CAJ | 학술논문

目的：探讨经过GW3965预处理激动肝X受体（LXR）后对SD大鼠肝移植缺血再灌注损伤（IRI）的保护作用及其机制。方法70只雄性、健康的SD大鼠，分为假手术组（SO组，14只）GW3965预处理组（GW3965组，14对）和原位肝移植组（OLT组，14对）；术后分别检测各时相点的血清转氨酶、肝组织病理改变、血浆炎症因子（TNF‐α、IL‐1）水平，肝病理组织中NF‐κB表达水平。结果再灌注6、24 h后原位肝移植组与GW3965组血浆炎症因子表达水平、血清转氨酶、肝脏组织病理损伤程度、核因子κB （NF‐κB）表达水平均高于 SO组；而再灌注6、24 h后，GW3965组血清转氨酶、血浆炎症因子表达水平、肝脏组织病理损伤、NF‐κB表达水平低于OLT组，差异有统计学意义（P＜0．05）。结论预先经过GW3965激动供肝的LXR后可以有效降低肝移植再灌注期肝脏内毒素信号通路中的核因子κB NF‐κB表达和其下游炎症肿瘤坏死因子α（TNF‐α）、白细胞介素1（IL‐1）因子的产生，从而有效保护移植供肝缺血再灌注的损伤。
목적：탐토경과GW3965예처리격동간X수체（LXR）후대SD대서간이식결혈재관주손상（IRI）적보호작용급기궤제。방법70지웅성、건강적SD대서，분위가수술조（SO조，14지）GW3965예처리조（GW3965조，14대）화원위간이식조（OLT조，14대）；술후분별검측각시상점적혈청전안매、간조직병리개변、혈장염증인자（TNF‐α、IL‐1）수평，간병리조직중NF‐κB표체수평。결과재관주6、24 h후원위간이식조여GW3965조혈장염증인자표체수평、혈청전안매、간장조직병리손상정도、핵인자κB （NF‐κB）표체수평균고우 SO조；이재관주6、24 h후，GW3965조혈청전안매、혈장염증인자표체수평、간장조직병리손상、NF‐κB표체수평저우OLT조，차이유통계학의의（P＜0．05）。결론예선경과GW3965격동공간적LXR후가이유효강저간이식재관주기간장내독소신호통로중적핵인자κB NF‐κB표체화기하유염증종류배사인자α（TNF‐α）、백세포개소1（IL‐1）인자적산생，종이유효보호이식공간결혈재관주적손상。
Objective To study the protective effect and mechanism of SD rats′liver transplantation reperfusion ischemia‐reper‐fusion injury(IRI) after GW3965 activation of liver X receptor preprocessing .Methods Separated Male SD(Sprague‐Dawley) 70 rats into 3 groups which were sham operation group (SO group ,14 rats) ,orthotopic liver transplantation group (OLT group ,28 rats) ,and GW 3965 preprocessing group(GW 3965 group 28rats) .The levels of serum transaminase ,plasma inflammatory factors (TNF‐α、IL‐1) ,the changes of hepatic pathology and inflammatory factor mRNA ,and the activities as well as its expressions of NF‐κB in hepatic tissue were observed ,after the operation .Results After 6 and 24 hours perfusion ,the levels of plasma inflammatory factors was expression ,serum transaminase ,the liver pathological injury degree and the activities as well as its expressions of NF‐κB in OLT group and GW3965 group were higher than those in SO group .While after reperfusion for 6 and 24 hours ,the levels of ser‐um transaminase ,plasma inflammatory factors expression ,the liver pathological injury degree ,inflammatory factor and the activities as well as its expressions of NF‐κB in GW3965 group were much lower than those in OLT group ,there were obvious differences (P<0 .05) .Conclusion After GW3965 activation of liver X receptor preprocessing ,the activities of NF‐κB and the emerging of downstream inflammatory mediator factors are reduced effectively and protect the liver after the ischemia reperfusion .