CAJ | 학술논문

[目的]该研究旨在缩短长白山牛皮杜鹃增殖、生根培养周期。[方法]以长白山牛皮杜鹃组培苗为试材，研究了不同激素组合以及椰乳对牛皮杜鹃组培苗增殖分化的影响，探究了牛皮杜鹃组培苗生根培养基、驯化移栽基质。[结果]改良 MS+IBA 3 mg/L + ZT 1.5 mg/L是牛皮杜鹃组培苗继代增殖的最佳培养基，且在培养基中加入150 ml/L椰乳可明显促进其增值倍数和植株平均高度。牛皮杜鹃组培苗最佳生根培养基为1/4改良 MS+IBA 5.0 mg/L，接种后8 d即开始生根。沙培15 d进行催根处理，适宜基质为草炭土∶腐殖土∶珍珠岩=2∶1∶1，成活率为95.66%。
[목적]해연구지재축단장백산우피두견증식、생근배양주기。[방법]이장백산우피두견조배묘위시재，연구료불동격소조합이급야유대우피두견조배묘증식분화적영향，탐구료우피두견조배묘생근배양기、순화이재기질。[결과]개량 MS+IBA 3 mg/L + ZT 1.5 mg/L시우피두견조배묘계대증식적최가배양기，차재배양기중가입150 ml/L야유가명현촉진기증치배수화식주평균고도。우피두견조배묘최가생근배양기위1/4개량 MS+IBA 5.0 mg/L，접충후8 d즉개시생근。사배15 d진행최근처리，괄의기질위초탄토∶부식토∶진주암=2∶1∶1，성활솔위95.66%。
Objective] This study aimed to shorten the multiplication culture and root-ing culture periods of Rh. chrysanthum Pal . [Method] The Rh. chrysanthum Pal tis-sue culture plantlets col ected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pal tissue culture plantlets were investigated. ln addition, the rooting medium and transplanting matrix for Rh. chrysanthum Pal tissue culture plantlets were explored. [Result] The medium composed of modified MS, lBA (3 mg/L) and ZT (1.5 mg/L) was the optimum medium for subculture mul-tiplication of Rh. chrysanthum Pal tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium (150 mg/L). [Conclusion] For Rh. chrysanthum Pal tissue culture plantlets, the optimum rooting culture medium was composed of modified MS (1/4) and lBA (5.0 mg/L), and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of turfy soil, humus soil and perlite (2:1:1) with a survival rate of 95.66%.