CAJ | 학술논문

为筛选芥蓝×甘蓝型油菜种间杂交后代的遗传背景，加速回交转育进程，采用蕾期授粉结合胚挽救手段进行远缘杂交，获得芥蓝和甘蓝型油菜的种间杂种F1和BC1群体。利用已有的220对SSR引物对双亲进行多态性筛选，获得多态性引物51对。挑选均匀分布在甘蓝9条染色体上、扩增稳定、条带清晰的33对多态性SSR引物，对3株F1单株和35株BC1单株进行遗传背景筛选。NTSYSpc2.11a分析结果表明：F1植株的遗传背景与亲本甘蓝型油菜更为接近，遗传相似系数为0.74；而BC1植株的遗传背景差异较大，与亲本芥蓝的遗传相似系数在0.26～0.65之间。在35株BC1植株中，单株14Y1与芥蓝的遗传背景最为相近，形态观察结果进一步验证了该单株的遗传背景与回交亲本芥蓝更相似，可用于下一步回交转育。
위사선개람×감람형유채충간잡교후대적유전배경，가속회교전육진정，채용뢰기수분결합배만구수단진행원연잡교，획득개람화감람형유채적충간잡충F1화BC1군체。이용이유적220대SSR인물대쌍친진행다태성사선，획득다태성인물51대。도선균균분포재감람9조염색체상、확증은정、조대청석적33대다태성SSR인물，대3주F1단주화35주BC1단주진행유전배경사선。NTSYSpc2.11a분석결과표명：F1식주적유전배경여친본감람형유채경위접근，유전상사계수위0.74；이BC1식주적유전배경차이교대，여친본개람적유전상사계수재0.26～0.65지간。재35주BC1식주중，단주14Y1여개람적유전배경최위상근，형태관찰결과진일보험증료해단주적유전배경여회교친본개람경상사，가용우하일보회교전육。
To screen the genetic background and increase the back-cross breeding efficiency of the interspecific hybrids and their back-cross offsprings between Chinese kale(Brassica oleracea var.alboglabra, CC,2n=2x=18) and rapeseed(Brassica napus,AACC),the F1 inter-specific hybrids and their BC1 backcross offsprings between Ogu-CMS Chinese kale and rapaseed were obtained through hand pollination combined with embryo rescue technology. A total of 220 SSR primers were used to detect polymorphisms between the parents, in which 51 pairs of SSR primers showed polymorphism. 33 pairs of primers were selected to analyze the genetic background of 3 F1 plants and 35 BC1 plants,with even distribution on 9 chromosomes and stable amplifications. The results of analysis by software NTSYSpc2.11a showed that the similarity coefficients between F1 plants and rapeseed was 0.74,which indicated that the genetic background of F1 plants was closer to rapeseed than to Chinese kale,while the similarity coefficients between BC1 plants and Chinese kale varied from 0.26-0.65,with big genetic background differences among BC1 plants.In all 35 BC1 plants,the genetic background of individual 14Y1 was the closest to Chinese kale,which was further confirmed by morphological observation.Thus,the individual 14Y1 could be used as donor plant for further backcross.