国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
13期
994-999
,共6页
张晓雷%侯晓莉%庞宝森%王军
張曉雷%侯曉莉%龐寶森%王軍
장효뢰%후효리%방보삼%왕군
肺气肿%外周骨骼肌%肌肉功能异常%卡托普利
肺氣腫%外週骨骼肌%肌肉功能異常%卡託普利
폐기종%외주골격기%기육공능이상%잡탁보리
Emphysema%Peripheral skeletal muscle%Muscle dysfunction%Captopril
目的 探讨卡托普利对肺气肿大鼠外周骨骼肌生物力学、病理形态学和氧化代谢的影响.方法 将40只雄性SD大鼠随机分为正常对照组、卡托普利对照组、肺气肿组、肺气肿卡托普利干预组,每组10只.采用气管内滴注猪胰弹性蛋白酶方法复制大鼠肺气肿模型.卡托普利对照组和卡托普利干预组大鼠给予含50 mg/L卡托普利的自来水.于模型复制20周末时,行大鼠原位腓肠肌生物力学测定和心脏解剖学指标评价;并检测肌纤维组分构成,毛细血管密度,肌细胞内脂褐素包涵体的含量以及肌肉组织匀浆中氧化代谢酶活性变化.结果 卡托普利干预组大鼠腓肠肌抗疲劳耐力显著高于肺气肿组,肌力半数恢复时间[(96.7±44.2)s]较肺气肿组[(145.0±55.4)s]缩短(P＜0.05);卡托普利干预组大鼠腓肠肌Ⅰ型纤维的比例[(24.8±4.4)％]较肺气肿组[(16.0±5.0)％]增加(P＜0.05),Ⅱ型肌纤维的比例[(75.2±7.2)％]较肺气肿组[(84.0±9.4)％]降低(P＜0.05),2组大鼠腓肠肌毛细血管密度差异无统计学意义(P＞0.05);卡托普利干预组大鼠腓肠肌脂褐素包涵体的含量(2.4±0.6)较肺气肿组(3.2±0.4)降低(P＜0.05),抗氧化酶活性高于肺气肿组(P＜0.05);卡托普利干预组大鼠肺组织病理形态学、血压、左右心室质量与肺气肿组差异无统计学意义(P＞0.05).结论 卡托普利可改善肺气肿大鼠外周骨骼肌纤维组分构成、提高抗氧化能力、减轻氧化损伤,改善疲劳耐力.
目的 探討卡託普利對肺氣腫大鼠外週骨骼肌生物力學、病理形態學和氧化代謝的影響.方法 將40隻雄性SD大鼠隨機分為正常對照組、卡託普利對照組、肺氣腫組、肺氣腫卡託普利榦預組,每組10隻.採用氣管內滴註豬胰彈性蛋白酶方法複製大鼠肺氣腫模型.卡託普利對照組和卡託普利榦預組大鼠給予含50 mg/L卡託普利的自來水.于模型複製20週末時,行大鼠原位腓腸肌生物力學測定和心髒解剖學指標評價;併檢測肌纖維組分構成,毛細血管密度,肌細胞內脂褐素包涵體的含量以及肌肉組織勻漿中氧化代謝酶活性變化.結果 卡託普利榦預組大鼠腓腸肌抗疲勞耐力顯著高于肺氣腫組,肌力半數恢複時間[(96.7±44.2)s]較肺氣腫組[(145.0±55.4)s]縮短(P＜0.05);卡託普利榦預組大鼠腓腸肌Ⅰ型纖維的比例[(24.8±4.4)％]較肺氣腫組[(16.0±5.0)％]增加(P＜0.05),Ⅱ型肌纖維的比例[(75.2±7.2)％]較肺氣腫組[(84.0±9.4)％]降低(P＜0.05),2組大鼠腓腸肌毛細血管密度差異無統計學意義(P＞0.05);卡託普利榦預組大鼠腓腸肌脂褐素包涵體的含量(2.4±0.6)較肺氣腫組(3.2±0.4)降低(P＜0.05),抗氧化酶活性高于肺氣腫組(P＜0.05);卡託普利榦預組大鼠肺組織病理形態學、血壓、左右心室質量與肺氣腫組差異無統計學意義(P＞0.05).結論 卡託普利可改善肺氣腫大鼠外週骨骼肌纖維組分構成、提高抗氧化能力、減輕氧化損傷,改善疲勞耐力.
