CAJ | 학술논문

双乙酰是啤酒发酵过程中酵母自身代谢产生的一种副产物，其含量是衡量啤酒成熟的主要指标。双乙酰含量的传统检测方法（VDK检测方法）存在一定的缺陷，而总双乙酰（TVDK）是双乙酰及其前驱体的总和，用TVDK来评价啤酒的成熟度具有正确的指导价值。本试验通过验证VDK检测方法中存在的缺陷，得出TVDK方法的可行性，并且研究了不同浓度啤酒发酵过程中TVDK的变化规律，通过阈值试验得出TVDK在啤酒发酵成熟评价中的控制标准为0.10 mg/L。试验提出了测定啤酒中总双乙酰的可行性方法，对保证啤酒的正常发酵起到了一定作用。
쌍을선시비주발효과정중효모자신대사산생적일충부산물，기함량시형량비주성숙적주요지표。쌍을선함량적전통검측방법（VDK검측방법）존재일정적결함，이총쌍을선（TVDK）시쌍을선급기전구체적총화，용TVDK래평개비주적성숙도구유정학적지도개치。본시험통과험증VDK검측방법중존재적결함，득출TVDK방법적가행성，병차연구료불동농도비주발효과정중TVDK적변화규률，통과역치시험득출TVDK재비주발효성숙평개중적공제표준위0.10 mg/L。시험제출료측정비주중총쌍을선적가행성방법，대보증비주적정상발효기도료일정작용。
Diacetyl, a by-product of yeast metabolism in beer fermentation process, its content is a main indicator for evaluating beer fermenta-tion maturity. Traditional VDK measurement for diacetyl content has certain defects. TVDK is the sum of diacetyl and its precursor, and the measurement of TVDK to evaluate beer fermentation maturity is scientific. In this study, the feasibility of TVDK measurement was demon-strated through the validation of the existed defects of VDK measurement, and the change rules of TVDK in the fermentation process of beer of different alcohol content were investigated. The control standards of TVDK in the evaluation on beer fermentation maturity were determined as 100 ppb by threshold test. The feasible methods for the measurement of TVDK in beer were put forward which could guarantee normal beer fer-mentation.