蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2015年
7期
863-865
,共3页
朱卫香%刘彦敏%张砚卿%杜国辉%张琳琳
硃衛香%劉彥敏%張硯卿%杜國輝%張琳琳
주위향%류언민%장연경%두국휘%장림림
脑血管意外%对氧磷酯酶-1%氧化低密度脂蛋白
腦血管意外%對氧燐酯酶-1%氧化低密度脂蛋白
뇌혈관의외%대양린지매-1%양화저밀도지단백
cerebralvascular accident%serum paraoxonase-1%oxidized low-density lipoprotein
目的：：探讨急性缺血性脑卒中患者血清对氧磷酯酶-1(PON-1)活性和氧化低密度脂蛋白(ox-LDL)水平的变化及其临床意义。方法：选择急性缺血性脑卒中患者96例及正常对照组72名,采用对硝基苯酚为底物的速率法和酶联免疫吸附法分别测定血清PON-1、ox-LDL,同时检测2组的总胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇等生化指标并进行对比；探讨PON-1、ox-LDL与急性缺血性脑卒中的关系。结果：缺血性脑卒中组血清PON-1和高密度脂蛋白胆固醇均明显低于对照组,ox-LDL和低密度脂蛋白胆固醇明显高于对照组(P<0.01)；大梗死组血清PON-1活性均低于中梗死组和小梗死组(P<0.05和P<0.01)；而大梗死组ox-LDL水平均明显高于中梗死组和小梗死组(P<0.01)；神经功能重度损伤组患者血清PON-1活性均明显低于轻、中度损伤组(P<0.01),而血清ox-LDL水平均显著高于轻、中度损伤组(P<0.01)。相关分析显示：PON-1与ox-LDL呈负相关关系(P<0.01)。结论：急性缺血性脑卒中患者PON-1活性降低,导致抗氧化能力减弱,使血清ox-LDL水平增加,这种改变可能是缺血性脑卒中重要的危险因素,对预测脑卒中风险有重要意义。
目的：：探討急性缺血性腦卒中患者血清對氧燐酯酶-1(PON-1)活性和氧化低密度脂蛋白(ox-LDL)水平的變化及其臨床意義。方法：選擇急性缺血性腦卒中患者96例及正常對照組72名,採用對硝基苯酚為底物的速率法和酶聯免疫吸附法分彆測定血清PON-1、ox-LDL,同時檢測2組的總膽固醇、三酰甘油、低密度脂蛋白膽固醇、高密度脂蛋白膽固醇等生化指標併進行對比；探討PON-1、ox-LDL與急性缺血性腦卒中的關繫。結果：缺血性腦卒中組血清PON-1和高密度脂蛋白膽固醇均明顯低于對照組,ox-LDL和低密度脂蛋白膽固醇明顯高于對照組(P<0.01)；大梗死組血清PON-1活性均低于中梗死組和小梗死組(P<0.05和P<0.01)；而大梗死組ox-LDL水平均明顯高于中梗死組和小梗死組(P<0.01)；神經功能重度損傷組患者血清PON-1活性均明顯低于輕、中度損傷組(P<0.01),而血清ox-LDL水平均顯著高于輕、中度損傷組(P<0.01)。相關分析顯示：PON-1與ox-LDL呈負相關關繫(P<0.01)。結論：急性缺血性腦卒中患者PON-1活性降低,導緻抗氧化能力減弱,使血清ox-LDL水平增加,這種改變可能是缺血性腦卒中重要的危險因素,對預測腦卒中風險有重要意義。
목적：：탐토급성결혈성뇌졸중환자혈청대양린지매-1(PON-1)활성화양화저밀도지단백(ox-LDL)수평적변화급기림상의의。방법：선택급성결혈성뇌졸중환자96례급정상대조조72명,채용대초기분분위저물적속솔법화매련면역흡부법분별측정혈청PON-1、ox-LDL,동시검측2조적총담고순、삼선감유、저밀도지단백담고순、고밀도지단백담고순등생화지표병진행대비；탐토PON-1、ox-LDL여급성결혈성뇌졸중적관계。결과：결혈성뇌졸중조혈청PON-1화고밀도지단백담고순균명현저우대조조,ox-LDL화저밀도지단백담고순명현고우대조조(P<0.01)；대경사조혈청PON-1활성균저우중경사조화소경사조(P<0.05화P<0.01)；이대경사조ox-LDL수평균명현고우중경사조화소경사조(P<0.01)；신경공능중도손상조환자혈청PON-1활성균명현저우경、중도손상조(P<0.01),이혈청ox-LDL수평균현저고우경、중도손상조(P<0.01)。상관분석현시：PON-1여ox-LDL정부상관관계(P<0.01)。결론：급성결혈성뇌졸중환자PON-1활성강저,도치항양화능력감약,사혈청ox-LDL수평증가,저충개변가능시결혈성뇌졸중중요적위험인소,대예측뇌졸중풍험유중요의의。
Objective:To investigate the changes of serum paraoxonase-1(PON-1) activity,oxidized low-density lipoprotein(ox-LDL) level in acute ischemic stroke patients and analyze the clinical significance. Methods:Ninety-six acute ischemic stroke cases and 72 health subjects were selected. Serum PON-1 activity was measured using paroxon as substrate and ox-LDL level was detected by enzyme-linked immuno sorbent assay method. Total cholesterol, triglyceride, high-density lipoprotein-cholesterol and low-density lipoprotein-cholesterol levels were all measured in the two groups. The relationships of serum PON-1 activity,ox-LDL level and acute ischemic stroke were explored. Results:In ischemic stroke group,serum PON-1 activity and high-density lipoprotein-cholesterol level were significantly lower than those in control group( P <0. 01). Serum ox-LDL and low-density lipoprotein-cholesterol levels were significantly higher in ischemic stroke group than in control group( P<0. 01). Serum PON-1 activity was significantly lower in patients with large cerebral infarction than that in patients with medium and small cerebral infarction(P<0. 05 and P<0. 01). Serum ox-LDL level was significantly higher in patients with large cerebral infarction than that in patients with medium and small cerebral infarction (P<0. 01). Serum PON-1 activity was significantly lower in patients with severe neurologic impairment than that in patients with mild and moderate neurologic impairment (P<0. 01). Serum ox-LDL level was significantly higher in the patients with severe neurologic impairment than that in the patients with mild and moderate neurologic impairment(P<0. 01). Based on the correlation analysis,PON-1 activity was correlated negatively with ox-LDL(P<0. 01). Conclusions:In the patients with acute ischemic stroke,serum PON-1 activity was decreased and ox-LDL level was increased, the changes may be an important risk factor for ischemic stroke. It has an important meaning to forecast the risk of ischemic stroke.