목적 탐토잡탁보리대폐기종대서외주골격기생물역학、병리형태학화양화대사적영향.방법 장40지웅성SD대서수궤분위정상대조조、잡탁보리대조조、폐기종조、폐기종잡탁보리간예조,매조10지.채용기관내적주저이탄성단백매방법복제대서폐기종모형.잡탁보리대조조화잡탁보리간예조대서급여함50 mg/L잡탁보리적자래수.우모형복제20주말시,행대서원위비장기생물역학측정화심장해부학지표평개;병검측기섬유조분구성,모세혈관밀도,기세포내지갈소포함체적함량이급기육조직균장중양화대사매활성변화.결과 잡탁보리간예조대서비장기항피로내력현저고우폐기종조,기력반수회복시간[(96.7±44.2)s]교폐기종조[(145.0±55.4)s]축단(P＜0.05);잡탁보리간예조대서비장기Ⅰ형섬유적비례[(24.8±4.4)％]교폐기종조[(16.0±5.0)％]증가(P＜0.05),Ⅱ형기섬유적비례[(75.2±7.2)％]교폐기종조[(84.0±9.4)％]강저(P＜0.05),2조대서비장기모세혈관밀도차이무통계학의의(P＞0.05);잡탁보리간예조대서비장기지갈소포함체적함량(2.4±0.6)교폐기종조(3.2±0.4)강저(P＜0.05),항양화매활성고우폐기종조(P＜0.05);잡탁보리간예조대서폐조직병리형태학、혈압、좌우심실질량여폐기종조차이무통계학의의(P＞0.05).결론 잡탁보리가개선폐기종대서외주골격기섬유조분구성、제고항양화능력、감경양화손상,개선피로내력.
Objective To study the effects of captopril on peripheral skeletal muscle biomechanics,pathomorphology and oxidative metabolism in rats with emphysema.Methods The emphysema rat model was established by intracheal instillation of porcine pancreatic elastase.Forty male Sprague-Dawley rats were randomly divided into 4 groups (n=10 each):a normal control group,a eaptopril control group (captopril 50 mg/L in drinking water),a emphysema group,and a captopril intervention group (emphysema model rats receiving captopril 50 mg/L in drinking water).Twenty weeks after instillation,in situ mechanical properties of gastrocnemius and cardiac anatomic indexes were evaluated.Gastrocnemius fiber type composition and capillary density (CD) were assessed by using ATPase staining.Lipofuscin inclusions (LI/F) were determined with the ferric ferricyanide reduction test technique.The muscle biopsy homogenate was used to measure the activity of superoxide dismutase (SOD),catalase (CAT) and total antioxidant capacity (T-AOC).Results Captopril increased antifatigue tolerance and the force recovery rate of gastrocnemius muscle [(96.7±44.2)s vs(145.0±55.4)s,P ＜ 0.05] in rats with emphysema.Captopril increased the proportion of type Ⅰ fibers [(24.8±4.4)％ vs (16.0±5.0),P ＜0.05],with a reciprocal decrease in type Ⅱ fibers [(75.2±7.2) ％ vs (84.0±9.4) ％,P ＜0.05] in rats with emphysema,but had no effects on gastrocnemius CD.Captopril decreased LI/F in thegastrocnemius muscle of emphysema rats (2.4±0.6 vs 3.2±0.4,P ＜0.05),and elevated the activity of SOD[(72.4±18.3)U/mg vs (55.3±16.2) U/mg,P ＜0.05],CAT [(14.3±5.4)U/mg provs (9.3±3.7) U/mg pro,P ＜0.05]and T-AOC [(79.2±16.7) nmol/mg pro vs (62.1±13.4) nmol/mg pro,P ＜0.05] in muscle homogenate of rats with emphysem.Captopril had no effect on pulmonary pathomorphologic or cardiac anatomic indices in rats with emphysema.Conclusions Captopril could enhance the antifatigue tolerance,improve the fiber type constitution,lower lipofusin inclusions and increase the antioxidant enzyme activities in the peripheral skeletal muscle of rats with emphysema.
