蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2015年
7期
851-853
,共3页
肺动脉高压%二十二碳六烯酸%肺动脉平滑肌细胞%细胞凋亡%大鼠
肺動脈高壓%二十二碳六烯痠%肺動脈平滑肌細胞%細胞凋亡%大鼠
폐동맥고압%이십이탄륙희산%폐동맥평활기세포%세포조망%대서
hypoxic pulmonary hypertension%docosahexaenoic acid%pulmonary artery smooth muscle cells%apoptosis%rat
目的：：观察二十二碳六烯酸( DHA)在动物整体水平的抗大鼠肺动脉高压作用及其对肺动脉平滑肌细胞凋亡的影响。方法：将雄性SD大鼠随机分为常氧组、低氧组、低氧DHA处理组，每组7只。大鼠缺氧肺动脉高压模型采用慢性间歇性低氧方法建立，通过右心导管法测定大鼠平均肺动脉压，精确称左右心室质量计算右心室指数，测定肺动脉平滑肌细胞凋亡率和Caspase3、Caspase9活性。结果：与常氧组比较，低氧组大鼠平均肺动脉压和右心室指数均明显升高(P<0.01)，肺动脉平滑肌细胞凋亡率、Caspase3和Caspase9活性均显著下降(P<0.01)；与低氧组比较，低氧DHA处理组大鼠平均肺动脉压和右心室指数均显著下降(P<0.01)，肺动脉平滑肌细胞凋亡率、Caspase3和Caspase9活性均明显升高(P<0.01)。结论：DHA具有抗大鼠肺动脉高压作用，其机制与促进肺动脉平滑肌细胞凋亡有关。
目的：：觀察二十二碳六烯痠( DHA)在動物整體水平的抗大鼠肺動脈高壓作用及其對肺動脈平滑肌細胞凋亡的影響。方法：將雄性SD大鼠隨機分為常氧組、低氧組、低氧DHA處理組，每組7隻。大鼠缺氧肺動脈高壓模型採用慢性間歇性低氧方法建立，通過右心導管法測定大鼠平均肺動脈壓，精確稱左右心室質量計算右心室指數，測定肺動脈平滑肌細胞凋亡率和Caspase3、Caspase9活性。結果：與常氧組比較，低氧組大鼠平均肺動脈壓和右心室指數均明顯升高(P<0.01)，肺動脈平滑肌細胞凋亡率、Caspase3和Caspase9活性均顯著下降(P<0.01)；與低氧組比較，低氧DHA處理組大鼠平均肺動脈壓和右心室指數均顯著下降(P<0.01)，肺動脈平滑肌細胞凋亡率、Caspase3和Caspase9活性均明顯升高(P<0.01)。結論：DHA具有抗大鼠肺動脈高壓作用，其機製與促進肺動脈平滑肌細胞凋亡有關。
목적：：관찰이십이탄륙희산( DHA)재동물정체수평적항대서폐동맥고압작용급기대폐동맥평활기세포조망적영향。방법：장웅성SD대서수궤분위상양조、저양조、저양DHA처리조，매조7지。대서결양폐동맥고압모형채용만성간헐성저양방법건립，통과우심도관법측정대서평균폐동맥압，정학칭좌우심실질량계산우심실지수，측정폐동맥평활기세포조망솔화Caspase3、Caspase9활성。결과：여상양조비교，저양조대서평균폐동맥압화우심실지수균명현승고(P<0.01)，폐동맥평활기세포조망솔、Caspase3화Caspase9활성균현저하강(P<0.01)；여저양조비교，저양DHA처리조대서평균폐동맥압화우심실지수균현저하강(P<0.01)，폐동맥평활기세포조망솔、Caspase3화Caspase9활성균명현승고(P<0.01)。결론：DHA구유항대서폐동맥고압작용，기궤제여촉진폐동맥평활기세포조망유관。
Objective:To observe the role of docosahexaenoic acid( DHA) on anti-pulmonary hypertension in rats and on apoptosis in pulmonary artery smooth muscle cells. Methods:Male Sprague-Dawley(SD) rats(200 to 250g) were randomly divided into control normoxic group,chronic intermittent hypoxic group and DHA-treated group. The hypoxic pulmonary hypertension model was made by chronic intermittent hypoxic method,mean pulmonary arterial pressure was measured by right heart catheterization,the right ventricle index was calculated through weighing the left and right ventricles accurately,the apoptosis rate of pulmonary artery smooth muscle cells was determined by TdT-mediated dVTP nick-end labeling method,Caspase 3 and Caspase 9 activities were measured by activity assay kits. Results:Compared with the control normoxic group,in hypoxia group,mean pulmonary arterial pressure and right ventricular index were significantly increased(P<0. 01),the apoptosis rate of pulmonary artery smooth muscle cells,the activities of Caspase 3 and Caspase 9 were significantly decreased ( P <0. 01 );Compared with the hypoxia group, mean pulmonary arterial pressure and right ventricular index in hypoxia DHA treatment group were decreased(P<0. 01),apoptosis rate of pulmonary artery smooth muscle cells, Caspase 3 and Caspase 9 activities were increased(P<0. 01). Conclusions:DHA has the anti-pulmonary hypertension effect in rats, the mechanism may be associated with promoting apoptosis happening of pulmonary artery smooth muscle cells.
