国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
13期
1000-1003
,共4页
汪沛%徐蓓%任丽芬%张志平%辛毅娟%徐焰
汪沛%徐蓓%任麗芬%張誌平%辛毅娟%徐燄
왕패%서배%임려분%장지평%신의연%서염
白介素25%哮喘%细胞增殖%气道平滑肌细胞
白介素25%哮喘%細胞增殖%氣道平滑肌細胞
백개소25%효천%세포증식%기도평활기세포
Interleukin-25%Asthma%Cell proliferation%Airway smooth muscle cell
目的 研究表明支气管哮喘(简称哮喘)患者血清中IL-25的含量显著增加,并且与气道重塑高度相关,然而其具体机制尚未见报道.本文旨在探讨IL-25对大鼠气道平滑肌细胞增殖活性的影响及其机制.方法 原代培养大鼠气道平滑肌细胞,IL-25处理后,采用MTT法检测细胞增殖活性,并运用流式细胞术检测细胞周期.进一步采用real-time PCR法和western blot法检测细胞周期蛋白eyclinD1 mRNA和蛋白表达,探讨IL 25对气道平滑肌细胞增殖活性影响的机制.结果 MTT结果显示IL-25可呈剂量和浓度依赖性地上调原代大鼠气道平滑肌细胞的增殖活性,流式细胞术检测结果表明IL-25可促进细胞G1期向S期转变,其可能机制与IL-25上调细胞周期蛋白cyclin D1的表达有关.结论 IL-25可能是哮喘时导致气道重塑的重要因素之一.
目的 研究錶明支氣管哮喘(簡稱哮喘)患者血清中IL-25的含量顯著增加,併且與氣道重塑高度相關,然而其具體機製尚未見報道.本文旨在探討IL-25對大鼠氣道平滑肌細胞增殖活性的影響及其機製.方法 原代培養大鼠氣道平滑肌細胞,IL-25處理後,採用MTT法檢測細胞增殖活性,併運用流式細胞術檢測細胞週期.進一步採用real-time PCR法和western blot法檢測細胞週期蛋白eyclinD1 mRNA和蛋白錶達,探討IL 25對氣道平滑肌細胞增殖活性影響的機製.結果 MTT結果顯示IL-25可呈劑量和濃度依賴性地上調原代大鼠氣道平滑肌細胞的增殖活性,流式細胞術檢測結果錶明IL-25可促進細胞G1期嚮S期轉變,其可能機製與IL-25上調細胞週期蛋白cyclin D1的錶達有關.結論 IL-25可能是哮喘時導緻氣道重塑的重要因素之一.
목적 연구표명지기관효천(간칭효천)환자혈청중IL-25적함량현저증가,병차여기도중소고도상관,연이기구체궤제상미견보도.본문지재탐토IL-25대대서기도평활기세포증식활성적영향급기궤제.방법 원대배양대서기도평활기세포,IL-25처리후,채용MTT법검측세포증식활성,병운용류식세포술검측세포주기.진일보채용real-time PCR법화western blot법검측세포주기단백eyclinD1 mRNA화단백표체,탐토IL 25대기도평활기세포증식활성영향적궤제.결과 MTT결과현시IL-25가정제량화농도의뢰성지상조원대대서기도평활기세포적증식활성,류식세포술검측결과표명IL-25가촉진세포G1기향S기전변,기가능궤제여IL-25상조세포주기단백cyclin D1적표체유관.결론 IL-25가능시효천시도치기도중소적중요인소지일.
Objective The level of interleukin-25 (IL-25) in serum of patients with bronchial asthma (asthma) was reported significantly increasing,and highly correlated with airway remodeling.But the exact mechanism has not been reported.This study aims to investigate the effect of IL-25 on proliferation of airway smooth muscle cell in rats.Methods Primary rat airway smooth muscle cells were treated with IL-25.MTT assay was used to detect cell proliferation and cell cycle was detected by flow cytometry.Real-time PCR and Western blot were employed to detect the mRNA and protein expression of cyclinD1,to explore the mechanism of IL-25 on cell proliferation of airway smooth muscle.Results MTT results showed that IL-25 increased the cell proliferation of primary rat airway smooth muscle cell in a dose-and concentration-dependent manner.Flow cytometry results showed that IL-25 can promote cell G1 to S phase transition,which mechanism and IL-25 may regulate the expression of cell cycle related proteins cyclin D1.Conclusions IL-25 may be an important factor leading to airway remodeling in asthma.
