CAJ | 학술논문

目的：探讨天津滨海地区脑梗死发生与血管紧张素转化酶（ACE）多态性位点 rs4646994和rs35397082基因多态性的相关性。方法采用基因测序及DNA 电泳技术分别检测53例脑梗死患者和53例健康志愿者的 ACE的单核苷酸多态性（SNP）。同时收集各组血清样本，行血清 ACE检测。结果健康对照组和脑梗死患者组，rs35397082均为插入缺失型。对于rs4646994，健康对照组中rs4646994的插入型 SNP有45例（84．91％），缺失型为8例（15．09％）；脑梗死患者组中插入型47例（88．68％），缺失型为6例（11．32％），差异无统计学意义（P＞0．05）。健康对照组血清ACE水平与脑梗死患者组比较差异有统计学意义（P＜0．05），脑梗死患者组低于健康对照组。结论 ACE基因rs4646994和rs35397082多态性与天津滨海地区健康人及急性脑梗死无显著关联，二者血清水平差异不是由这两个位点差异所致，ACE基因的这两种SNP不是天津滨海地区急性脑梗死发生的独立危险因子。
목적：탐토천진빈해지구뇌경사발생여혈관긴장소전화매（ACE）다태성위점 rs4646994화rs35397082기인다태성적상관성。방법채용기인측서급DNA 전영기술분별검측53례뇌경사환자화53례건강지원자적 ACE적단핵감산다태성（SNP）。동시수집각조혈청양본，행혈청 ACE검측。결과건강대조조화뇌경사환자조，rs35397082균위삽입결실형。대우rs4646994，건강대조조중rs4646994적삽입형 SNP유45례（84．91％），결실형위8례（15．09％）；뇌경사환자조중삽입형47례（88．68％），결실형위6례（11．32％），차이무통계학의의（P＞0．05）。건강대조조혈청ACE수평여뇌경사환자조비교차이유통계학의의（P＜0．05），뇌경사환자조저우건강대조조。결론 ACE기인rs4646994화rs35397082다태성여천진빈해지구건강인급급성뇌경사무현저관련，이자혈청수평차이불시유저량개위점차이소치，ACE기인적저량충SNP불시천진빈해지구급성뇌경사발생적독립위험인자。
Objective To study the association between the cerebral infarction and the angiotensin converting enzyme (ACE) gene rs4646994 and rs35397082 polymorphisms in Binhai area ,Tianjin .Methods Gene sequencing and DNA electrophoresis were used for the detection of the ACE gene single nucleotide polymorphisms (SNPs)(rs4646994 and rs35397082) .53 samples from pa‐tients with acute cerebral infarction and 53 samples from healthy volunteers were used in our study .Serum sample were collected from each group and tested by ACE ELISA .Results There were only deletion type of rs35397082 SNP in both of the control and cerebral infarction group .In the control group ,the number of insertion type of rs4646994 was 45(84 .91% ) ,deletion type was 8(15 . 09% ) and in the patients group ,the number of insertion was 47(88 .68% ) and the deletion was 6(11 .32% ) .There was no signifi‐cant difference between the patients group and the healthy donors (P>0 .05) .The concentration of ACE in control group was high‐er than the patients with acute cerebral infraction (P<0 .05) .Conclusion There is no significant association between the ACE gene polymorphisms(rs35397082 and rs4646994) and cerebral infarction in Binhai area ,Tianjin .The different concentration of ACE is not caused by these two SNPs .In this study ,these two SNPs are not the are not the risk factors of the cerebral infarction in Tianjin based on our study .