CAJ | 학술논문

目的：探讨儿童EB病毒（EBV ）感染者免疫功能状态及变化。方法采用免疫散射比浊法检测58例EB病毒衣壳抗原抗体IgM （EBV‐CA IgM ）阳性儿童血清免疫球蛋白，用流式细胞术检测外周血 T 淋巴细胞亚群 CD3＋、CD4＋、CD8＋、CD19＋，同期检测50例同龄健康儿童相同指标作为对照组。结果 EBNA‐IgG阴性组IgG、IgA较EBNA‐IgG阳性组和对照组明显降低，差异具有统计学意义（P＜0．05），而EBNA‐IgG阴性组、EBNA‐IgG阳性组血清总IgM和对照组比较，差异无统计学意义（P＞0．05）。EBNA‐IgG阴性组CD3＋、CD4＋、CD4＋／CD8＋、CD19＋较EBNA‐IgG阳性组和对照组明显降低，差异有统计学意义（P＜0．05），而EBNA‐IgG阴性组CD8＋较EBNA‐IgG阳性组和对照组明显升高，差异有统计学意义（P＜0．05）。结论 EBNA‐IgG阳性患儿免疫功能接近对照组优于EBNA‐IgG阴性患儿，EBNA‐IgG的出现可对患儿的免疫功能状况做初步的评估，通过检测免疫球蛋白和T细胞亚群、B淋巴细胞比例可了解儿童现症感染者免疫状态，对其感染阶段做进一步的评估。
목적：탐토인동EB병독（EBV ）감염자면역공능상태급변화。방법채용면역산사비탁법검측58례EB병독의각항원항체IgM （EBV‐CA IgM ）양성인동혈청면역구단백，용류식세포술검측외주혈 T 림파세포아군 CD3＋、CD4＋、CD8＋、CD19＋，동기검측50례동령건강인동상동지표작위대조조。결과 EBNA‐IgG음성조IgG、IgA교EBNA‐IgG양성조화대조조명현강저，차이구유통계학의의（P＜0．05），이EBNA‐IgG음성조、EBNA‐IgG양성조혈청총IgM화대조조비교，차이무통계학의의（P＞0．05）。EBNA‐IgG음성조CD3＋、CD4＋、CD4＋／CD8＋、CD19＋교EBNA‐IgG양성조화대조조명현강저，차이유통계학의의（P＜0．05），이EBNA‐IgG음성조CD8＋교EBNA‐IgG양성조화대조조명현승고，차이유통계학의의（P＜0．05）。결론 EBNA‐IgG양성환인면역공능접근대조조우우EBNA‐IgG음성환인，EBNA‐IgG적출현가대환인적면역공능상황주초보적평고，통과검측면역구단백화T세포아군、B림파세포비례가료해인동현증감염자면역상태，대기감염계단주진일보적평고。
Objective To explore children infected immune functional state and change .Methods using nephelometry in 58 ca‐ses of EBV‐IgM positive children serum immunoglobulin ,T lymphocyte subsets CD3+ ,CD4+ ,CD8+ ,CD19+ blood flow cytometry peripheral detection at the same period ,50 cases of healthy children as control group in the same index .Results EBNA‐IgG nega‐tive group of children IgG ,IgA was EBNA‐IgG positive group and control group decreased significantly ,the difference was statisti‐cally significant(P< 0 .05) ,The EBNA‐IgG negative group ,EBNA‐IgG positive group serum total IgM and control group com‐pared ,the difference was not statistically significant (P> 0 .05) .The EBNA‐IgG negative group of CD3+ ,CD4+ ,CD4+ /CD8+ , CD19+ in children with EBNA‐IgG positive group and control group decreased significantly ,the difference was statistically signifi‐cant(P<0 .05) ,while CD8+ was the EBNA‐IgG positive group and the control group increased significantly ,the difference was sta‐tistically significant(P<0 .05) .Conclusion EBNA‐IgG positive immune function of children with close to healthy group is better than that of EBNA‐IgG negative group ,the emergence of EBNA‐IgG status on immune function of children do preliminary assess‐ment ,through the detection of immunoglobulin and T cell subsets ,B lymphocyte ratio can understand the immune state of children′s infection ,further evaluation of infection phase .