CAJ | 학술논문

目的：通过改良传统四氯化碳（ CCl4）法诱导兔肝纤维化模型，提高以肝纤维化模型为基础的相关研究的实验效率及动物模型质量。方法：选取50只健康雄性新西兰大白兔，每批25只。首批实验（传统组）将动物随机分为两组，造模组21只，皮下注射50％CCl4橄榄油溶液，每周两次；对照组4只，相同方法皮下注射纯橄榄油，注射12周，观察记录兔一般情况。造模组于0、4、8、12周行影像学及血清学生化指标检测，每次采血后随机处死7只兔（兔意外死亡时按剩余数量均分计），测门静脉压（ PVP）并取组织行HE、Masson、免疫组化染色及ISHAK评分；对照组12周后处死大白兔，其余处理同造模组。针对第1批实验中暴露的问题进行改良，将改良方案应用于第2批（改良组）实验。结果：传统造模组及改良造模组动物死亡率分别为42．9％和9．5％，造模成功率分别为57．1％和85．7％，两组比较差异均有统计学意义（ P＜0．05）。改良组兔体重、丙氨酸氨基转移酶、天冬氨酸转氨酶、白蛋白、总胆红素与对照组相比随实验进行出现差异有统计学意义的变化（ P＜0．05）；造模组12周时门静脉血流速度较对照组减慢，螺旋CT扫描显示造模组肝实质密度较对照组降低，HE、Masson及免疫组化染色可见肝实质发生明显纤维化改变并可见假小叶形成，造模组4、8、12周时ISHAK评分分别为（1．33±0．52）、（1．83±0．75）、（4．17±0．75）分。结论：通过对CCl4诱导兔肝纤维化模型的传统造模方案进行针对性改良，可在保证造模成功率的基础上降低动物死亡率，为以动物肝纤维化模型为基础的实验研究打下良好基础。
목적：통과개량전통사록화탄（ CCl4）법유도토간섬유화모형，제고이간섬유화모형위기출적상관연구적실험효솔급동물모형질량。방법：선취50지건강웅성신서란대백토，매비25지。수비실험（전통조）장동물수궤분위량조，조모조21지，피하주사50％CCl4감람유용액，매주량차；대조조4지，상동방법피하주사순감람유，주사12주，관찰기록토일반정황。조모조우0、4、8、12주행영상학급혈청학생화지표검측，매차채혈후수궤처사7지토（토의외사망시안잉여수량균분계），측문정맥압（ PVP）병취조직행HE、Masson、면역조화염색급ISHAK평분；대조조12주후처사대백토，기여처리동조모조。침대제1비실험중폭로적문제진행개량，장개량방안응용우제2비（개량조）실험。결과：전통조모조급개량조모조동물사망솔분별위42．9％화9．5％，조모성공솔분별위57．1％화85．7％，량조비교차이균유통계학의의（ P＜0．05）。개량조토체중、병안산안기전이매、천동안산전안매、백단백、총담홍소여대조조상비수실험진행출현차이유통계학의의적변화（ P＜0．05）；조모조12주시문정맥혈류속도교대조조감만，라선CT소묘현시조모조간실질밀도교대조조강저，HE、Masson급면역조화염색가견간실질발생명현섬유화개변병가견가소협형성，조모조4、8、12주시ISHAK평분분별위（1．33±0．52）、（1．83±0．75）、（4．17±0．75）분。결론：통과대CCl4유도토간섬유화모형적전통조모방안진행침대성개량，가재보증조모성공솔적기출상강저동물사망솔，위이동물간섬유화모형위기출적실험연구타하량호기출。
Objective: To increase experimental efficiency and animal model’s quality of fundamental research which based on liver fibrosis model of animal by modify the traditional process of carbon tetrachloride ( CCl4 ) <br> induced liver fibrosis of rabbit.Methods:50 healthy male new Zealand rabbits were included in this research and divided into 2 batches on average.Rabbits in first batch( traditional batch) were separated into 2 groups:modeling group(n=21) and control group(n=4).Rabbits in modeling group were injected with 50%CCl4 olive oil solution subcutaneously twice a week for 12 weeks in succession while control group were treated with the same amount of pure olive oil.Rabbits in modeling group received color Doppler ultrasound, spiral computed tomography and serum biochemical examination every 4 weeks from the beginning to 12 weeks, 7 rabbits ( remanent were divided equally when rabbits died unexpected ) were sacrificed after blood drawing every 4 weeks to measure portal venous pressure(PVP) and obtained liver tissues for biopsy and immunohistochemical(IHC) staining as well as staging according the ISHAK grading system; Control group were treated with the same way with modeling group and sacrificed at 12 weeks.Modify disadvantages exposed during experiment of first batch and apply revised scheme to the second batch( modified batch) .Results:Mortality of traditional patch was 42.9%and significantly higher than modified batch of 9.5%( P<0.05 ) and the success rate of traditional patch ( 57.1%) was obviously lower than that of modified batch ( 85.7%) , too.With progression of modeling process, ALT, AST, ALB, TP of modeling group underwent significant changes compare to control group ( P<0.05 ); color doppler flow imaging showed the speed of portal venous blood flow of modeling group was obviously slower and CT values were significantly reduced comparing to control group;HE, Masson and IHC staining showed explicit liver fibrosis and pseudolobule could be seen, ISHAK score rised over time(1.33 ±0.52,1.83 ±0.75,4.17 ±0.75 of modified modeling group at 4,8,12 week respectively).Conclusion:Continuous injection of CCl4 can induce explicit liver fibrosis of rabbit.By adjust modeling process of modeling process we can reduce mortality of animals drastically and guarantee the success rate at the same time and provide useful information for research based on liver fibrosis animal models.