CAJ | 학술논문

目的观察非小细胞肺癌(NSCLC)中腺癌患者EGFR基因突变情况及其与临床特征的关系。方法采用RT-PCR扩增和DNA测序技术分析肺腺癌EGFR基因第19和2l号外显子突变情况，并分析其与临床因素的相关性。结果152例肺腺癌患者中有98例(64.5%)发生EGFR基因突变，其中45例(45.9%)为发生于第19号外显子的突变，54例(54.1%)为发生于第21号外显子的突变。入组患者中，女性突变率为58.2%高于男性(48.1%)，且具有统计学意义。非吸烟者突变率为41.5%，低于长期吸烟者(57.1%)。肺腺癌EGFR基因突变患者中，Ⅰ期有50例(51.0%)，Ⅱ期20例(20.4%)，Ⅲ期28例(28.6%)。结论在肺腺癌中，EGFR基因突变以Ⅰ期多见，其中以第21号外显子的突变较为常见，与年龄、性别及吸烟无明显相关性。
목적관찰비소세포폐암(NSCLC)중선암환자EGFR기인돌변정황급기여림상특정적관계。방법채용RT-PCR확증화DNA측서기술분석폐선암EGFR기인제19화2l호외현자돌변정황，병분석기여림상인소적상관성。결과152례폐선암환자중유98례(64.5%)발생EGFR기인돌변，기중45례(45.9%)위발생우제19호외현자적돌변，54례(54.1%)위발생우제21호외현자적돌변。입조환자중，녀성돌변솔위58.2%고우남성(48.1%)，차구유통계학의의。비흡연자돌변솔위41.5%，저우장기흡연자(57.1%)。폐선암EGFR기인돌변환자중，Ⅰ기유50례(51.0%)，Ⅱ기20례(20.4%)，Ⅲ기28례(28.6%)。결론재폐선암중，EGFR기인돌변이Ⅰ기다견，기중이제21호외현자적돌변교위상견，여년령、성별급흡연무명현상관성。
Objective To investigate gene mutations of the epidermal growth factor receptor (EGFR)in non-smal cel lung cancers (NSCLC)with adenocarcinoma and their relationship with clinical factors.Methods Mutations of exons 19 and 21 of the EGFR in 152 NSCLC with adenocarcinomas were detected by Real-time PCR-amplifying and gene sequencing,and the relationship between mutations and clinical characters of NSCLC with adenocarcinomas were analyzed.Results We detected 98 EGFR mutations(64.5%)in 152 cases,the majority of which was in-frame exon 45(45.9%)and 54 cases was in exon 21(54.9%).Moreover,in NSCLC patients with adenocarcinoma,mutations were significantly more frequently observed in females(58.2%)than in males(48.1%),and significantly lower in non-smokers(41.5%)than in smokers(57.1%).In adenocarcinoma patients with mutation of EGFR,we detected 50 cases in StageⅠ(51.0%),20 cases in StageII(20.4%),28 cases in StageⅢ(28.6%). Median survival of EGFR mutations patients is 23.10±1.61 months.Conclusion Our data suggest that mutation frequency of EGFR in lung cancers patients with adenocarcinomas is Stage I,and majority of which were in exon 21.