中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2015年
4期
239-245
,共7页
刘聪%王长虹%闫福林%韩金红%谷景阳%翟飞%单筱雯
劉聰%王長虹%閆福林%韓金紅%穀景暘%翟飛%單篠雯
류총%왕장홍%염복림%한금홍%곡경양%적비%단소문
抑郁症%大鼠%海马%氟西汀%慢性不可预见性刺激%丰富环境
抑鬱癥%大鼠%海馬%氟西汀%慢性不可預見性刺激%豐富環境
억욱증%대서%해마%불서정%만성불가예견성자격%봉부배경
Depressive disorder%Rats%Hippocampus%Fluoxetine%Chronic unpredictable stress%Enrich environment
目的 探讨慢性不可预见性刺激(chronic unpredictable stress,CUS)大鼠海马微管相关蛋白-2(microtubule associated protein-2,MAP-2)表达水平的变化,氟西汀联合丰富环境对CUS大鼠的作用.方法 将30只成年雄性Sprague-Dawle(SD)大鼠采用随机数字表法分为CUS组、正常组、CUS+氟西汀组、CUS+丰富环境组、CUS+氟西汀+丰富环境组,每组6只.CUS组均孤养并接受6周CUS,正常组标准环境每笼3只群养6周,剩余3组接受6周CUS并于第3周末至第6周末分别氟西汀、丰富环境、氟西汀+丰富环境干预,CUS前后及干预后以糖水消耗实验、体重测量、旷场实验对大鼠行为进行评估,以免疫组织化学法测定各组大鼠海马MAP-2表达水平.结果 (1)CUS前各组大鼠行为评估组间两两比较,糖水消耗、体重、水平运动距离、直立次数、粪便粒数差异均无统计学意义.(2)CUS后CUS组、CUS+氟西汀组、CUS+丰富环境组、CUS+氟西汀+丰富环境组较正常组糖水消耗少、体重增加少、水平运动距离小(均P＜0.05).(3)干预后正常组、CUS+氟西汀组、CUS+丰富环境组、CUS+氟西汀+丰富环境组较CUS组糖水消耗多、体重增加多、水平运动距离大(均P＜0.05);正常组[(84±3)g,(6 687±664) cm]较CUS+氟西汀组[(75±4)g,(5 859±624) cm]、CUS+丰富环境组[(77±8)g,(5 876±784) cm]体重增加多(P=0.005、0.029)、水平运动距离大(P=0.028、0.031);CUS+氟西汀+丰富环境组[(6 657±430) cm]较CUS+氟西汀组、CUS+丰富环境组水平运动距离大(P=0.033、0.037).(4)免疫组织化学显示,正常组(0.408±0.014、0.405±0.011、0.406±0.012)、CUS+氟西汀组(0.403±0.011、0.403±0.011、0.403±0.012)、CUS+丰富环境组(0.406±0.015、0.399±0.013、0.406±0.017)、CUS+氟西汀+丰富环境组(0.407±0.015、0.401±0.010、0.407±0.013)MAP-2在海马CA1、CA3和齿状回区表达水平较CUS组(0.379±0.020、0.390±0.014、0.394±0.013)增加(均P＜0.05).结论 氟西汀、丰富环境、氟西汀联合丰富环境均可能逆转CUS大鼠抑郁样行为,氟西汀联合丰富环境效果可能优于单独使用氟西汀或丰富环境;CUS大鼠海马CA1、CA3、齿状回区MAP-2表达水平可能降低,且可能被上述3种方法逆转.
