中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
30期
2447-2450
,共4页
岳常丽%朴颖实%白玉萍%刘红刚%张罗
嶽常麗%樸穎實%白玉萍%劉紅剛%張囉
악상려%박영실%백옥평%류홍강%장라
鼻肿瘤%鼻窦肿瘤%肠型腺癌%涎腺型肿瘤%非肠型腺癌
鼻腫瘤%鼻竇腫瘤%腸型腺癌%涎腺型腫瘤%非腸型腺癌
비종류%비두종류%장형선암%연선형종류%비장형선암
Nose neoplasms%Paranasal sinus neoplasms%Intestinal-type adenocarcinoma%Salivary gland-type neoplasms%Non-intestinal sinonasal adenocarcinoma
目的 研究细胞角蛋白(CK)7、CK20、转录因子SOX10和尾型同源核转录因子2(CDX2)在鼻腔鼻窦原发腺癌中的表达及其诊断和鉴别诊断价值.方法 收集首都医科大学附属北京同仁医院病理科2002年5月至2015年1月期间,经组织学和临床资料证实的原发鼻腔鼻窦腺癌41例,其中非肠型腺癌12例,肠型腺癌10例,涎腺型肿瘤19例(腺样囊性癌12例、多形性低度恶性腺癌3例、黏液腺癌2例、腺泡细胞癌2例).采用免疫组织化学染色(En Vsion法)检测CK7、CK20、SOX10和CDX2在癌组织中的表达情况.结果 CDX2在10例原发肠型腺癌(10/10)中呈阳性表达,其中8例弥漫阳性,2例部分阳性;CK20在9例(9/10)肠型腺癌中弥漫阳性表达,1例非肠型腺癌(1/12)中部分表达,其余腺癌两者均阴性.CK7在7例肠型腺癌(7/10)中阴性表达,3例肠型腺癌(3/10)、12例非肠型腺癌(12/12)和19例涎腺型肿瘤(19/19)中呈阳性表达.正常鼻腔鼻窦黏膜上皮表达CK7,不表达CK20及CDX2.SOX10在10例肠型腺癌(10/10)和2例非肠型腺癌(2/12)中呈阴性表达,在10例非肠型腺癌(10/12)和19例涎腺型肿瘤(19/19)中呈阳性表达.CK7-、CK20+、SOX10-和CDX2+在鼻腔鼻窦肠型腺癌中表达的灵敏度分别为70.0％、90.0％、100％和100％,特异度分别为100％、96.8％、93.5％和100％.结论 CK7-、CK20+、SOX10-和CDX2+在鼻腔鼻窦肠型腺癌中表达有较高的灵敏度和特异度,可能作为鼻腔鼻窦原发肠型腺癌的可靠标志物用于诊断与鉴别诊断,且CDX2对鼻腔鼻窦原发肠型腺癌的诊断价值优于CK20、CK7和SOX10.
目的 研究細胞角蛋白(CK)7、CK20、轉錄因子SOX10和尾型同源覈轉錄因子2(CDX2)在鼻腔鼻竇原髮腺癌中的錶達及其診斷和鑒彆診斷價值.方法 收集首都醫科大學附屬北京同仁醫院病理科2002年5月至2015年1月期間,經組織學和臨床資料證實的原髮鼻腔鼻竇腺癌41例,其中非腸型腺癌12例,腸型腺癌10例,涎腺型腫瘤19例(腺樣囊性癌12例、多形性低度噁性腺癌3例、黏液腺癌2例、腺泡細胞癌2例).採用免疫組織化學染色(En Vsion法)檢測CK7、CK20、SOX10和CDX2在癌組織中的錶達情況.結果 CDX2在10例原髮腸型腺癌(10/10)中呈暘性錶達,其中8例瀰漫暘性,2例部分暘性;CK20在9例(9/10)腸型腺癌中瀰漫暘性錶達,1例非腸型腺癌(1/12)中部分錶達,其餘腺癌兩者均陰性.CK7在7例腸型腺癌(7/10)中陰性錶達,3例腸型腺癌(3/10)、12例非腸型腺癌(12/12)和19例涎腺型腫瘤(19/19)中呈暘性錶達.正常鼻腔鼻竇黏膜上皮錶達CK7,不錶達CK20及CDX2.SOX10在10例腸型腺癌(10/10)和2例非腸型腺癌(2/12)中呈陰性錶達,在10例非腸型腺癌(10/12)和19例涎腺型腫瘤(19/19)中呈暘性錶達.CK7-、CK20+、SOX10-和CDX2+在鼻腔鼻竇腸型腺癌中錶達的靈敏度分彆為70.0％、90.0％、100％和100％,特異度分彆為100％、96.8％、93.5％和100％.結論 CK7-、CK20+、SOX10-和CDX2+在鼻腔鼻竇腸型腺癌中錶達有較高的靈敏度和特異度,可能作為鼻腔鼻竇原髮腸型腺癌的可靠標誌物用于診斷與鑒彆診斷,且CDX2對鼻腔鼻竇原髮腸型腺癌的診斷價值優于CK20、CK7和SOX10.
