抑郁症%海马%疾病模型,动物
抑鬱癥%海馬%疾病模型,動物
억욱증%해마%질병모형,동물
Depressive disorder%Hippocampus%Disease models,animal
目的 对抑郁模型大鼠脑血管、海马组织及细胞形态进行动态分析,揭示三者间变化的潜在关系.方法 将30只SD大鼠按体重匹配原则用随机数字表法随机分为对照组及模型组,各15只.对照组不处理,模型组按制备抑郁模型处理.分别于第0、2、4、6、8周对其进行磁共振成像,后摘取海马组织,苏木素-伊红染色,显微镜下观察海马各区细胞形态,同时采用Mimics软件勾画受试大鼠脑血管及海马.分析不同应激时间、脑血管变化与海马组织及细胞形态变化的潜在关系.结果 与对照组相比,随着周龄增加,模型组大鼠大脑中动脉管径及长度逐渐变细缩短,于第8周时最为显著[左侧:对照组(0.31±0.04)、(5.53±0.62)mm,抑郁组(0.25±0.02)、(4.64±0.61) mm,t=5.20、5.48,均P＜0.01;右侧:对照组(0.30±0.03)、(5.59±0.64) mm,抑郁组(0.24±0.03)、(4.72±0.67) mm,t=3.96、4.85,均P＜O.01].与此同时,海马细胞形态也发生显著变化,随着受试大鼠周龄增加,海马DG区、CA1区及CA3区的细胞数目逐渐减少,体积逐渐缩小,核染色加深,排列紊乱、疏松.另外海马体积随着周龄增加亦发生相应变化.与对照组相比,模型组海马体积逐渐缩小,于第8周时最为显著[左侧:对照组(40.62±2.32) mm3,抑郁组(34.18±2.25) mm3,t=7.72,P＜0.01;右侧:对照组(40.47±2.25) mm3,抑郁组(35.63±2.48) mm3,t=5.60,P＜0.01].结论 随着应激时间的增加,抑郁模型大鼠脑血管、海马组织及细胞形态均发生显著变化,且三者间存在一定相关性.
目的 對抑鬱模型大鼠腦血管、海馬組織及細胞形態進行動態分析,揭示三者間變化的潛在關繫.方法 將30隻SD大鼠按體重匹配原則用隨機數字錶法隨機分為對照組及模型組,各15隻.對照組不處理,模型組按製備抑鬱模型處理.分彆于第0、2、4、6、8週對其進行磁共振成像,後摘取海馬組織,囌木素-伊紅染色,顯微鏡下觀察海馬各區細胞形態,同時採用Mimics軟件勾畫受試大鼠腦血管及海馬.分析不同應激時間、腦血管變化與海馬組織及細胞形態變化的潛在關繫.結果 與對照組相比,隨著週齡增加,模型組大鼠大腦中動脈管徑及長度逐漸變細縮短,于第8週時最為顯著[左側:對照組(0.31±0.04)、(5.53±0.62)mm,抑鬱組(0.25±0.02)、(4.64±0.61) mm,t=5.20、5.48,均P＜0.01;右側:對照組(0.30±0.03)、(5.59±0.64) mm,抑鬱組(0.24±0.03)、(4.72±0.67) mm,t=3.96、4.85,均P＜O.01].與此同時,海馬細胞形態也髮生顯著變化,隨著受試大鼠週齡增加,海馬DG區、CA1區及CA3區的細胞數目逐漸減少,體積逐漸縮小,覈染色加深,排列紊亂、疏鬆.另外海馬體積隨著週齡增加亦髮生相應變化.與對照組相比,模型組海馬體積逐漸縮小,于第8週時最為顯著[左側:對照組(40.62±2.32) mm3,抑鬱組(34.18±2.25) mm3,t=7.72,P＜0.01;右側:對照組(40.47±2.25) mm3,抑鬱組(35.63±2.48) mm3,t=5.60,P＜0.01].結論 隨著應激時間的增加,抑鬱模型大鼠腦血管、海馬組織及細胞形態均髮生顯著變化,且三者間存在一定相關性.
목적 대억욱모형대서뇌혈관、해마조직급세포형태진행동태분석,게시삼자간변화적잠재관계.방법 장30지SD대서안체중필배원칙용수궤수자표법수궤분위대조조급모형조,각15지.대조조불처리,모형조안제비억욱모형처리.분별우제0、2、4、6、8주대기진행자공진성상,후적취해마조직,소목소-이홍염색,현미경하관찰해마각구세포형태,동시채용Mimics연건구화수시대서뇌혈관급해마.분석불동응격시간、뇌혈관변화여해마조직급세포형태변화적잠재관계.결과 여대조조상비,수착주령증가,모형조대서대뇌중동맥관경급장도축점변세축단,우제8주시최위현저[좌측:대조조(0.31±0.04)、(5.53±0.62)mm,억욱조(0.25±0.02)、(4.64±0.61) mm,t=5.20、5.48,균P＜0.01;우측:대조조(0.30±0.03)、(5.59±0.64) mm,억욱조(0.24±0.03)、(4.72±0.67) mm,t=3.96、4.85,균P＜O.01].여차동시,해마세포형태야발생현저변화,수착수시대서주령증가,해마DG구、CA1구급CA3구적세포수목축점감소,체적축점축소,핵염색가심,배렬문란、소송.령외해마체적수착주령증가역발생상응변화.여대조조상비,모형조해마체적축점축소,우제8주시최위현저[좌측:대조조(40.62±2.32) mm3,억욱조(34.18±2.25) mm3,t=7.72,P＜0.01;우측:대조조(40.47±2.25) mm3,억욱조(35.63±2.48) mm3,t=5.60,P＜0.01].결론 수착응격시간적증가,억욱모형대서뇌혈관、해마조직급세포형태균발생현저변화,차삼자간존재일정상관성.
Objective To investigate the potential relationship among brain vessels,hippocampus tissue and cell morphology in depressive rat model.Methods According to the weight-atching principle,thirty SD rats were randomly divided into control group (n =15) and depressive group (n =15).Compared with the control group,the depressive group was handled according to the demand of depressive rat model.After rats were scanned by 3.0 magnetic resonance on the 0th,2nd,4th,6th,8th week,respectively,hippocampus was picked out,taken photos and stained by HE.Microscopeat was used to observe hippocampus cell morphology and brain vessels,and hippocampus was described and analysed by Mimics software.Results Diameter and length of the middle cerebral arteries of the model group decreased with time increasing.Compared with the control group,these changes in the model group were significantly different on the 8th week (left:control group (0.31 ± 0.04),(5.53 ± 0.62) mm,depressive group (0.25±0.02),(4.64±0.61) mm,t =5.20,5.48 respectively,both P＜0.01;right:control group (0.30±0.03),(5.59 ±0.64) mm,depressive group (0.24 ±0.03),(4.72 ±0.67) mm,t =3.96,4.85 respectively,both P ＜ 0.01).The cells morphology of hippocampus was also changed with stimulation time increasing.The numbers of cell in DG area,CA1 area and CA3 area were decreased,with cell shrank,cells nuclear stained strongly and arranged loosely.Besides,the volume of hippocampus tissue in the depressive group also changed correspondingly with time.On the 8th week,it was significantly different (left:control group (40.62 ±2.32) mm3,depressive group (34.18 ±2.25) mm3,t =7.72,P＜0.01;right:control group (40.47 ±2.25) mm3,depressive group (35.63 ±2.48) mm3,t =5.60,P ＜0.01).Conclusion With stress time increasing,brain vessels,hippocampus tissue and cell morphology in depressive rats change obviously,indicating certain correlation among them.
