CAJ | 학술논문

目的：：观察藏茵陈对正常大鼠多耐药相关蛋白3(MRP3)和核受体孕烷X受体(PXR)表达的影响。方法：将10只SD大鼠随机分为藏茵陈组5只，藏茵陈100 mg·kg-1·d-1灌胃7 d；对照组5只，同体积0.9%氯化钠注射液灌胃。7 d后麻醉处死2组大鼠，取肝脏组织行HE染色检测肝脏形态变化，分别提取肝脏组织RNA、膜蛋白及核蛋白，采用实时聚合酶链式反应和蛋白免疫印迹检测膜转运蛋白MRP3和核受体PXR在转录与蛋白水平的表达变化。结果：给予藏茵陈后，与0.9%氯化钠注射液比较，未影响肝脏组织的形态结构；藏茵陈可显著刺激正常大鼠肝细胞膜转运蛋白MRP3表达(P<0.01)；也可明显上调核受体PXR水平增高(P<0.01)。结论：藏茵陈刺激大鼠肝细胞膜蛋白 MRP3的表达上调可能与核受体 PXR途径相关。
목적：：관찰장인진대정상대서다내약상관단백3(MRP3)화핵수체잉완X수체(PXR)표체적영향。방법：장10지SD대서수궤분위장인진조5지，장인진100 mg·kg-1·d-1관위7 d；대조조5지，동체적0.9%록화납주사액관위。7 d후마취처사2조대서，취간장조직행HE염색검측간장형태변화，분별제취간장조직RNA、막단백급핵단백，채용실시취합매련식반응화단백면역인적검측막전운단백MRP3화핵수체PXR재전록여단백수평적표체변화。결과：급여장인진후，여0.9%록화납주사액비교，미영향간장조직적형태결구；장인진가현저자격정상대서간세포막전운단백MRP3표체(P<0.01)；야가명현상조핵수체PXR수평증고(P<0.01)。결론：장인진자격대서간세포막단백 MRP3적표체상조가능여핵수체 PXR도경상관。
Objective:To observe the effects of Artemisiae capillaris on the expressions of multidrug resistance-associated protein 3 (MRP3) and nuclear pregnane X receptor(PXR) in normal rat liver. Methods:Ten male Sprague-Dawley(SD) rats were randomly divided into the Artemisiae capillaris group and control group(5 rats each group). The Artemisiae capillaris group and control group were treated with gavage of Artemisiae capillaris ( 100 mg · kg-1 · d-1 ) and gavage of 0. 9% sodium chloride. Two groups were sacrificed after 7 days. The morphological change of liver tissue was observed using HE staining,the total RNA and membrane or nuclear protein were extracted from the liver tissue of two groups. The mRNA and protein expressions of MRP3 and PXR were detected by Real-time PCR and Western blotting,respectively. Results:Compared with the control group,the morphological structure of liver tissue in Artemisiae capillaris group was not influenced. Artemisiae capillaris could significantly up-regulate the protein expressions of MRP3 and PXR in rat liver(P<0. 01). Conclusions:Artemisiae capillaris can up-regulate the expression of MRP3 and PXR in rat liver.