CAJ | 학술논문

目的：探讨胃癌组织中Livin、Survivin的表达与化疗药敏性的关系。方法收集102例胃癌标本，用免疫组织化学检测Livin和Survivin的表达。新鲜胃癌组织制备单细胞悬液，分别加入6种化疗药物（奥沙利铂、氟尿嘧啶、顺铂、紫杉醇、阿霉素、环磷酰胺）培养48 h，MTT法观察药物作用后癌细胞活性变化。结果 Livin、Survivin在胃癌组织中表达明显高于组织（ P ＜0*．05）。 Livin在胃癌组织中高表达时，与奥沙利铂、氟尿嘧啶、顺铂耐药性有关（P＜0．05）。 Survivin高表达时，对顺铂、阿霉素、环磷酰胺耐药性有关（ P ＜0．05）。结论肿瘤中Livin、Survivin的过表达与部分化疗药物耐药有关。
목적：탐토위암조직중Livin、Survivin적표체여화료약민성적관계。방법수집102례위암표본，용면역조직화학검측Livin화Survivin적표체。신선위암조직제비단세포현액，분별가입6충화료약물（오사리박、불뇨밀정、순박、자삼순、아매소、배린선알）배양48 h，MTT법관찰약물작용후암세포활성변화。결과 Livin、Survivin재위암조직중표체명현고우조직（ P ＜0*．05）。 Livin재위암조직중고표체시，여오사리박、불뇨밀정、순박내약성유관（P＜0．05）。 Survivin고표체시，대순박、아매소、배린선알내약성유관（ P ＜0．05）。결론종류중Livin、Survivin적과표체여부분화료약물내약유관。
Objective To investigate the correlation between the expression of Livin ,Survivin and chemotherapeutic drug resistance in gastric cancer .Methods One hundred and two specimens of gastric cancer were colleted , and the expression levels of Livin ,Survivin were detected by immunohistochemistry .The single cell suspension was prepared with fresh tumor tissue,then 6 kinds of chemotherapeutic drugs including oxaliplatin , fluorouracil, cisplatin, paclitaxel, adriamycin, cyclophosphamide were added into the suspension which was cultured for 48 hours,finally the changes of cancer cell activity were observed by MTT .Results The expression levels of Livin and Survivin in gastric cancer tissue were significantly higher than those of normal tissues ( P <0.05).The high expression of Livin in gastric cancer was correlated to the drug resistance of oxaliplatin, fluorouracil,cisplatin ( P <0.05).The high expression of Survivin was related with the drug resistance of cisplatin,adriamycin,cyclophosphamide ( P <0.05).Conclusion The overexpression of Livin,the and Survivin is correlated to the part of chemotherapeutic drug resistance in gastric cancer .