CAJ | 학술논문

目的：评价Sysmex XN‐2000血细胞分析仪的体液模式对胸腔积液和腹腔积液中肿瘤细胞的筛查作用。方法用Sysmex XN‐2000血细胞分析仪体液模式测定84份腹腔积液和胸腔积液标本，分析其研究参数中高荧光细胞绝对值（HF‐BF＃）及比率（HF‐BF％）对腹腔积液和胸腔积液中肿瘤细胞的筛查作用，并对相关数据进行统计分析。结果在84例胸、腹腔积液标本中，脱落细胞学查到肿瘤细胞14例，其余70例未查到肿瘤细胞。14例恶性病例中，HF‐BF＃平均值为169／μL（0～2001／μL）， HF‐BF％平均值为29．7％（0％～261％）；70例良性病例中，HF‐BF＃平均值为39／μL （0～524／μL ），HF‐BF％平均值为4．2％（0％～27．8％），两组均值比较差异有统计学意义（P＜0．05）。用ROC 曲线分析高荧光细胞绝对值及比率对肿瘤细胞的检出能力可见HF‐BF＃曲线下面积（AUC）为0．493，而 HF‐BF％的 AUC为0．222。结论 Sysmex XN‐2000体液模式研究参数 HF‐BF＃及HF‐BF％虽可提供一定的肿瘤细胞筛选信息，但效果欠佳，在日常工作中不能过度依赖仪器的 HF‐BF＃、HF‐BF％等参数，对每一份样本均应进行涂片染色镜检，从而提高恶性细胞的检出率。
목적：평개Sysmex XN‐2000혈세포분석의적체액모식대흉강적액화복강적액중종류세포적사사작용。방법용Sysmex XN‐2000혈세포분석의체액모식측정84빈복강적액화흉강적액표본，분석기연구삼수중고형광세포절대치（HF‐BF＃）급비솔（HF‐BF％）대복강적액화흉강적액중종류세포적사사작용，병대상관수거진행통계분석。결과재84례흉、복강적액표본중，탈락세포학사도종류세포14례，기여70례미사도종류세포。14례악성병례중，HF‐BF＃평균치위169／μL（0～2001／μL）， HF‐BF％평균치위29．7％（0％～261％）；70례량성병례중，HF‐BF＃평균치위39／μL （0～524／μL ），HF‐BF％평균치위4．2％（0％～27．8％），량조균치비교차이유통계학의의（P＜0．05）。용ROC 곡선분석고형광세포절대치급비솔대종류세포적검출능력가견HF‐BF＃곡선하면적（AUC）위0．493，이 HF‐BF％적 AUC위0．222。결론 Sysmex XN‐2000체액모식연구삼수 HF‐BF＃급HF‐BF％수가제공일정적종류세포사선신식，단효과흠가，재일상공작중불능과도의뢰의기적 HF‐BF＃、HF‐BF％등삼수，대매일빈양본균응진행도편염색경검，종이제고악성세포적검출솔。
Objective To evaluate the capability for detecting of tumor cells in ascites and pleural effusion by Sysmex XN‐2000 hematology analyzer .Methods Determination of 84 samples of ascites and pleural effusion specimens with Sysmex XN‐2000 hema‐tology analyzer humoral model to analyze the study parameters highly fluorescent cells absolute value (HF‐BF # ) and percentage (HF‐BF% ) of ascites and pleural effusions screening of tumor cells ,and related data for statistical analysis .Results In 84 cases of pleural effusion and ascites samples ,cytology found 14 cases of malignant cells ,cancer cells are not found in 70 cases .14 cases of malignant cases ,HF‐BF # average of 169 /μL(0-2 001) ,HF‐BF% with an average of 29 .7% (0% -261% );70 cases of benign cases ,HF‐BF # an average of 39 /μL(0-524) ,HF‐BF% with an average of 4 .2% (0% -27 .8% ) ,both groups were statistical differences in the mean significance(P<0 .05) .ROC curve analysis with high absolute value and percentage of fluorescent cells to tumor cells detection function ,HF‐BF # area under the curve(AUC) was 0 .493 ,the area(AUC)% HF‐BF under the curve was 0 .222 .Conclusion Sysmex XN‐2000 Pattern of humoral parameters of HF‐BF # and HF‐BF% ,although the tumor cells may pro‐vide some screening information ,but ineffective ,so we believe that in our daily work can′t be over‐reliance on the instrument HF‐BF# ,HF‐BF% and other parameters should be performed on every sample smear staining ,thus improving the detection rate of ma‐lignant cells .