CAJ | 학술논문

目的：研究Th17细胞相关细胞因子IL‐17A、IL‐17F、IL‐22在隐球菌性脑膜炎患者发病机制中的作用。方法收集了2011年2月至2013年2月期间来院就诊的免疫功能正常的隐球菌患者22例以及同期来院体检的健康个体20例，收集外周抗凝血，分离了外周血单个核细胞，采用RT‐PCR以及ELISA检测了隐球菌性脑膜炎患者和健康对照者外周血单个核细胞（PB‐MC）中Th17相关细胞因子1L‐17A、IL‐17F以及IL‐22的mRNA表达以及血浆中各蛋白水平的表达。并分析其与脑脊液蛋白浓度、颅内压、脑脊液隐球菌抗原滴度、脑脊液葡萄糖浓度的关系。结果隐球菌性脑膜炎患者外周血单个核细胞中IL‐17A、IL‐22的mRNA水平以及血浆中IL‐17A、IL‐22水平较健康对照组显著增高（P＜0．01），且与隐球菌性脑膜炎患者的预后因子脑脊液蛋白浓度、颅内压、脑脊液隐球菌抗原滴度、脑脊液葡萄糖浓度相关。结论 T h17细胞相关细胞因子IL‐17A、IL‐22可能参与了隐球菌性脑膜炎的发病机制，可能是潜在的评价隐球菌性脑膜炎病情的指标。
목적：연구Th17세포상관세포인자IL‐17A、IL‐17F、IL‐22재은구균성뇌막염환자발병궤제중적작용。방법수집료2011년2월지2013년2월기간래원취진적면역공능정상적은구균환자22례이급동기래원체검적건강개체20례，수집외주항응혈，분리료외주혈단개핵세포，채용RT‐PCR이급ELISA검측료은구균성뇌막염환자화건강대조자외주혈단개핵세포（PB‐MC）중Th17상관세포인자1L‐17A、IL‐17F이급IL‐22적mRNA표체이급혈장중각단백수평적표체。병분석기여뇌척액단백농도、로내압、뇌척액은구균항원적도、뇌척액포도당농도적관계。결과은구균성뇌막염환자외주혈단개핵세포중IL‐17A、IL‐22적mRNA수평이급혈장중IL‐17A、IL‐22수평교건강대조조현저증고（P＜0．01），차여은구균성뇌막염환자적예후인자뇌척액단백농도、로내압、뇌척액은구균항원적도、뇌척액포도당농도상관。결론 T h17세포상관세포인자IL‐17A、IL‐22가능삼여료은구균성뇌막염적발병궤제，가능시잠재적평개은구균성뇌막염병정적지표。
Objective To investigate the IL‐17A/F and IL‐22 ,the Th17 related cytokine ,in the serum of cryptococcal meningitis patients and its clinical implications .Methods We collected peripheral blood from 22 cryptococcal meningitis patients and 20 matched healthy controls from February 2011 to February 2013 .Then the peripheral blood mononuclear cells(PBMC)were seperat‐ed with isodensity centrifugation .The RT‐PCR and ELISA were used to detect the mRNA and protein lever of Th17 related cyto‐kine such as IL‐17A ,IL‐17F and IL‐22 .In addition ,the relationship between these cytokines and cerebrospinal fluid characteristics were analyzed .Results The serum concentration of IL‐17A and IL‐22 were much higher than that of healthy controls (P<0 .01) . Their concentration were even higher in patients with poor prognosis factors ,including higher cerebrospinal fluid protein level and cryptococcal antigen titer ,lower cerebrospinal fluid glucose concentration and increased cerebrospinal fluid open pressure .Conclusion IL‐17A and IL‐22 were involved in the pathogenesis of cryptococcal meningitis and may be a potential prognosis biomarker for cryptococcal meningitis.