目的 探討慢性不可預見性刺激(chronic unpredictable stress,CUS)大鼠海馬微管相關蛋白-2(microtubule associated protein-2,MAP-2)錶達水平的變化,氟西汀聯閤豐富環境對CUS大鼠的作用.方法 將30隻成年雄性Sprague-Dawle(SD)大鼠採用隨機數字錶法分為CUS組、正常組、CUS+氟西汀組、CUS+豐富環境組、CUS+氟西汀+豐富環境組,每組6隻.CUS組均孤養併接受6週CUS,正常組標準環境每籠3隻群養6週,剩餘3組接受6週CUS併于第3週末至第6週末分彆氟西汀、豐富環境、氟西汀+豐富環境榦預,CUS前後及榦預後以糖水消耗實驗、體重測量、曠場實驗對大鼠行為進行評估,以免疫組織化學法測定各組大鼠海馬MAP-2錶達水平.結果 (1)CUS前各組大鼠行為評估組間兩兩比較,糖水消耗、體重、水平運動距離、直立次數、糞便粒數差異均無統計學意義.(2)CUS後CUS組、CUS+氟西汀組、CUS+豐富環境組、CUS+氟西汀+豐富環境組較正常組糖水消耗少、體重增加少、水平運動距離小(均P＜0.05).(3)榦預後正常組、CUS+氟西汀組、CUS+豐富環境組、CUS+氟西汀+豐富環境組較CUS組糖水消耗多、體重增加多、水平運動距離大(均P＜0.05);正常組[(84±3)g,(6 687±664) cm]較CUS+氟西汀組[(75±4)g,(5 859±624) cm]、CUS+豐富環境組[(77±8)g,(5 876±784) cm]體重增加多(P=0.005、0.029)、水平運動距離大(P=0.028、0.031);CUS+氟西汀+豐富環境組[(6 657±430) cm]較CUS+氟西汀組、CUS+豐富環境組水平運動距離大(P=0.033、0.037).(4)免疫組織化學顯示,正常組(0.408±0.014、0.405±0.011、0.406±0.012)、CUS+氟西汀組(0.403±0.011、0.403±0.011、0.403±0.012)、CUS+豐富環境組(0.406±0.015、0.399±0.013、0.406±0.017)、CUS+氟西汀+豐富環境組(0.407±0.015、0.401±0.010、0.407±0.013)MAP-2在海馬CA1、CA3和齒狀迴區錶達水平較CUS組(0.379±0.020、0.390±0.014、0.394±0.013)增加(均P＜0.05).結論 氟西汀、豐富環境、氟西汀聯閤豐富環境均可能逆轉CUS大鼠抑鬱樣行為,氟西汀聯閤豐富環境效果可能優于單獨使用氟西汀或豐富環境;CUS大鼠海馬CA1、CA3、齒狀迴區MAP-2錶達水平可能降低,且可能被上述3種方法逆轉.
목적 탐토만성불가예견성자격(chronic unpredictable stress,CUS)대서해마미관상관단백-2(microtubule associated protein-2,MAP-2)표체수평적변화,불서정연합봉부배경대CUS대서적작용.방법 장30지성년웅성Sprague-Dawle(SD)대서채용수궤수자표법분위CUS조、정상조、CUS+불서정조、CUS+봉부배경조、CUS+불서정+봉부배경조,매조6지.CUS조균고양병접수6주CUS,정상조표준배경매롱3지군양6주,잉여3조접수6주CUS병우제3주말지제6주말분별불서정、봉부배경、불서정+봉부배경간예,CUS전후급간예후이당수소모실험、체중측량、광장실험대대서행위진행평고,이면역조직화학법측정각조대서해마MAP-2표체수평.결과 (1)CUS전각조대서행위평고조간량량비교,당수소모、체중、수평운동거리、직립차수、분편립수차이균무통계학의의.(2)CUS후CUS조、CUS+불서정조、CUS+봉부배경조、CUS+불서정+봉부배경조교정상조당수소모소、체중증가소、수평운동거리소(균P＜0.05).(3)간예후정상조、CUS+불서정조、CUS+봉부배경조、CUS+불서정+봉부배경조교CUS조당수소모다、체중증가다、수평운동거리대(균P＜0.05);정상조[(84±3)g,(6 687±664) cm]교CUS+불서정조[(75±4)g,(5 859±624) cm]、CUS+봉부배경조[(77±8)g,(5 876±784) cm]체중증가다(P=0.005、0.029)、수평운동거리대(P=0.028、0.031);CUS+불서정+봉부배경조[(6 657±430) cm]교CUS+불서정조、CUS+봉부배경조수평운동거리대(P=0.033、0.037).(4)면역조직화학현시,정상조(0.408±0.014、0.405±0.011、0.406±0.012)、CUS+불서정조(0.403±0.011、0.403±0.011、0.403±0.012)、CUS+봉부배경조(0.406±0.015、0.399±0.013、0.406±0.017)、CUS+불서정+봉부배경조(0.407±0.015、0.401±0.010、0.407±0.013)MAP-2재해마CA1、CA3화치상회구표체수평교CUS조(0.379±0.020、0.390±0.014、0.394±0.013)증가(균P＜0.05).결론 불서정、봉부배경、불서정연합봉부배경균가능역전CUS대서억욱양행위,불서정연합봉부배경효과가능우우단독사용불서정혹봉부배경;CUS대서해마CA1、CA3、치상회구MAP-2표체수평가능강저,차가능피상술3충방법역전.
