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SCIENCE AND TECHNOLOGY OF CEREALS,OILS AND FOODS
2015年
4期
93-97
,共5页
白花丹参多糖%复合酶%响应面%提取%抗氧化
白花丹參多糖%複閤酶%響應麵%提取%抗氧化
백화단삼다당%복합매%향응면%제취%항양화
salvia miltiorrhiza f.alba polysaccharide%complex enzyme%response surface methodology%extraction%antioxidation
研究响应面优化复合酶法提取白花丹参多糖的工艺，并评价其抗氧化活性。以白花丹参多糖提取率为响应值，液料比、酶解温度、酶解时间、复合酶（木瓜蛋白酶∶纤维素酶∶果胶酶＝2∶2∶1）添加量为实验因素，采用响应面法建立数学模型，优化提取条件，并初步探讨白花丹参多糖的理化性质，考察白花丹参多糖对 DPPH 和·OH 自由基的清除能力。结果表明，通过二次回归模型响应面分析，液料比、酶解时间、温度、复合酶添加量四因素对白花丹参多糖提取率的影响依次减弱；最佳工艺条件为酶解温度52℃、时间70 min、复合酶添加量8．0 mg／mL、液料比例为45∶1 mL／g，在此条件下多糖提取率为13．36％，模型方程理论预测值为13．70％，两者相对误差小于5％。白花丹参多糖为左旋型、酸性多糖，易溶于水，并具有较强的抗氧化活性，对 DPPH 和·OH 自由基的半数抑制浓度分别为0．969 mg／mL 和3．114 mg／mL，但抗氧化活性小于 VC。采用响应面法优化得到了白花丹参多糖的最佳复合酶提取工艺，得到的多糖具有较强的抗氧化活性。
研究響應麵優化複閤酶法提取白花丹參多糖的工藝，併評價其抗氧化活性。以白花丹參多糖提取率為響應值，液料比、酶解溫度、酶解時間、複閤酶（木瓜蛋白酶∶纖維素酶∶果膠酶＝2∶2∶1）添加量為實驗因素，採用響應麵法建立數學模型，優化提取條件，併初步探討白花丹參多糖的理化性質，攷察白花丹參多糖對 DPPH 和·OH 自由基的清除能力。結果錶明，通過二次迴歸模型響應麵分析，液料比、酶解時間、溫度、複閤酶添加量四因素對白花丹參多糖提取率的影響依次減弱；最佳工藝條件為酶解溫度52℃、時間70 min、複閤酶添加量8．0 mg／mL、液料比例為45∶1 mL／g，在此條件下多糖提取率為13．36％，模型方程理論預測值為13．70％，兩者相對誤差小于5％。白花丹參多糖為左鏇型、痠性多糖，易溶于水，併具有較彊的抗氧化活性，對 DPPH 和·OH 自由基的半數抑製濃度分彆為0．969 mg／mL 和3．114 mg／mL，但抗氧化活性小于 VC。採用響應麵法優化得到瞭白花丹參多糖的最佳複閤酶提取工藝，得到的多糖具有較彊的抗氧化活性。
연구향응면우화복합매법제취백화단삼다당적공예，병평개기항양화활성。이백화단삼다당제취솔위향응치，액료비、매해온도、매해시간、복합매（목과단백매∶섬유소매∶과효매＝2∶2∶1）첨가량위실험인소，채용향응면법건립수학모형，우화제취조건，병초보탐토백화단삼다당적이화성질，고찰백화단삼다당대 DPPH 화·OH 자유기적청제능력。결과표명，통과이차회귀모형향응면분석，액료비、매해시간、온도、복합매첨가량사인소대백화단삼다당제취솔적영향의차감약；최가공예조건위매해온도52℃、시간70 min、복합매첨가량8．0 mg／mL、액료비례위45∶1 mL／g，재차조건하다당제취솔위13．36％，모형방정이론예측치위13．70％，량자상대오차소우5％。백화단삼다당위좌선형、산성다당，역용우수，병구유교강적항양화활성，대 DPPH 화·OH 자유기적반수억제농도분별위0．969 mg／mL 화3．114 mg／mL，단항양화활성소우 VC。채용향응면법우화득도료백화단삼다당적최가복합매제취공예，득도적다당구유교강적항양화활성。
The extraction technology of polysaccharide from salvia miltiorrhiza f.alba by optimized com-plex enzyme method was studied to evaluate its antioxidant activity.Taking polysaccharides extraction rate as response value,the ratio of water to material,enzymolysis temperature and time,and complex en-zyme concentration (papain:cellulase:pectinase =2 ∶2 ∶1 )as experimental factors,the mathematical model was established by response surface method.The extraction conditions were optimized.The physi-co -chemical characteristics of the polysaccharide and the free radical scavenging activity to DPPH and ·OH were analyzed.The result showed that according to quadratic regression model response surface a-nalysis the effect of the 4 factors on polysaccharide yield was decreased in the order of ratio of water to material,extraction time,extraction temperature and complex enzyme concentration.The optimal condi-tions were that the concentration of complex enzyme was 8.0 mg/mL,extraction time was 70 min,the ra-tio of water to material was 45∶1 mL/g,and extraction temperature was 52 ℃.Under the optimal condi-tions,the experimental extraction rate was 13.36%,the predicted extraction rate by mathematical model was 13.70%.The difference between them was less than 5%.The sample was levogyration and acidity polysaccharide,and soluble in water easily with relatively strong antioxidant activity,the IC50 for DPPH and ·OH were 0.969 mg/mL and 3.114 mg/mL,respectively,which was weaker than those of vitamin C.The polysaccharides from salvia miltiorrhiza f.alba extracted by optimal complex enzymatic extraction technology optimized by response surface methodology showed relatively strong antioxidant activity.
