CAJ | 학술논문

利用平板分离技术，以5种邻苯二甲酸酯类物质（DMP、DEP、DBP、DEHP、DOP）为能源和碳源，对巢湖底泥进行驯化培养，从中筛选出活性菌株 DM1，经鉴定，该菌为皮氏伯克霍尔德氏菌（Burkholderia pickettii）。气相色谱分析的结果表明：B. pickettii.z-1菌对五种混合体系邻苯二甲酸酯的降解趋势符合一级动力学方程：，且随着基质邻苯二甲酸酯浓度梯度的增加，PAEs的降解速率减小。B. pickettii.z-1菌对不同PAEs化合物的降解速率差别很大，较短侧链的 DMP和 DEP降解较快，较长侧链的DEHP、DOP降解较慢。
이용평판분리기술，이5충린분이갑산지류물질（DMP、DEP、DBP、DEHP、DOP）위능원화탄원，대소호저니진행순화배양，종중사선출활성균주 DM1，경감정，해균위피씨백극곽이덕씨균（Burkholderia pickettii）。기상색보분석적결과표명：B. pickettii.z-1균대오충혼합체계린분이갑산지적강해추세부합일급동역학방정：，차수착기질린분이갑산지농도제도적증가，PAEs적강해속솔감소。B. pickettii.z-1균대불동PAEs화합물적강해속솔차별흔대，교단측련적 DMP화 DEP강해교쾌，교장측련적DEHP、DOP강해교만。
By using plate screening techniques with five phthalate esters (DMP, DEP, DBP, DEHP and DOP) as energy and carbon sources, an active strain was isolated from the sediments of Chaohu Lake, which was identified as Burkholderia picketti and named B. picketti .z-1. The biodegradation of five phthalate esters by B. pick-etti .z-1 strain was in accordance with the pseudo first-order kinetic equation: Ct=C0·e-kt. As the concentration of phthalate esters increased, the degradation rate of phthalate esters was reduced. B. picketti .z-1 strain exhibited remarkably different degradation effects on various PAEs. Specifical y, short-side-chain DMP and DEP were degraded rapidly, while long-side-chain DBP and DEHP were degraded slowly.