CAJ | 학술논문

目的：探讨抑癌基因 p53和 survivin 在肝细胞癌（hepatocellular carcinoma，HCC）中的表达及临床意义。方法采用免疫组化和组织芯片技术检测296例 HCC 中 p53和 survivin 的表达，并采用统计学方法比较其临床意义。结果在296例 HCC 中，p53总的阳性率为13.9%，其阳性表达与 HCC 的组织学分级、术后无瘤生存时间、淋巴结转移和肝脏被膜浸润有关，差异具有统计学意义（P ﹤0.05或0.01），与其他指标无关（P 均﹥0.05）。survivin在 HCC 中总的阳性表达率为72.3%，其阳性表达与 HCC 的组织学分级、临床分期、术后无瘤生存时间、肿瘤直径、淋巴结转移和肝脏被膜浸润有关，差异具有统计学意义（P ﹤0.05或0.01），与其他指标无关（P 均﹥0.05）。Kap-lan-Meier 生存分析显示，p53和 survivin 在 HCC 中阳性表达患者无论其术后无瘤生存时间还是总生存时间均缩短，差异具有统计学意义并（ P 均﹤0.01）。Pearson 相关分析显示，二者在 HCC 中表达呈正相关（ r =0.141，P =0.015）。结论 p53和 survivin 在 HCC 中的表达与肿瘤的临床预后密切相关，两者均可作为 HCC 患者预后不良的判断因子。
목적：탐토억암기인 p53화 survivin 재간세포암（hepatocellular carcinoma，HCC）중적표체급림상의의。방법채용면역조화화조직심편기술검측296례 HCC 중 p53화 survivin 적표체，병채용통계학방법비교기림상의의。결과재296례 HCC 중，p53총적양성솔위13.9%，기양성표체여 HCC 적조직학분급、술후무류생존시간、림파결전이화간장피막침윤유관，차이구유통계학의의（P ﹤0.05혹0.01），여기타지표무관（P 균﹥0.05）。survivin재 HCC 중총적양성표체솔위72.3%，기양성표체여 HCC 적조직학분급、림상분기、술후무류생존시간、종류직경、림파결전이화간장피막침윤유관，차이구유통계학의의（P ﹤0.05혹0.01），여기타지표무관（P 균﹥0.05）。Kap-lan-Meier 생존분석현시，p53화 survivin 재 HCC 중양성표체환자무론기술후무류생존시간환시총생존시간균축단，차이구유통계학의의병（ P 균﹤0.01）。Pearson 상관분석현시，이자재 HCC 중표체정정상관（ r =0.141，P =0.015）。결론 p53화 survivin 재 HCC 중적표체여종류적림상예후밀절상관，량자균가작위 HCC 환자예후불량적판단인자。
Objective:To investigate the expression of p53 and survivin and their clinical significance in HCC. Meth-ods:The expression of p53 and survivin were determined by immunohistochemistry and tissue microarray in 296 cases of HCC and their clinical significance was observed by SPSS. Results:The positive rate of p53 was 13. 9% ,and was closely correlated to the histological grade,the survival months with no tumor after operation,lymph node metastasis and liver cap-sule invasion(all the P ﹤ 0. 05 or 0. 01),but not to the rest of parameters(all the P ﹥ 0. 05). The positive rate of survivin was 72. 3% and was closely correlated to the histological grade,TNM clinical stage,the survival months with no tumor after operation,lymph node metastasis tumor diameter and liver capsule invasion(all the P ﹤ 0. 05 or 0. 01),but not to the rest of parameters(all the P ﹥ 0. 05). The positive expression of p53 and survivin were significantly related to short-term sur-vival by Kaplan-Meier analysis(P ﹤ 0. 01). The two biomarkers were positively interrelated(r = 0. 146,P = 0. 015). Conclusion:The positive expression of p53 and survivin closely correlates with multiple clinicopathological parameters, which may indicate the poor prognosis of HCC.