CAJ | 학술논문

目的：探讨新藤黄酸（GNA）对人肺癌细胞株A549的增值和凋亡的影响及其调控的可能机制。方法用不同浓度的GNA处理A549细胞，分为对照组和GNA组（GNA浓度分别为0．5、1．5、2．0 mg／L），采用MTT、细胞划痕、Hoechst染色法观察细胞的增殖、迁移和凋亡状况；Western blot检测凋亡相关蛋白caspase3、caspase9、p53和NF‐κB的表达变化。结果 GNA能明显抑制细胞的体外增殖和迁移且呈剂量依赖性；Hoechst染色显示GNA能诱导细胞凋亡，且随着浓度的升高凋亡现象越来越明显；Western blot显示GNA能上调促进凋亡的蛋白caspase3、caspase9、p53和下调NF‐κB的表达（P＜0．05）。结论 GNA能明显抑制人肺癌细胞株A549的增殖和迁移并诱导凋亡。
목적：탐토신등황산（GNA）대인폐암세포주A549적증치화조망적영향급기조공적가능궤제。방법용불동농도적GNA처리A549세포，분위대조조화GNA조（GNA농도분별위0．5、1．5、2．0 mg／L），채용MTT、세포화흔、Hoechst염색법관찰세포적증식、천이화조망상황；Western blot검측조망상관단백caspase3、caspase9、p53화NF‐κB적표체변화。결과 GNA능명현억제세포적체외증식화천이차정제량의뢰성；Hoechst염색현시GNA능유도세포조망，차수착농도적승고조망현상월래월명현；Western blot현시GNA능상조촉진조망적단백caspase3、caspase9、p53화하조NF‐κB적표체（P＜0．05）。결론 GNA능명현억제인폐암세포주A549적증식화천이병유도조망。
Objective To investigate the effects of gambogenic acid(GNA) on the proliferation and apoptosis of A549 lung canc‐er cells and its possible mechanism of regulation .Methods A549 cells were treated with different concentrations of GNA ,which di‐vided into control group and GNA group(0 .5 ,1 .5 ,20 mg/L) .The cell proliferation and apoptosis of A549 was analyzed by MTT , Hoechst staining ;The expression of apoptosis‐related protein caspase3 ,caspase9 ,p53 and NF‐κB were studied by Western blot .Re‐sults GNA could inhibit growth of A549 cells ;Moreover GNA could also induce apoptosis with the concentration increased more obviously ;Western blot showed that GNA down‐regulate NF‐κB ,increased caspase3 ,caspase9 and p53 expression(P<0 .05) .Con‐clusion GNA can efficiently promote the cell proliferation inhibition and the apoptosis of A 549 cells .