CAJ | 학술논문

目的：研究和评价实用单克隆抗体SPA红细胞花环直接法（简称实用McAb‐A‐E直接法）检验 T淋巴细胞亚群细胞涂片细胞计数随机误差，并与M cA b‐A‐E直接法原方法比较。方法随机选取20例样本，在涂片的2／6～4／6区域间至少2个区计数200个细胞，计数区域不重复，CD4＋和CD8＋连续计数4次，CD3＋连续计数2次。结果实用M cA b‐A‐E直接法细胞计数随机误差分别为CD3＋：7．86，CD4＋：9．99，CD8＋：8．55，CD4＋／CD8＋：0．33，低于 M cA b‐A‐E直接法原方法误差，即CD3＋：15．00，CD4＋：13．80，CD8＋：10．70，CD4＋／CD8＋：0．69。结论实用M cA b‐A‐E直接法细胞计数随机误差小于M cA b‐A‐E直接法原方法。
목적：연구화평개실용단극륭항체SPA홍세포화배직접법（간칭실용McAb‐A‐E직접법）검험 T림파세포아군세포도편세포계수수궤오차，병여M cA b‐A‐E직접법원방법비교。방법수궤선취20례양본，재도편적2／6～4／6구역간지소2개구계수200개세포，계수구역불중복，CD4＋화CD8＋련속계수4차，CD3＋련속계수2차。결과실용M cA b‐A‐E직접법세포계수수궤오차분별위CD3＋：7．86，CD4＋：9．99，CD8＋：8．55，CD4＋／CD8＋：0．33，저우 M cA b‐A‐E직접법원방법오차，즉CD3＋：15．00，CD4＋：13．80，CD8＋：10．70，CD4＋／CD8＋：0．69。결론실용M cA b‐A‐E직접법세포계수수궤오차소우M cA b‐A‐E직접법원방법。
Objective To research and evaluate cell counting random error for cell sheet of T lymphocyte subsets in the practical McAb‐A‐E direct method ,and old method of McAb‐A‐E direct method compared .Methods Randomly selected 20 specimens ,cell sheet of T lymphocyte subsets on counting 200 cell at least 2 area from 2/6~4/6 area ,counting area non‐repeated ,CD4+ and CD8+continuous counter 4 times ,CD3+ continuous counter 2 times .Results In the practical McAb‐A‐E direct method ,random error mean of cell counting was separately CD3+ :7 .86 ,CD4+ :9 .99 ,CD8+ :8 .55 ,CD4+ /CD8+ :0 .33 ,lower than old method of McAb‐A‐E direct method ,that was CD3+ :15 .00 ,CD4+ :13 .80 ,CD8+ :10 .70 ,CD4+ /CD8+ :0 .69 .Conclusion Cell counting random error in practical McAb‐A‐E direct method for cell sheet of T lymphocyte subsets is less than old method of McAb‐A‐E direct method .