CAJ | 학술논문

目的：观察供精体外受精-胚胎移植术（D-IVF）中采用密度梯度离心法联合上游法处理精液后的胚胎情况。方法：回顾本院2013年1月-2014年7月D-IVF 135个周期，观察冷冻精液复苏后经采用密度梯度离心法联合上游法处理后精子总活动率、前向运动精子活动率等精液情况，以及受精以后的受精率、卵裂率、临床妊娠率，对照组为同期46个因丈夫梗阻性无精行ICSI的周期。结果：处理后精子总活动率、前向运动精子活动率均大大提高，但是精子密度却大大下降，处理前后比较差异均有统计学意义（P<0.05）；受精以后的实验室资料和对照组比较，各项参数差异无统计学意义（P>0.05）。结论：密度梯度离心法联合上游法的精液处理方法在D-IVF中是一种稳定可行的方法。
목적：관찰공정체외수정-배태이식술（D-IVF）중채용밀도제도리심법연합상유법처리정액후적배태정황。방법：회고본원2013년1월-2014년7월D-IVF 135개주기，관찰냉동정액복소후경채용밀도제도리심법연합상유법처리후정자총활동솔、전향운동정자활동솔등정액정황，이급수정이후적수정솔、란렬솔、림상임신솔，대조조위동기46개인장부경조성무정행ICSI적주기。결과：처리후정자총활동솔、전향운동정자활동솔균대대제고，단시정자밀도각대대하강，처리전후비교차이균유통계학의의（P<0.05）；수정이후적실험실자료화대조조비교，각항삼수차이무통계학의의（P>0.05）。결론：밀도제도리심법연합상유법적정액처리방법재D-IVF중시일충은정가행적방법。
Objective:To observe embryonic development by using sperm selection prepared by the density gradient centrifugation technique combined with the swim-up method in vitro fertilization and embryo transfer with donor semen(D-IVF). Method:135 cases of routine D-IVF were retrospective analyzed from January 2013 to July 2014. The sperm condense,total activity rate and progressive motility were observed after sperm recovery and sperm selection. The fertilization rate,cleavage rate, high-quality embryo rate and pregnancy rate were compared by the 135 cases and the control groups consist of 46 cases of ICSI (intracytoplasmic sperm injection)with husband semen. Result:After treatment,the total activity rate of sperm and the sperm activity rate were significantly increased,but the sperm density was significantly decreased,the differences were statistically significant(P<0.05).Compared the fertilization rate,cleavage rate,high quality embryo rate and pregnancy rate of two groups, the differences were no statistically significant(P>0.05).Conclusion:The density gradient centrifugation technique combined withthe swim-up method is a stable and feasible method in D-IVF.