CAJ | 학술논문

目的：探讨心肺体外循环(CPB)对大鼠长期空间认知功能和脑内炎性反应的影响。方法雄性成年SD大鼠随机分为对照组、假手术组和CPB组，每组6只。在CPB术后20周，采用8-臂迷宫实验测定大鼠的空间认知功能，应用免疫组化学检测海马区微胶质细胞的活化情况。结果与对照组和假手术组比较，CPB组呈现明显的学习和记忆能力损害。CPB组训练所需的次数较其他两组显著增加；在记忆测试中，间隔3 h和6 h后，CPB组记忆准确率明显低于其他两组(P<0.05)。免疫组化学检测示CPB组海马区有活化的微胶质细胞。结论 CPB可引起长期认知功能损害，这些损害可能与CPB引起海马区微胶质细胞活化有关。
목적：탐토심폐체외순배(CPB)대대서장기공간인지공능화뇌내염성반응적영향。방법웅성성년SD대서수궤분위대조조、가수술조화CPB조，매조6지。재CPB술후20주，채용8-비미궁실험측정대서적공간인지공능，응용면역조화학검측해마구미효질세포적활화정황。결과여대조조화가수술조비교，CPB조정현명현적학습화기억능력손해。CPB조훈련소수적차수교기타량조현저증가；재기억측시중，간격3 h화6 h후，CPB조기억준학솔명현저우기타량조(P<0.05)。면역조화학검측시CPB조해마구유활화적미효질세포。결론 CPB가인기장기인지공능손해，저사손해가능여CPB인기해마구미효질세포활화유관。
Aim To observe the effect of experimental cardiopulmonary bypass (CPB) on persistent cognitive impairment and cerebral inlfammatory reaction in rats. Methods The adult male Sprague-Dawley rats were randomly assigned to control, sham, and CPB groups. Twenty weeks later, cognitive function was tested with 8-arm maze. Immunohistochemical study was used to detect the activation of microglia. Results The ability of learning and memory was impaired in CPB rats compared with that of the sham and the control rats. The numbers of attempts required to achieve criterion performance at the 15 minute interval were greater in the CPB rats. Statistical signiifcance was achieved in tests of memory impairment at the intervals of both 3 and 6 hours in the CPB rats. Compared with other two groups, microglia cells were signiifcantly activated in hippocampus of the CPB rats. Conclusion CPB would cause long-term cognitive deifcits in rats, which might be induced by microglial activation in hippocampus after CPB.