郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2015年
4期
466-469
,共4页
许林平%张瑞芳%王鹏%张建营
許林平%張瑞芳%王鵬%張建營
허림평%장서방%왕붕%장건영
肿瘤相关抗原%抗原组合%诊断效能
腫瘤相關抗原%抗原組閤%診斷效能
종류상관항원%항원조합%진단효능
tumor-associated antigen%antigen array%diagnosis value
目的：：评价多种肿瘤相关抗原(TAA)组合在肿瘤诊断中的价值。方法：应用 ELISA 法检测304份不同类型肿瘤(98例肺癌、69例食管癌、50例肝癌、46例结直肠癌、41例乳癌)患者和58份正常人血清中8种 TAA (Koc、p62、IMP1、cyclinE、p16、survivin、c-myc、p53)的自身抗体,用蛋白印迹技术验证其免疫活性,评价用 TAA 诊断肿瘤的真实性、可靠性和收益。结果：肿瘤患者血清中8种 TAA 阳性率普遍偏低,随着 TAA 的增多直至8种 TAA组合,对肿瘤的诊断灵敏度达到63.5%,特异度达86.2%,对不同类型肿瘤诊断的约登指数为0.423~0.522,阳性、阴性预测值为74.2%~88.7%、58.8%~75.8%,阳性、阴性似然比4.07~4.76、0.39~0.51,8种 TAA 诊断肿瘤的一致率和 Kappa 值分别为67.1%和0.51。结论：多种 TAA 组合对肿瘤具有较高的诊断价值。
目的：：評價多種腫瘤相關抗原(TAA)組閤在腫瘤診斷中的價值。方法：應用 ELISA 法檢測304份不同類型腫瘤(98例肺癌、69例食管癌、50例肝癌、46例結直腸癌、41例乳癌)患者和58份正常人血清中8種 TAA (Koc、p62、IMP1、cyclinE、p16、survivin、c-myc、p53)的自身抗體,用蛋白印跡技術驗證其免疫活性,評價用 TAA 診斷腫瘤的真實性、可靠性和收益。結果：腫瘤患者血清中8種 TAA 暘性率普遍偏低,隨著 TAA 的增多直至8種 TAA組閤,對腫瘤的診斷靈敏度達到63.5%,特異度達86.2%,對不同類型腫瘤診斷的約登指數為0.423~0.522,暘性、陰性預測值為74.2%~88.7%、58.8%~75.8%,暘性、陰性似然比4.07~4.76、0.39~0.51,8種 TAA 診斷腫瘤的一緻率和 Kappa 值分彆為67.1%和0.51。結論：多種 TAA 組閤對腫瘤具有較高的診斷價值。
목적：：평개다충종류상관항원(TAA)조합재종류진단중적개치。방법：응용 ELISA 법검측304빈불동류형종류(98례폐암、69례식관암、50례간암、46례결직장암、41례유암)환자화58빈정상인혈청중8충 TAA (Koc、p62、IMP1、cyclinE、p16、survivin、c-myc、p53)적자신항체,용단백인적기술험증기면역활성,평개용 TAA 진단종류적진실성、가고성화수익。결과：종류환자혈청중8충 TAA 양성솔보편편저,수착 TAA 적증다직지8충 TAA조합,대종류적진단령민도체도63.5%,특이도체86.2%,대불동류형종류진단적약등지수위0.423~0.522,양성、음성예측치위74.2%~88.7%、58.8%~75.8%,양성、음성사연비4.07~4.76、0.39~0.51,8충 TAA 진단종류적일치솔화 Kappa 치분별위67.1%화0.51。결론：다충 TAA 조합대종류구유교고적진단개치。
Aim: To evaluate the diagnostic value of multiple tumor-associated antigens(TAAs) in different types of cancer. Methods: Eight TAAs including Koc, p62, IMP1, cyclinE, p16, survivin, c-myc and p53 in sera from 304 canc-er patients (98 cases of lung cancer,69 cases of esophageal cancer,50 cases of liver cancer,46 cases of colorectal cancer and 41 cases of breast cancer) and 58 normal individuals were detected by ELISA. Positive results of ELISA were con-firmed by Western blot. Results: The positive rate of the 8 TAAs in different cancer patients were generally low. With the successive addition of TAA to a final total of eight antigens, there was a stepwise increase of positive reactions reaching to a sensitivity of 63. 5% and a specificity of 86. 2% . In different cancer, the Youden′s index was 0. 423 - 0. 522, positive and negative predictive value was 74. 2% - 88. 7% and 58. 8% - 75. 8% , while positive and negative likelihood ratio was 4. 07 - 4. 76 and 0. 39 - 0. 51, which showed that the clinical diagnostic value of a assays of 8 TAAs was high. Agreement rate and Kappa value were 67. 1% and 0. 51, respectively. Conclusion: Multiple TAA arrays may have higher diagnostic value for tumor diagnosis.