Objective To discuss the change of the expression level of microtubule associated protein-2 (MAP-2) in hippocampus and the effect of fluoxetine combine with enrich environment in chronic unpredictable stress (CUS) rat.Methods Divided 30 male Sprague-Dawle (SD) rats into CUS group,normal group,CUS + fluoxetine group,CUS + enrich environment group,and CUS + fluoxetine + enrich environment group averagely and randomly according to random number table (n=6 each).Rats in CUS group were all fed lonely and received CUS for 6 weeks.Rats in normal group were fed in groups (3 rats in every cage) in standard environment for 6 weeks.Rats in CUS+fluoxetine group,CUS+enrich environment group,and CUS + fluoxetine + enrich environment group received CUS for 6 weeks and had a lavage with fluoxetine,an intervention of enrich environment,a lavage with fluoxetine + an intervention of enrich environment respectively during the last 3 weeks every day.Every rat went a behavioral assessment before and after CUS and after intervention.Behavioral assessment included sucrose water consumption test,weight measurement,and open field test (horizontal moving distance,number of vertical,number of faece).Measured the level of MAP-2 expressed in hippocampus with immunohistochemistry at last.Results (1) Behavioral assessment before CUS:there was no significant difference between CUS group,normal group,CUS + fluoxetine group,CUS + enrich environment group and CUS + fluoxetine + enrich environment group in sucrose water consumption test,weight measurement,horizontal moving distance,number of vertical and number of faece.(2) Behavioral assessment after CUS:consumption of sucrose water,gain of body weight,distance of horizontal moving of rats in CUS group,CUS + fluoxetine group,CUS + enrich environment group,and CUS+fluoxetine+enrich environment group were all less than rats in normal group (all P＜0.05).(3)Behavioral assessment after intervention:consumption of sucrose water,gain of body weight,and distance of horizontal moving of rats in normal group,CUS + fluoxetine group,CUS + enrich environment group,and CUS+fluoxetine+enrich environment group were all more than rats in CUS group (all P＜0.05).Gain of body weight (P=0.005,0.029),and distance of horizontal moving (P=0.028,0.031) of rats in normal group ((84±+3) g,(6 687±664) cm)were more than rats in CUS+fluoxetine group ((75±4) g,(5 859±624) cm) and CUS±enrich environment group ((77±8) g,(5 876±784) cm).Horizontal moving distance of rats in CUS+fluoxetine+enrich environment group ((6 657±430) cm) were longer than rats in CUS+fluoxetine group and CUS+enrich environment group (P=0.033,0.037).(4)Results of immunohistochemistry:the levels of MAP-2 expressed in CA1,CA3,dentate gyrus in hippocampus ofrats in normal group (0.408±0.014,0.405±0.011,0.406 ± 0.012),CUS + fluoxetine group (0.403 ± 0.011,0.403 ± 0.011,0.403 ± 0.012),CUS + enrich environment group (0.406±0.015,0.399±0.013,0.406±0.017),and CUS+fluoxetine+enrich environment group (0.407±0.015,0.401±0.010,0.407 ±0.013) were all higher than rats in CUS group (0.379±0.020,0.390 ± 0.014,0.394± 0.013;all P＜0.05).Conclusions Fluoxetine,enrich environment and fluoxetine combine with enrich environment all could reverse the depressive behaviors of CUS rats,the effect of fluoxetine combine with enrich environment may be superior to fluoxetine or enrich environment alone.The expression level of MAP-2 in CA1,CA3,dentate gyrus of hippocampus in CUS rats may be decreased,fluoxetine,enrich environment and fluoxetine combine with enrich environment all could reverse the decrease.
