CAJ | 학술논문

目的：探讨胶质和钙结合EGF区域1（Collagen and calcium binding EGF domains 1,Ccbe1）病毒对人喉癌细胞系Hep-2细胞增殖及血管内皮生长因子-C(vessel endothelium growth factor C,VEGF-C)表达的影响。方法用1×102，1×104，1×106 pfu/mL的Ccbe1腺病毒作用喉癌Hep-2细胞，细胞分为对照组（PBS），Ccbe1病毒高、中、低剂量组。观察各组细胞增殖情况，采用PCR检测各组细胞VEGF-C mRNA的表达水平。结果与对照组比较，Ccbe1病毒各剂量组细胞增殖显著增高，VEGF-C mRNA表达水平显著上升，且均有显著剂量依赖性，差异有统计学意义(P<0.05)。结论Ccbe1病毒可能通过上调淋巴管生成因子VEGF-C表达促进喉癌细胞增殖和淋巴管生成。
목적：탐토효질화개결합EGF구역1（Collagen and calcium binding EGF domains 1,Ccbe1）병독대인후암세포계Hep-2세포증식급혈관내피생장인자-C(vessel endothelium growth factor C,VEGF-C)표체적영향。방법용1×102，1×104，1×106 pfu/mL적Ccbe1선병독작용후암Hep-2세포，세포분위대조조（PBS），Ccbe1병독고、중、저제량조。관찰각조세포증식정황，채용PCR검측각조세포VEGF-C mRNA적표체수평。결과여대조조비교，Ccbe1병독각제량조세포증식현저증고，VEGF-C mRNA표체수평현저상승，차균유현저제량의뢰성，차이유통계학의의(P<0.05)。결론Ccbe1병독가능통과상조림파관생성인자VEGF-C표체촉진후암세포증식화림파관생성。
Objective To investigate the impact on Vascular endothelial growth factor C (VEGF-C) expression of Collagen and calcium-binding EGF domain-containing protein 1 (Ccbe1) virus to laryngeal cancer Hep-2 Cells. Methods Cells were divided into control group (PBS), Ccbe1 virus in high, medium and low dose group, by the way of Ccbe1 adenovirus (in respective units 1í102, 1í104, 1í106pfu/mL) acting on laryngeal cancer Hep-2 Cells. The cell proliferation was observed and the expression level of VEGF-C mRNA was detected by PCR for each group. Results Compared with the control group, the cell proliferation of Ccbe1 virus was significantly higher in each group, and the VEGF-C mRNA expression level was significantly increased than that in control group (P<0.05), and there were significant dose-dependence. Conclusion Ccbe1 virus may upgrade Lymph vessel generation factor VEGF-C expression to promote hep-2 cells proliferation and lymphatic formation in laryngeal cancer.