中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2015年
7期
522-525
,共4页
尿%碘%砷%全自动生化分析仪%方法
尿%碘%砷%全自動生化分析儀%方法
뇨%전%신%전자동생화분석의%방법
Urine%Iodine%Arsenic%Automatic biochemical analyzer%Methods
目的 建立全自动生化分析仪测定尿中碘含量的方法,分析该方法在碘缺乏病监测中的适用性.方法 结合全自动生化分析仪的硬件条件,应用低砷试剂进行尿中碘含量测定,并进行相应的线性范围、检测限、精密度、加标回收、抗干扰和方法对比等验证实验.结果 碘标准曲线线性范围为0～300μg/L,方法检测限为1.57 μg/L(取样量为600μl).精密度:测定尿中碘含量为(84.8±1.1)、(156.5±1.3)、(227.7±2.4) μg/L尿样时,相对标准差(RSD)分别为1.30％、0.83％、1.05％;准确度:对低、中、高3种碘含量尿样加标平均回收率分别为98.0％、100.1％、98.6％,总平均回收率为98.9％.对国家尿碘标准物质进行检测,结果满足准确度的判断标准,此方法准确可靠.方法对比试验,全自动生化分析仪与砷铈催化分光光度法(国标法)各检测24份8～ 10岁儿童尿样,经配对t检验,差异无统计学意义(t=0.35,P＞0.05);与国标法比较,全自动生化分析仪亚砷酸的使用量仅为其2％ (mg/份:0.50比25.00).结论 全自动生化分析仪检测尿碘,操作简单,对环境要求低,检测线性范围合理;使用自配标准溶液和试剂,降低检测的试剂成本;极大降低砷的使用,减少了环境污染和对检测人员健康的危害.该方法实现了自动化检测,检测速度快,适用于碘缺乏病监测中大批量尿碘样品的检测.
目的 建立全自動生化分析儀測定尿中碘含量的方法,分析該方法在碘缺乏病鑑測中的適用性.方法 結閤全自動生化分析儀的硬件條件,應用低砷試劑進行尿中碘含量測定,併進行相應的線性範圍、檢測限、精密度、加標迴收、抗榦擾和方法對比等驗證實驗.結果 碘標準麯線線性範圍為0～300μg/L,方法檢測限為1.57 μg/L(取樣量為600μl).精密度:測定尿中碘含量為(84.8±1.1)、(156.5±1.3)、(227.7±2.4) μg/L尿樣時,相對標準差(RSD)分彆為1.30％、0.83％、1.05％;準確度:對低、中、高3種碘含量尿樣加標平均迴收率分彆為98.0％、100.1％、98.6％,總平均迴收率為98.9％.對國傢尿碘標準物質進行檢測,結果滿足準確度的判斷標準,此方法準確可靠.方法對比試驗,全自動生化分析儀與砷鈰催化分光光度法(國標法)各檢測24份8～ 10歲兒童尿樣,經配對t檢驗,差異無統計學意義(t=0.35,P＞0.05);與國標法比較,全自動生化分析儀亞砷痠的使用量僅為其2％ (mg/份:0.50比25.00).結論 全自動生化分析儀檢測尿碘,操作簡單,對環境要求低,檢測線性範圍閤理;使用自配標準溶液和試劑,降低檢測的試劑成本;極大降低砷的使用,減少瞭環境汙染和對檢測人員健康的危害.該方法實現瞭自動化檢測,檢測速度快,適用于碘缺乏病鑑測中大批量尿碘樣品的檢測.
목적 건립전자동생화분석의측정뇨중전함량적방법,분석해방법재전결핍병감측중적괄용성.방법 결합전자동생화분석의적경건조건,응용저신시제진행뇨중전함량측정,병진행상응적선성범위、검측한、정밀도、가표회수、항간우화방법대비등험증실험.결과 전표준곡선선성범위위0～300μg/L,방법검측한위1.57 μg/L(취양량위600μl).정밀도:측정뇨중전함량위(84.8±1.1)、(156.5±1.3)、(227.7±2.4) μg/L뇨양시,상대표준차(RSD)분별위1.30％、0.83％、1.05％;준학도:대저、중、고3충전함량뇨양가표평균회수솔분별위98.0％、100.1％、98.6％,총평균회수솔위98.9％.대국가뇨전표준물질진행검측,결과만족준학도적판단표준,차방법준학가고.방법대비시험,전자동생화분석의여신시최화분광광도법(국표법)각검측24빈8～ 10세인동뇨양,경배대t검험,차이무통계학의의(t=0.35,P＞0.05);여국표법비교,전자동생화분석의아신산적사용량부위기2％ (mg/빈:0.50비25.00).결론 전자동생화분석의검측뇨전,조작간단,대배경요구저,검측선성범위합리;사용자배표준용액화시제,강저검측적시제성본;겁대강저신적사용,감소료배경오염화대검측인원건강적위해.해방법실현료자동화검측,검측속도쾌,괄용우전결핍병감측중대비량뇨전양품적검측.
Objective To establish a method for determination of urinary iodine by an automatic biochemical analyzer,and to analysis the applicability on the detetion of iodine deficiency disorders.Methods An automatic biochemical analyzer was used to determine urinary iodine content.Linear range,detection limit,precision,recovery and so on were studied.Results The linear range was 0-300 μg/L;the detection limit was 1.57 μg/L (600 μl sample);the relative standard deviations (RSD) were 1.30％,0.83％ and 1.05％ when measuring urine samples with iodine concentrations of (84.8±1.1),(156.5 ± 1.3) and (227.7 ± 2.4)μg/L,respectively;the average recovery was 98.0％,100.1％ and 98.6％,and the total average recovery was 98.9％,when measuring urine samples containing three different iodine concentrations.The test results of standard material were all within the required ranges.The difference of measuring 24 urine samptes in the field by this method and the standard method was not statistically significant by paired t test (t =0.35,P ＞ 0.05).Compared with standard method,the amount usage of arsenic trioxide was reduced to 2％ (0.50 to 25.00 mg per sample).Conclusions The method has theadvantages of simple operation,low requirement for environment,and the linear rang of detection is reasonable.Using this method,the usage of highly toxic reagent can be greatly reduced,as well as the risk of harm to personnel health and the level of environment pollution.The standard solution and reagents can be prepared by ourselves,which greatly reduces the costs.With short reaction time,high detection speed and measuring automatically for large numbers of samples,this method for determination of urinary iodine by an automatic biochemical analyzer can be applied in monitoring of iodine deficiency disorders.
