CAJ | 학술논문

目的：探讨胃肠间质瘤的免疫表型及c-kit和血小板源性生长受体α(PDGFRA)基因突变状态。方法应用免疫组化SP法检测110例GIST中Dog-1、CD117、CD34、SMA和S100的表达情况,应用直接测序法检测8例GIST中c-kit及PDGFRA基因的突变状态。结果110例GIST中Dog-1、CD117、CD34、SMA、S100的阳性率分别为92.7%、93.6%、79.1%、44.5%、8.2%。8例GIST中c-kit突变率75%,PDGFRA突变率100%。结论免疫组化可区分大部分GIST病例,c-kit及PDGFRA突变检测对于Dog-1、CD117阴性病例有重要的鉴别诊断价值,并对靶向药物的应用有重要意义。
목적：탐토위장간질류적면역표형급c-kit화혈소판원성생장수체α(PDGFRA)기인돌변상태。방법응용면역조화SP법검측110례GIST중Dog-1、CD117、CD34、SMA화S100적표체정황,응용직접측서법검측8례GIST중c-kit급PDGFRA기인적돌변상태。결과110례GIST중Dog-1、CD117、CD34、SMA、S100적양성솔분별위92.7%、93.6%、79.1%、44.5%、8.2%。8례GIST중c-kit돌변솔75%,PDGFRA돌변솔100%。결론면역조화가구분대부분GIST병례,c-kit급PDGFRA돌변검측대우Dog-1、CD117음성병례유중요적감별진단개치,병대파향약물적응용유중요의의。
Objective To investigate the immunohistochemistry (IHC) features and gene mutation status of c-kit and PDGFRA of GIST. Methods The expression of DOG1, CD117, CD34, SMA and S-100 was detected by immunohistochemical method in 110 cases of GIST. Gene mutation status of c-kit and PDGFRA were detected by direct sequencing in 8 cases of GIST. Results In the patients of 110, the positive rates of DOG1, CD117, CD34, SMA and S-100 were 92.7%, 93.6%, 9.1%, 44.5%and 8.2%respec-tively. Gene mutation rates of c-kit and PDGFRA was 75% and 100% respectively in 8 cases. Conclusion IHC can distinguish most GISTs cases, mutation test of c-kit and PDGFRA is important for differential diagnosis in DOG1 and CD117 negative pa-tients, and have significance to guide the application of targeted drug.