功能,延缓DN病变的进展。
目的：：探討芡實對糖尿病腎病大鼠腎組織MMP-9、TIMP-1及Collagen Ⅳ錶達的影響。方法：健康雄性Wistar大鼠65隻,隨機選取10隻為正常對照組( N組)；其餘一次性腹腔註射鏈脲佐菌素( STZ)製作DN模型,造模成功後,隨機分為DN組、芡實低、中、高劑量組及氯沙坦鉀組,均採用灌胃給藥,N組和DN組給予等體積蒸餾水。12週後檢測大鼠生化指標；HE、Masson、PAS染色觀察腎髒病理變化；免疫組化法測定MMP-9、TIMP-1及Collagen Ⅳ在腎組織錶達情況；採用實時熒光定量PCR技術檢測腎組織中MMP-9、TIMP-1及Collagen Ⅳ基因的錶達。結果：實驗12週末,與N組比較,DN組大鼠腎小毬肥大,細胞外基質積聚,血糖、24 h尿蛋白定量、血尿素氮( BUN)、肌酐( Scr)、尿白蛋白排洩率( UAER)、尿蛋白/尿肌酐(Up/Ucr)比值顯著升高(P<0.05),腎組織MMP-9蛋白和mRNA錶達降低(P<0.05),TIMP-1、Collagen Ⅳ蛋白和mRNA錶達升高(P<0.05)；與DN組比較,EM組、EH組及LP組大鼠病理改變減輕,24 h尿蛋白定量、Scr、BUN、UAER、Up/Ucr顯著降低(P<0.05),腎組織MMP-9蛋白和mRNA錶達顯著升高(P<0.05),TIMP-1、Collagen Ⅳ蛋白和mRNA錶達顯著減少(P<0.05)。結論：芡實可能通過調節DN大鼠腎髒MMP-9、TIMP-1的錶達,減少細胞外基質積聚,從而減輕腎小毬硬化,減少蛋白尿,保護腎功能,延緩DN病變的進展。
목적：：탐토검실대당뇨병신병대서신조직MMP-9、TIMP-1급Collagen Ⅳ표체적영향。방법：건강웅성Wistar대서65지,수궤선취10지위정상대조조( N조)；기여일차성복강주사련뇨좌균소( STZ)제작DN모형,조모성공후,수궤분위DN조、검실저、중、고제량조급록사탄갑조,균채용관위급약,N조화DN조급여등체적증류수。12주후검측대서생화지표；HE、Masson、PAS염색관찰신장병리변화；면역조화법측정MMP-9、TIMP-1급Collagen Ⅳ재신조직표체정황；채용실시형광정량PCR기술검측신조직중MMP-9、TIMP-1급Collagen Ⅳ기인적표체。결과：실험12주말,여N조비교,DN조대서신소구비대,세포외기질적취,혈당、24 h뇨단백정량、혈뇨소담( BUN)、기항( Scr)、뇨백단백배설솔( UAER)、뇨단백/뇨기항(Up/Ucr)비치현저승고(P<0.05),신조직MMP-9단백화mRNA표체강저(P<0.05),TIMP-1、Collagen Ⅳ단백화mRNA표체승고(P<0.05)；여DN조비교,EM조、EH조급LP조대서병리개변감경,24 h뇨단백정량、Scr、BUN、UAER、Up/Ucr현저강저(P<0.05),신조직MMP-9단백화mRNA표체현저승고(P<0.05),TIMP-1、Collagen Ⅳ단백화mRNA표체현저감소(P<0.05)。결론：검실가능통과조절DN대서신장MMP-9、TIMP-1적표체,감소세포외기질적취,종이감경신소구경화,감소단백뇨,보호신공능,연완DN병변적진전。
Objective:To investigate the effect of Euryale ferox on the expression of MMP-9, TIMP-1 and CollagenⅣin renal tissues of diabetic ne-phropathy rats. Methods:65 Wistar male rats were used. 10 rats were selected randomly as the normal control group ( N) , the rest of 55 rats were made DN models by injected streptozocin into abdominal cavity ( STZ) , then these rats were randomly divided into 5 groups:diabetic nephropathic model group ( DN) , l-ow-dose Euryale ferox treatment group ( EL) , medium-dose Euryale ferox treatm-ent group ( EM) , high-dose Euryale ferox treatment group ( EH) and losartan pot-assium group ( LP) , and 11 rats in every group, while rats were treated respecti-vely by intragastric administration for 12 weeks with the different dose of Eur-yale ferox and losartan potassium. Meanwhile rats of group N and group DN r-eceived the same volume dis-tilled water by gavage. 24 h urinary protein, serum BUN, Scr, Urinary albumin excretion ratio ( UAER) and the urinary protein to creatinine ratio ( Up/Ucr) were measured after the research. The renal pathologica-l changes were observed by hematoxylin-eosin, Masson and Periodic acid Schif-f (PAS) staining. The expression of MMP-9, TIMP-1 and Collagen Ⅳ were det-ected by im-munohistochemistry. The gene expressions of MMP-9, TIMP-1 and Collagen Ⅳ were measured by Real-time PCR. Results:Compared with group N, the renal glomerular became hypertrophic and the extracellular matrix accum-ulated in group DN. 24 h uri-nary protein, BUN, Scr, UAER and Up/Ucr also in-creased significantly in group DN (P<0. 05);DN group has a decrease of mR-NA of MMP-9 but an increase of the TIMP-1 and Collagen Ⅳ expressions (P<0. 05). While the pathology changes were re-lieved, 24 h urinary protein, BU-N, Scr, UAER and Up/Ucr as well as the mRNA of MMP-9 increased but the decrease of the TIMP-1 and Collagen Ⅳ expressions in group EM, EH and L-P (P<0. 05). Conclusion:Euryale ferox may be affected by the expression of MMP-9 and TIMP-1 in the DN rats kidney, to reduce the extracellular matrix accumulation, thus it may relieve glo-merular sclerosis and decrease proteinuria, meanwhile protect kidney function and delay the progress of diabetic nephropath-y.
