CAJ | 학술논문

以9个高赖氨酸玉米和2个普通玉米杂交种籽粒样品为材料，用日立 L-8900型全自动氨基酸分析仪对高赖氨酸玉米和普通玉米籽粒中赖氨酸含量进行定量测定。了解全自动氨基酸分析仪的检测效果是否可以很好的比较普通玉米与高赖氨酸玉米籽粒中的赖氨酸含量。结果表明高赖氨酸玉米籽粒赖氨酸含量介于0.34%～0.42%之间，普通玉米赖氨酸含量介于0.24%～0.25%，存在显著差异。日立 L-8900型全自动氨基酸分析仪法测定玉米籽粒中赖氨酸含量与其它传统方法相比较，该方法快速、简便、灵敏度高、重现性好、样品需求量少，可以较好的在玉米育种中应用。
이9개고뢰안산옥미화2개보통옥미잡교충자립양품위재료，용일립 L-8900형전자동안기산분석의대고뢰안산옥미화보통옥미자립중뢰안산함량진행정량측정。료해전자동안기산분석의적검측효과시부가이흔호적비교보통옥미여고뢰안산옥미자립중적뢰안산함량。결과표명고뢰안산옥미자립뢰안산함량개우0.34%～0.42%지간，보통옥미뢰안산함량개우0.24%～0.25%，존재현저차이。일립 L-8900형전자동안기산분석의법측정옥미자립중뢰안산함량여기타전통방법상비교，해방법쾌속、간편、령민도고、중현성호、양품수구량소，가이교호적재옥미육충중응용。
ln this study, nine high-lysine maize kernels and two kernels of common maize hybrid were used as experiment materials, and quantitative determination of lysine contents in high-lysine maize kernels and common maize kernels was carried out using Hitachi L-8900 Automatic Amino-acid Analyzer, to know the effect of the Analyzer in distinguishing the lysine contents between common maize kernels and high-lysine maize kernels. The results showed that the lysine contents of high-lysine maize kernels were among 0.34%-0.42%, while of common maize kernels were a-mong 0.24%-0.25%, and the difference was significant. Compared with other tradi-tional methods, this method is rapid, simple, sensitive, highly reproductive and needs fewer maize kernels, thus it is applicable in maize breeding.