齐齐哈尔医学院学报
齊齊哈爾醫學院學報
제제합이의학원학보
JOURNAL OF QIQIHAR MEDICAL COLLEGE
2015年
22期
3364-3365
,共2页
超高效液相色谱%质谱联用法%高良姜%高良姜素%含量测定
超高效液相色譜%質譜聯用法%高良薑%高良薑素%含量測定
초고효액상색보%질보련용법%고량강%고량강소%함량측정
UPLC-MS method%Alpinia officinarum Hance%Galangin%Content determination
目的：建立快速、简便的超高效液相色谱质谱联用法（ UPLC－MS）测定高良姜中高良姜素的含量。方法采用ACQUITY UPLC BEH C18（2．1 mm ×50 mm，1．7μm）色谱柱，以乙腈－0．1％甲酸水溶液流动相进行梯度洗脱；柱温30℃；流速0．3 ml／min；采用质谱一级全扫描方式、正离子模式检测。结果高良姜素在0．04～0．15 mg／ml之间与峰面积呈良好的线性关系，相应的回归方程为：y＝1．927×105x＋2．832×104（r＝0．9999）；平均回收率为97．85，RSD为2．43。结论该方法快速简便，准确可靠，灵敏度高，可应用于高良姜中高良姜素含量的快速检测及高良姜的质量控制。
目的：建立快速、簡便的超高效液相色譜質譜聯用法（ UPLC－MS）測定高良薑中高良薑素的含量。方法採用ACQUITY UPLC BEH C18（2．1 mm ×50 mm，1．7μm）色譜柱，以乙腈－0．1％甲痠水溶液流動相進行梯度洗脫；柱溫30℃；流速0．3 ml／min；採用質譜一級全掃描方式、正離子模式檢測。結果高良薑素在0．04～0．15 mg／ml之間與峰麵積呈良好的線性關繫，相應的迴歸方程為：y＝1．927×105x＋2．832×104（r＝0．9999）；平均迴收率為97．85，RSD為2．43。結論該方法快速簡便，準確可靠，靈敏度高，可應用于高良薑中高良薑素含量的快速檢測及高良薑的質量控製。
목적：건립쾌속、간편적초고효액상색보질보련용법（ UPLC－MS）측정고량강중고량강소적함량。방법채용ACQUITY UPLC BEH C18（2．1 mm ×50 mm，1．7μm）색보주，이을정－0．1％갑산수용액류동상진행제도세탈；주온30℃；류속0．3 ml／min；채용질보일급전소묘방식、정리자모식검측。결과고량강소재0．04～0．15 mg／ml지간여봉면적정량호적선성관계，상응적회귀방정위：y＝1．927×105x＋2．832×104（r＝0．9999）；평균회수솔위97．85，RSD위2．43。결론해방법쾌속간편，준학가고，령민도고，가응용우고량강중고량강소함량적쾌속검측급고량강적질량공제。
Objective To establish a rapid and sensitive ultra performance liquid chromatography-mass spectrometry ( UPLC-MS) method for the determination of galangin in Alpinia officinarum Hance .Methods ACQUITY UPLC BEH C18 (2.1 mm ×50 mm, 1.7μm) column was used with acetonitrile -0.1%as the formic acid mobile phase in gradient elution .The column temperature was 30℃, and the flow rate was 0.3 ml? min-1 . Full scan MS and positive ion mode detection were used .Results Galangin showed a good linear relationship in the range of 0.04~0.15 mg? ml-1 .The linear regression equation of galangin was y =1.927 ×105 x+2.832 × 104(r=0.999 9).The average recoveries was 97.85; RSD was 2.43.Conclusions The method is simple, rapid, sensitive, accurate and reliable , which can be applied in the rapid determination of active ingredients and quality control in Alpinia officinarum Hance .
