CAJ | 학술논문

目的：在蛋白质组水平上分析中国汉族缺陷型精神分裂症（DS ）与非缺陷型精神分裂症（NDS ）患者血清蛋白表达情况，从而寻找两种精神分裂症患者血清差异表达蛋白。方法利用双向电泳技术分析缺陷型精神分裂症与非缺陷型精神分裂症患者血清蛋白质组，并对差异表达的蛋白点进行质谱仪鉴定。结果两组血清蛋白二维蛋白图谱中存在18个显著差异表达的蛋白点，通过质谱鉴定出其中15个蛋白点。这15个蛋白中有12个蛋白在DS中下调，3个蛋白在DS中上调。结论首次利用蛋白质组学技术对中国汉族DS和NDS患者外周血蛋白表达谱进行比较研究，为开发DS特征性生物学标志及阐明缺陷型精神分裂症的病因学机制提供帮助。
목적：재단백질조수평상분석중국한족결함형정신분렬증（DS ）여비결함형정신분렬증（NDS ）환자혈청단백표체정황，종이심조량충정신분렬증환자혈청차이표체단백。방법이용쌍향전영기술분석결함형정신분렬증여비결함형정신분렬증환자혈청단백질조，병대차이표체적단백점진행질보의감정。결과량조혈청단백이유단백도보중존재18개현저차이표체적단백점，통과질보감정출기중15개단백점。저15개단백중유12개단백재DS중하조，3개단백재DS중상조。결론수차이용단백질조학기술대중국한족DS화NDS환자외주혈단백표체보진행비교연구，위개발DS특정성생물학표지급천명결함형정신분렬증적병인학궤제제공방조。
Objective To explore differently expressed proteins in serum samples obtained from Deficit Schizophrenia as well as Non‐Deficit Schizophrenia patients with proteomic techniques .Methods Make the comparative study of serum proteins between DS and NDS patients with two‐dimensional electrophoresis .The screened proteins were identified by mass spectrometer .Results Dur‐ing 18 differently expressed protein points screened in this study ,15 proteins were identified by mass spectrometer .Among of them , 12 proteins are up‐regulated while 3 proteins are down‐regulated in DS patients group .Conclusion It′s the first time to make the comparative study of serum protein profiles between DS and NDS patients by proteomic analysis .We expect that this study will contribute to better understanding of the pathophysiology of DS and explore the potential disease‐associated biomarker ,improve the future study for clinical medication of schizophrenia .