목적 연구세포각단백(CK)7、CK20、전록인자SOX10화미형동원핵전록인자2(CDX2)재비강비두원발선암중적표체급기진단화감별진단개치.방법 수집수도의과대학부속북경동인의원병이과2002년5월지2015년1월기간,경조직학화림상자료증실적원발비강비두선암41례,기중비장형선암12례,장형선암10례,연선형종류19례(선양낭성암12례、다형성저도악성선암3례、점액선암2례、선포세포암2례).채용면역조직화학염색(En Vsion법)검측CK7、CK20、SOX10화CDX2재암조직중적표체정황.결과 CDX2재10례원발장형선암(10/10)중정양성표체,기중8례미만양성,2례부분양성;CK20재9례(9/10)장형선암중미만양성표체,1례비장형선암(1/12)중부분표체,기여선암량자균음성.CK7재7례장형선암(7/10)중음성표체,3례장형선암(3/10)、12례비장형선암(12/12)화19례연선형종류(19/19)중정양성표체.정상비강비두점막상피표체CK7,불표체CK20급CDX2.SOX10재10례장형선암(10/10)화2례비장형선암(2/12)중정음성표체,재10례비장형선암(10/12)화19례연선형종류(19/19)중정양성표체.CK7-、CK20+、SOX10-화CDX2+재비강비두장형선암중표체적령민도분별위70.0％、90.0％、100％화100％,특이도분별위100％、96.8％、93.5％화100％.결론 CK7-、CK20+、SOX10-화CDX2+재비강비두장형선암중표체유교고적령민도화특이도,가능작위비강비두원발장형선암적가고표지물용우진단여감별진단,차CDX2대비강비두원발장형선암적진단개치우우CK20、CK7화SOX10.
Objective To explore the expressions of the cytokeratin 7 (CK7),cytokeratin 20 (CK20),SOX10 and cadual type homeobox transcription factor 2 (CDX2) in primary adenocarcinoma of the sinonasal tract,and evaluate their diagnostic values.Methods A total of 41 paraffin-embeded specimens of primary adenocarcinoma of the sinonasal tract treated in Beijing Tongren Hospital of Capital Medical University were selected from May 2002 to January 2015.All cases were confirmed by histology and clinical data,including 12 cases of non-intestinal sinonasal adenocarcinomas (non-ITACs),10 cases of intestinal sinonasal adenocarcinomas (ITACs) and 19 cases of salivary gland-type adenocarcinomas (including 12 cases of adenoid cystic carcinomas,3 cases of polymorphous low grade adenocarcinomas,2 cases of mucinous adenocarcinomas and 2 cases of acinar cell carcinomas).Expressions of CK7,CK20,SOX10 and CDX2 were assessed by immunohistochemistry staining method.Results Nuclear staining for CDX2 was identified in all the ITACs,including diffuse nuclear staining in 8 cases and partial nuclear staining in 2 cases.Cytoplasmic staining for CK20 was identified in 9 cases of ITACs,and partial cytoplasmic staining was found in 1 case of non-ITACs,while CK20 was negative in all other adenocarcinomas.Seven cases of ITACs were negative for CK7,while CK7 was positive in all other adenocarcinomas.CK7,but not CDX2 and CK20,was expressed in normal sinonasal epithelium.SOX10 was negative in 10 cases of ITACs and 2 cases of non-ITACs,and positive in all other adenocarcinomas.The sensitivity of CK7-,CK20 +,SOX10-and CDX2 + in primary ITACs of the sinonasal tract were 70.0％,90.0％,100％,100％,respectively,and the specificity were 100％,96.8％,93.5％,100％,respectively.Conclusions Expressions of CK7-,CK20 +,SOX10-and CDX2 + have high sensitivity and specificity in ITACs and can be used as a reliable diagnostic marker for primary intestinal-type adenocarcinoma of the sinonasal tract.Additionally,diagnostic value of CDX2 in primary intestinal-type adenocarcinoma of the sinonasal tract is superior to CK20,CK7 and SOX10.
