酿酒科技
釀酒科技
양주과기
LIQUOR-MAKING SCIENCE & TECHNOLOGY
2015年
8期
39-42
,共4页
常压室温等离子体诱变系统(ARTP)%枯草芽孢杆菌%四甲基吡嗪(TTMP)%选育
常壓室溫等離子體誘變繫統(ARTP)%枯草芽孢桿菌%四甲基吡嗪(TTMP)%選育
상압실온등리자체유변계통(ARTP)%고초아포간균%사갑기필진(TTMP)%선육
ARTP%Bacillus subtilis%TTMP%breeding
从新疆伊犁肖尔布拉克酒厂生产的酱香型高温大曲中筛选得1株能代谢四甲基吡嗪（TTMP）的枯草芽孢杆菌(Bacillus subtilis)R19，以该菌株为出发菌株，采用常压室温等离子体诱变系统（ARTP）进行诱变，根据脱脂奶粉平板初筛、摇瓶发酵复筛，连续传代培养后得到遗传稳定的TTMP产量较高的突变株T451，该菌株摇瓶发酵生产TTMP的产量为1.19 g/L，约为出发菌株产量的4.45倍。
從新疆伊犛肖爾佈拉剋酒廠生產的醬香型高溫大麯中篩選得1株能代謝四甲基吡嗪（TTMP）的枯草芽孢桿菌(Bacillus subtilis)R19，以該菌株為齣髮菌株，採用常壓室溫等離子體誘變繫統（ARTP）進行誘變，根據脫脂奶粉平闆初篩、搖瓶髮酵複篩，連續傳代培養後得到遺傳穩定的TTMP產量較高的突變株T451，該菌株搖瓶髮酵生產TTMP的產量為1.19 g/L，約為齣髮菌株產量的4.45倍。
종신강이리초이포랍극주엄생산적장향형고온대곡중사선득1주능대사사갑기필진（TTMP）적고초아포간균(Bacillus subtilis)R19，이해균주위출발균주，채용상압실온등리자체유변계통（ARTP）진행유변，근거탈지내분평판초사、요병발효복사，련속전대배양후득도유전은정적TTMP산량교고적돌변주T451，해균주요병발효생산TTMP적산량위1.19 g/L，약위출발균주산량적4.45배。
In this study, a TTMP-producing Bacillus subtilis strain R19 was screened from Jiangxiang high-temperature Daqu in Xiaoerbulake Distillery in Xinjiang. Then R19 was used as the starting strain to obtain its mutant strains through atmospheric room temperature plasma (ARTP). And a mutant strain T451 with high yield of TTMP and stable heredity was obtained through plat screening and flask fermentation. TT-MP yield of T451 in flask fermentation was as high as 1.19 g/L, 4.45 times of that of the starting strain.
