CAJ | 학술논문

目的：了解山西省感染性腹泻的细菌病原学分布特征及耐药性，以指导其临床用药。方法采集798例感染性腹泻患者粪便标本，分离可疑病原菌，用聚合酶链技术（PCR）、生化试验、血清凝集试验等方法鉴定可疑病原菌，分析细菌病原学分布特征；采用纸片扩增法，对分离到的病原菌进行药敏试验。结果798份标本分离各种肠道致病菌81株，阳性检出率10．15％。其中，致泻性大肠埃希菌检出最多，为46.91％（38／81），其次是志贺菌、沙门菌、气单胞菌等；对上述菌株进行纸片法药敏试验，大部分菌株对头孢西丁、头孢噻肟及环丙沙星的耐药性较低，而对四环素、萘啶酸的耐药性较高。结论山西省感染性腹泻的主要致病菌是致泻性大肠埃希菌、志贺菌、沙门菌和气单胞菌，应加强对这些菌的耐药性监测，为临床抗感染治疗提供可靠依据。
목적：료해산서성감염성복사적세균병원학분포특정급내약성，이지도기림상용약。방법채집798례감염성복사환자분편표본，분리가의병원균，용취합매련기술（PCR）、생화시험、혈청응집시험등방법감정가의병원균，분석세균병원학분포특정；채용지편확증법，대분리도적병원균진행약민시험。결과798빈표본분리각충장도치병균81주，양성검출솔10．15％。기중，치사성대장애희균검출최다，위46.91％（38／81），기차시지하균、사문균、기단포균등；대상술균주진행지편법약민시험，대부분균주대두포서정、두포새우급배병사성적내약성교저，이대사배소、내정산적내약성교고。결론산서성감염성복사적주요치병균시치사성대장애희균、지하균、사문균화기단포균，응가강대저사균적내약성감측，위림상항감염치료제공가고의거。
Objective To investigate the distribution and antibiotic resistance of the enteric pathogens in Shanxi province to guide the choice of antibacterials.Methods 798 cases of fecal samples from patients with diarrhea were treated in the outpatient and inpatient.The suspected pathogen was separated then PCR,biochemical test,serum agglutination tests were used for identification of suspected pathogen,and bacterial pathogens constitution and patho-genic characteristics were analyzed.Antimicrobial susceptibility testing was conducted by diskdiffusion method for the suspected pathogen with 6 antimicrobial agents.Results 81 strains isolated from 798 specimens were positive with 10.15% for pathogen detection.Diarrheagenic Escherichia coli was the first frequently pathogen,accounting for 46.91%,followed by Shigella,Salmonella and Aeromonas.Drug sensitivity monitoring showed that the most of the 81 strains had lower level of resistance to cefoitin,cefotaxime and ciorofloxacin,and higher level of resistance to tetra-cycline and nalidixic aid.Conclusion Diarrheagenic Escherichia coli,Shigella,Salmonella and Aeromonas are the major bacterial diarrheal pathogens in the hospital.Surveillance of antimicrobial resistance in these bacteria should be strengthened to provide reliable evidence for clinical anti infection treatment.