CAJ | 학술논문

目的：口腔扁平苔藓（ OLP）是常见的口腔黏膜病变，部分OLP病例可发生癌变， E-cadherin 是参与细胞间粘附连接的主要分子，在肿瘤的发生、发展及浸润转移中起到重要的作用，成为预示肿瘤进展及预后的重要指标之一。本实验的目的旨在检测E-cadherin在口腔扁平苔癣中的表达，探讨E-cadherin在OLP发病及癌变中的作用，为口腔扁平苔藓癌变的早期诊断和治疗提供依据。方法本研究使用52例口腔扁平苔藓标本蜡块和41例口腔正常黏膜标本蜡块。运用SP免疫组织化学法，以E-cadherin单克隆抗体作为一抗，用已知的E-cadherin阳性的正常口腔黏膜标本作为阳性对照，以PBS代替一抗作为阴性对照，对切片标本进行免疫组化染色，观察阳性染色的分布及强度，对表达情况进行研究分析。结果在52例口腔扁平苔藓标本中25例呈正常阳性表达，27例呈现异常阳性表达，异常阳性率为51?．92％；在41例正常口腔黏膜标本中39例呈正常阳性表达，2例呈现异常阳性表达，异常阳性率为4．88％，2组比较差异有统计学意义。结论 E-cadherin在口腔扁平苔藓标本中的异常阳性表达率明显高于正常口腔黏膜，提示E-cadherin在口腔扁平苔藓恶变的过程中发挥着一定作用，进一步证实口腔扁平苔藓具有潜在恶变的可能。
목적：구강편평태선（ OLP）시상견적구강점막병변，부분OLP병례가발생암변， E-cadherin 시삼여세포간점부련접적주요분자，재종류적발생、발전급침윤전이중기도중요적작용，성위예시종류진전급예후적중요지표지일。본실험적목적지재검측E-cadherin재구강편평태선중적표체，탐토E-cadherin재OLP발병급암변중적작용，위구강편평태선암변적조기진단화치료제공의거。방법본연구사용52례구강편평태선표본사괴화41례구강정상점막표본사괴。운용SP면역조직화학법，이E-cadherin단극륭항체작위일항，용이지적E-cadherin양성적정상구강점막표본작위양성대조，이PBS대체일항작위음성대조，대절편표본진행면역조화염색，관찰양성염색적분포급강도，대표체정황진행연구분석。결과재52례구강편평태선표본중25례정정상양성표체，27례정현이상양성표체，이상양성솔위51?．92％；재41례정상구강점막표본중39례정정상양성표체，2례정현이상양성표체，이상양성솔위4．88％，2조비교차이유통계학의의。결론 E-cadherin재구강편평태선표본중적이상양성표체솔명현고우정상구강점막，제시E-cadherin재구강편평태선악변적과정중발휘착일정작용，진일보증실구강편평태선구유잠재악변적가능。
Objective To investigate the expression of E-cadherin in oral lichen planus ( OLP) and to explore the role of E-cadherin in pathogenesis and carcinomatous change of OLP in order to provide scientific evidences for early diagnosis and treatment of OLP canceration .Methods Fifty-two OLP specimens were collected , at the same time, 41 normal oral mucous membrane specimens were served as control group .Using E-cadherin monoclonal antibody as primary antibody ,taking E-cadherin-positive normal oral mucous membrane specimen as positive controls and using PBS as negative controls instead of primary antibody , these specimens were detected by immmunohistology ( SP ) to observe the distribution and intensity of positive staining, moreover , the expression of E-cadherin was analyzed .Results Twenty-five specimens out of the 52 OLP specimens showed normal positive expression of E-cadherin , however , the other 27 specimens showed abnormal positive expression of E-cadherin, with abnormal positive rate being 51.92%.However in control group, 39 out of 41 specimens showed normal positive expression and 2 specimens showed abnormal positive expression , with abnormal positive rate being 4.88%, there was a significant difference between two groups .Conclusion The abnormal positive expression rate of E-cadherin in OLP is significantly higher than that in normal oral mucous membrane ,which suggests that E-cadherin plays a certain role in the canceration of OLP ,and it is further confirmed that OLP is possible to be cancerated .