中国感染控制杂志
中國感染控製雜誌
중국감염공제잡지
CHINESE JOURNAL OF INFECTION CONTROL
2015年
7期
447-449
,共3页
新生儿%金黄色葡萄球菌%抗药性,微生物%耐消毒剂基因
新生兒%金黃色葡萄毬菌%抗藥性,微生物%耐消毒劑基因
신생인%금황색포도구균%항약성,미생물%내소독제기인
neonate%Staphylococcus aureus%drug resistance,microbial%disinfectant-resistant gene
目的:了解新生儿病房送检标本中金黄色葡萄球菌的耐药谱及耐消毒剂基因携带情况。方法收集2013年1—12月某院新生儿病房住院新生儿送检标本分离的金黄色葡萄球菌,采用VITEK 2 Compact细菌鉴定分析系统进行细菌鉴定及药敏试验,采用聚合酶链反应(PCR)检测耐消毒剂qacA/B基因。结果新生儿科送检标本中共分离225株金黄色葡萄球菌,主要来源于呼吸道(72.44%)和脐部分泌物(14.33%);耐甲氧西林金黄色葡萄球菌(MRSA)检出率为8.89%(20株);耐药监测中,MRSA的耐药率普遍高于甲氧西林敏感金黄色葡萄球菌(MSSA)。左氧氟沙星、莫西沙星、替加环素、万古霉素、利奈唑胺及呋喃妥因对所有金黄色葡萄球菌均显示出良好的抗菌活性,敏感率均为100%。携带qacA/B基因的金黄色葡萄球菌有21株,阳性率为9.33%,其中 MRSA 3株(15.00%), MSSA 18株(8.78%)。结论新生儿病房分离的MRSA耐药严重,同时携带qacA/B基因,应引起临床重视。
目的:瞭解新生兒病房送檢標本中金黃色葡萄毬菌的耐藥譜及耐消毒劑基因攜帶情況。方法收集2013年1—12月某院新生兒病房住院新生兒送檢標本分離的金黃色葡萄毬菌,採用VITEK 2 Compact細菌鑒定分析繫統進行細菌鑒定及藥敏試驗,採用聚閤酶鏈反應(PCR)檢測耐消毒劑qacA/B基因。結果新生兒科送檢標本中共分離225株金黃色葡萄毬菌,主要來源于呼吸道(72.44%)和臍部分泌物(14.33%);耐甲氧西林金黃色葡萄毬菌(MRSA)檢齣率為8.89%(20株);耐藥鑑測中,MRSA的耐藥率普遍高于甲氧西林敏感金黃色葡萄毬菌(MSSA)。左氧氟沙星、莫西沙星、替加環素、萬古黴素、利奈唑胺及呋喃妥因對所有金黃色葡萄毬菌均顯示齣良好的抗菌活性,敏感率均為100%。攜帶qacA/B基因的金黃色葡萄毬菌有21株,暘性率為9.33%,其中 MRSA 3株(15.00%), MSSA 18株(8.78%)。結論新生兒病房分離的MRSA耐藥嚴重,同時攜帶qacA/B基因,應引起臨床重視。
목적:료해신생인병방송검표본중금황색포도구균적내약보급내소독제기인휴대정황。방법수집2013년1—12월모원신생인병방주원신생인송검표본분리적금황색포도구균,채용VITEK 2 Compact세균감정분석계통진행세균감정급약민시험,채용취합매련반응(PCR)검측내소독제qacA/B기인。결과신생인과송검표본중공분리225주금황색포도구균,주요래원우호흡도(72.44%)화제부분비물(14.33%);내갑양서림금황색포도구균(MRSA)검출솔위8.89%(20주);내약감측중,MRSA적내약솔보편고우갑양서림민감금황색포도구균(MSSA)。좌양불사성、막서사성、체가배소、만고매소、리내서알급부남타인대소유금황색포도구균균현시출량호적항균활성,민감솔균위100%。휴대qacA/B기인적금황색포도구균유21주,양성솔위9.33%,기중 MRSA 3주(15.00%), MSSA 18주(8.78%)。결론신생인병방분리적MRSA내약엄중,동시휴대qacA/B기인,응인기림상중시。
Objective To realize drug resistance pattern and disinfectant-resistant genes (qacA/B)of Staphylococcus aureus (S. aureus)from neonates at a neonatal ward.Methods S. aureus from neonates between January and December 2013 were identified and performed antimicrobial susceptibility testing by VITEK 2 Compact system,qacA/B gene was detected by polymerase chain reaction (PCR). Results 225 S. aureus strains were isolated,which were mainly from respiratory tract (72.44% )and umbilical secretion (14.33% );isolation rate of methicillin-resistant S.aureus (MRSA) was 8.89% (n= 20);antimicrobial resistant rate of MRSA was generally higher than methicillin-sensitive S.aureus (MSSA);levofloxacin,moxifloxacin,tigecycline,vancomycin,linezolid,and nitrofurantoin were all had high antimicrobial activity against all S.aureus,susceptibility rates were all 100% . 21(9.33% )S.aureus carried qacA/B,3(15.00% )of which were MRSA,and 18(8.78% )were MSSA.Conclusion Drug resistance of MRSA isolated from neonates in neonatal ward is serious,MRSA strains carry qacA/B,which should be paid more attention.