中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
30期
2483-2486
,共4页
唐勤彩%罗和生%全晓静%樊菡%余光
唐勤綵%囉和生%全曉靜%樊菡%餘光
당근채%라화생%전효정%번함%여광
P物质%结肠%肌,平滑%钙通道%钾通道
P物質%結腸%肌,平滑%鈣通道%鉀通道
P물질%결장%기,평활%개통도%갑통도
Substance P%Colon%Muscle,smooth%Calcium channels%Potassium channels
目的 研究P物质(SP)对大鼠近端结肠平滑肌细胞大电导钙激活钾通道电流(IBKCa)和L型钙通道电流(ICaL)的影响,探讨其促结肠动力的作用机制.方法 选取健康雄性Wistar大鼠24只,采用RM6240生理信号采集处理系统记录SP对大鼠近端结肠平滑肌肌条收缩的影响;采用全细胞膜片钳技术检测SP对近端结肠平滑肌细胞IBKCa和ICaL的影响.结果 10-7～10-6 mol/L的SP能促进大鼠离体结肠平滑肌纵行肌肌条的自发性紧张性收缩;10-8～10-6mol/L的SP能促进环行肌肌条的自发性收缩(均P ＜0.05).SP(10-6 mol/L)减低IBKCa,在+60 mV刺激电压下,施加SP干预后,IBKCa电流密度为(11.71±1.65) pA/pF,较对照组的(14.42 ±2.89) pA/pF明显降低(P＜0.05);SP(10-mol/L)还增大ICaL,在0 mV刺激电压下,施加SP干预后,ICaL电流密度为(-5.04±0.67)pA/pF,较对照组的(-4.25±0.46)pA/pF明显增大(P＜0.01).结论 SP能够促进大鼠离体结肠平滑肌肌条的自发性收缩,且呈浓度依赖性.SP减低IBKCa,增大ICaL.可能是SP促胃肠动力的机制之一.
目的 研究P物質(SP)對大鼠近耑結腸平滑肌細胞大電導鈣激活鉀通道電流(IBKCa)和L型鈣通道電流(ICaL)的影響,探討其促結腸動力的作用機製.方法 選取健康雄性Wistar大鼠24隻,採用RM6240生理信號採集處理繫統記錄SP對大鼠近耑結腸平滑肌肌條收縮的影響;採用全細胞膜片鉗技術檢測SP對近耑結腸平滑肌細胞IBKCa和ICaL的影響.結果 10-7～10-6 mol/L的SP能促進大鼠離體結腸平滑肌縱行肌肌條的自髮性緊張性收縮;10-8～10-6mol/L的SP能促進環行肌肌條的自髮性收縮(均P ＜0.05).SP(10-6 mol/L)減低IBKCa,在+60 mV刺激電壓下,施加SP榦預後,IBKCa電流密度為(11.71±1.65) pA/pF,較對照組的(14.42 ±2.89) pA/pF明顯降低(P＜0.05);SP(10-mol/L)還增大ICaL,在0 mV刺激電壓下,施加SP榦預後,ICaL電流密度為(-5.04±0.67)pA/pF,較對照組的(-4.25±0.46)pA/pF明顯增大(P＜0.01).結論 SP能夠促進大鼠離體結腸平滑肌肌條的自髮性收縮,且呈濃度依賴性.SP減低IBKCa,增大ICaL.可能是SP促胃腸動力的機製之一.
목적 연구P물질(SP)대대서근단결장평활기세포대전도개격활갑통도전류(IBKCa)화L형개통도전류(ICaL)적영향,탐토기촉결장동력적작용궤제.방법 선취건강웅성Wistar대서24지,채용RM6240생리신호채집처리계통기록SP대대서근단결장평활기기조수축적영향;채용전세포막편겸기술검측SP대근단결장평활기세포IBKCa화ICaL적영향.결과 10-7～10-6 mol/L적SP능촉진대서리체결장평활기종행기기조적자발성긴장성수축;10-8～10-6mol/L적SP능촉진배행기기조적자발성수축(균P ＜0.05).SP(10-6 mol/L)감저IBKCa,재+60 mV자격전압하,시가SP간예후,IBKCa전류밀도위(11.71±1.65) pA/pF,교대조조적(14.42 ±2.89) pA/pF명현강저(P＜0.05);SP(10-mol/L)환증대ICaL,재0 mV자격전압하,시가SP간예후,ICaL전류밀도위(-5.04±0.67)pA/pF,교대조조적(-4.25±0.46)pA/pF명현증대(P＜0.01).결론 SP능구촉진대서리체결장평활기기조적자발성수축,차정농도의뢰성.SP감저IBKCa,증대ICaL.가능시SP촉위장동력적궤제지일.
Objective To investigate the effect of substance P(SP) on the spontaneous contractile activity of smooth muscle cells,the large-conductance calcium-activated potassium channel currents (IBKCa) and the L-type calcium channel currents (ICaL) in rat smooth muscle cells of the proximal colon.Methods A total of 24 healthy male Wista rats were used in this test.The change of smooth muscle strips spontaneous contraction of rat proximal colon after adding SP was recorded by a physiological signal stystem (RM6240).The IBKCa and ICaL were measured via the whole cell patch-clamp technique.Results The longitudinal muscle contraction was obviously increased concentration-dependently after adding different concentrations of SP (10-7-10-6 mol/L),so as the circular muscle while adding SP(10-8-10-6 mol/L) (all P ＜ 0.05).Compared with the control group,IBKCa was decreased after adding SP(10-6mol/L).Under the stimulating voltage of 60 mY,the IBKCa current density was (11.71 ± 1.65) pA/pF,which was significantly lower compared with the control group (14.42 ± 2.89) pA/pF (P ＜ 0.05).The ICaL was apparently increased.Under the stimulating voltage of 0 mY,the ICaL currents density was (-5.04 ± 0.67) pA/pF,compared with the control group (-4.25 ± 0.46) pA/pF,which was significantly increased (P ＜ 0.01).Conclusions SP can promote the spontaneous contractile activity of colon smooth muscle of rats in vitro.And SP decrease IBKCa representatively while apparently increase ICaL.That is probably one of the mechanism SP regulate the gastrointestinal motility.
