国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
13期
968-972
,共5页
慢性阻塞性肺疾病%香烟烟雾%T辅助细胞17
慢性阻塞性肺疾病%香煙煙霧%T輔助細胞17
만성조새성폐질병%향연연무%T보조세포17
Chronic obstructive pulmonary disease%Tobacco smoke%Th17 cells
目的 检测吸烟致COPD患者肺组织T辅助细胞17(Th17)分布及IL-17水平变化,探讨吸烟导致的COPD慢性炎症反应的可能机制.方法 收集2013年9月至2014年3月在我院因“孤立性肺结节”行手术切除肺叶患者的肺组织标本共56例,并将其分组:吸烟COPD组、吸烟肺功能正常组、不吸烟COPD组、不吸烟肺功能正常组.收集各组患者肺组织标本,HE染色法观察肺组织形态,实时荧光定量PCR方法检测Th17转录因子维甲酸受体相关孤儿受体t(RORγt)mRNA及IL-17 mRNA在肺组织的转录水平,ELISA法检测IL 17因子水平.结果 吸烟COPD组气道炎症病理评分明显增高,其RORγt、IL-17mRNA水平及IL-17均高于另外3组(P＜0.05);吸烟肺功能正常组较非吸烟肺功能正常组气道炎症水及RORγt、IL 17mRNA及IL 17细胞因子水平增高.结论 吸烟相关COPD患者肺组织中存在Th17优势表达,IL-17表达水平与气道慢性炎症相关,Th17可能在吸烟所致COPD的发病机制中发挥重要作用.
目的 檢測吸煙緻COPD患者肺組織T輔助細胞17(Th17)分佈及IL-17水平變化,探討吸煙導緻的COPD慢性炎癥反應的可能機製.方法 收集2013年9月至2014年3月在我院因“孤立性肺結節”行手術切除肺葉患者的肺組織標本共56例,併將其分組:吸煙COPD組、吸煙肺功能正常組、不吸煙COPD組、不吸煙肺功能正常組.收集各組患者肺組織標本,HE染色法觀察肺組織形態,實時熒光定量PCR方法檢測Th17轉錄因子維甲痠受體相關孤兒受體t(RORγt)mRNA及IL-17 mRNA在肺組織的轉錄水平,ELISA法檢測IL 17因子水平.結果 吸煙COPD組氣道炎癥病理評分明顯增高,其RORγt、IL-17mRNA水平及IL-17均高于另外3組(P＜0.05);吸煙肺功能正常組較非吸煙肺功能正常組氣道炎癥水及RORγt、IL 17mRNA及IL 17細胞因子水平增高.結論 吸煙相關COPD患者肺組織中存在Th17優勢錶達,IL-17錶達水平與氣道慢性炎癥相關,Th17可能在吸煙所緻COPD的髮病機製中髮揮重要作用.
목적 검측흡연치COPD환자폐조직T보조세포17(Th17)분포급IL-17수평변화,탐토흡연도치적COPD만성염증반응적가능궤제.방법 수집2013년9월지2014년3월재아원인“고립성폐결절”행수술절제폐협환자적폐조직표본공56례,병장기분조:흡연COPD조、흡연폐공능정상조、불흡연COPD조、불흡연폐공능정상조.수집각조환자폐조직표본,HE염색법관찰폐조직형태,실시형광정량PCR방법검측Th17전록인자유갑산수체상관고인수체t(RORγt)mRNA급IL-17 mRNA재폐조직적전록수평,ELISA법검측IL 17인자수평.결과 흡연COPD조기도염증병리평분명현증고,기RORγt、IL-17mRNA수평급IL-17균고우령외3조(P＜0.05);흡연폐공능정상조교비흡연폐공능정상조기도염증수급RORγt、IL 17mRNA급IL 17세포인자수평증고.결론 흡연상관COPD환자폐조직중존재Th17우세표체,IL-17표체수평여기도만성염증상관,Th17가능재흡연소치COPD적발병궤제중발휘중요작용.
Objective To investigate the Th17 cell distribution and expression of IL-17 in tobaccosmoke correlated COPD patients lung tissue.Methods Fifty-six cases of underwent solitary pulmonary nodules lobectomy from Peking University Shenzhen Hospital from September 2013 to March 2014 were recruited in this study.They were devided into smoked COPD group,smoked with normal lung function group,non smoked COPD group,non smoked with normal lung function group.All of lung tissue samples were collected,observed the lung tissue morphology by HE staining.The expression of Th17 specific transcription factor RORγt and IL-17 at mRNA level in lung tissue were determined by quantitative rea1-time PCR.The concentrations of IL-17 cytokines in lung samples werc measured by enzyme-linked immunosorbent assay (ELISA).Results Compared with the other three groups,smoked COPD group had dramatically increased airway inflammatory pathologic score,and showed higher level of the expression of RORγt,IL-17mRNA and IL 17 cytokine concentration.In smoked with normal lung function group,RORγt,IL-17 were up-regulated at transcriptional leve1,IL-17 cytokine concentration also increased than the other two non smoked group.Conclusions Th17 cell preferentially expressed in COPD patients lung tissue,the effective cytokine level of IL-17 was correlated with severity of chronic inflammation.Th17 maybe play an important role in the pathogenesis in chronic obstructive pulmonary disease.
