天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
8期
883-885
,共3页
牙周炎%慢性病%牙龈%Wnt蛋白质类%Wnt3a%临床附着丧失
牙週炎%慢性病%牙齦%Wnt蛋白質類%Wnt3a%臨床附著喪失
아주염%만성병%아간%Wnt단백질류%Wnt3a%림상부착상실
periodontitis%chronic disease%gingiva%Wnt proteins%Wnt3a%clinical attachment loss
目的：探讨Wnt3a在慢性牙周炎患者牙龈组织中的表达及其与附着丧失的关系。方法选择15例慢性牙周炎患者（牙周炎组）和15例牙周健康者（对照组），临床检查记录2组的探诊深度和附着丧失水平，采用RT-PCR和Western-Blot检测牙龈组织中Wnt3a的表达水平，并分析Wnt3a蛋白表达与附着丧失的相关性。结果牙周炎组的探诊深度高于对照组（mm：7.93±1.56 vs 1.83±0.37），其临床附着丧失为（7.76±1.34）mm，而对照组的口腔检查中未见临床附着。与对照组比较，牙周炎组的牙龈组织中Wnt3a mRNA（0.49±0.03 vs 0.36±0.04）和蛋白的表达水平（0.57±0.14 vs 0.43±0.17）均较低（t分别为12.015和2.381，P＜0.05）。并且，附着丧失的程度与牙周炎患者牙龈组织中Wnt3a蛋白的表达呈负相关（r=-0.564，P=0.028)。结论 Wnt3a在慢性牙周炎中表达下调，与慢性牙周炎的附着丧失有密切关系，可能在慢性牙周炎的骨吸收中起到了一定的作用。
目的：探討Wnt3a在慢性牙週炎患者牙齦組織中的錶達及其與附著喪失的關繫。方法選擇15例慢性牙週炎患者（牙週炎組）和15例牙週健康者（對照組），臨床檢查記錄2組的探診深度和附著喪失水平，採用RT-PCR和Western-Blot檢測牙齦組織中Wnt3a的錶達水平，併分析Wnt3a蛋白錶達與附著喪失的相關性。結果牙週炎組的探診深度高于對照組（mm：7.93±1.56 vs 1.83±0.37），其臨床附著喪失為（7.76±1.34）mm，而對照組的口腔檢查中未見臨床附著。與對照組比較，牙週炎組的牙齦組織中Wnt3a mRNA（0.49±0.03 vs 0.36±0.04）和蛋白的錶達水平（0.57±0.14 vs 0.43±0.17）均較低（t分彆為12.015和2.381，P＜0.05）。併且，附著喪失的程度與牙週炎患者牙齦組織中Wnt3a蛋白的錶達呈負相關（r=-0.564，P=0.028)。結論 Wnt3a在慢性牙週炎中錶達下調，與慢性牙週炎的附著喪失有密切關繫，可能在慢性牙週炎的骨吸收中起到瞭一定的作用。
목적：탐토Wnt3a재만성아주염환자아간조직중적표체급기여부착상실적관계。방법선택15례만성아주염환자（아주염조）화15례아주건강자（대조조），림상검사기록2조적탐진심도화부착상실수평，채용RT-PCR화Western-Blot검측아간조직중Wnt3a적표체수평，병분석Wnt3a단백표체여부착상실적상관성。결과아주염조적탐진심도고우대조조（mm：7.93±1.56 vs 1.83±0.37），기림상부착상실위（7.76±1.34）mm，이대조조적구강검사중미견림상부착。여대조조비교，아주염조적아간조직중Wnt3a mRNA（0.49±0.03 vs 0.36±0.04）화단백적표체수평（0.57±0.14 vs 0.43±0.17）균교저（t분별위12.015화2.381，P＜0.05）。병차，부착상실적정도여아주염환자아간조직중Wnt3a단백적표체정부상관（r=-0.564，P=0.028)。결론 Wnt3a재만성아주염중표체하조，여만성아주염적부착상실유밀절관계，가능재만성아주염적골흡수중기도료일정적작용。
Objective To study the expression of Wnt3a in chronic periodontitis patients and its clinical significance. Methods Wnt3a expression was examined by RT-PCR and Western-Blot in 15 cases of chronic periodontitis gingival tis?sues and 15 normal tissues. Probe depth and attachment loss were also compared between these two groups. Correlation be?tween Wnt3a expression and attachment lose were also analyzed. Results Probe depth was deeper in chronic periodonititis groups than that in control group(mm:7.93±1.56 vs 1.83±0.37). Attachment loss was(7.76±1.34)mm in chronic periodonti?tis group while it was not seen in control gorup. Wnt3a was lower both in transcription level(0.49±0.03 vs 0.36±0.04)and expression level(0.57±0.14 vs 0.43±0.17, P<0.05) in chronic periodonitis tissue compared with that in normal tissues. The higher clinical attachment loss was negatively correlated with expression of Wnt3a (r=-0.564,P=0.028). Conclusion Wnt3a is expressed less in chronic periodontitis than that in control group. Wnt3a plays an important role in attachment loss and bone absorption.
