CAJ | 학술논문

目的：探讨非黄嘌呤类腺苷阻断剂 SLV320对慢性心衰（CHF）动物实验模型心室重构及肾素-血管紧张素-醛固酮系统（RAAS）的影响。方法将40只普通级成年雄性新西兰兔予以阿霉素静脉注射建立CHF 模型，再按照随机原则分为：高剂量组，予以静脉注射 SLV320剂量为10．0μg·kg －1·d －1；中剂量组，剂量为5．0μg·kg －1·d －1；低剂量组，剂量为2．5μg·kg －1·d －1；呋塞米组，予以灌服呋塞米2．0 mg·kg －1· d －1。每组均为10只，连续用药1周。检测并比较实验前后四组的血浆肾素（PRA）、血管紧张素Ⅱ（AngⅡ）、醛固酮（ALD）、B 型钠尿肽（BNP）水平；检测并比较实验前后四组左室收缩末内径（LVESD）、左室舒张末内径（LVEDD）、左心室后壁厚度（LVPW）、左心射血分数（LVEF）、左室缩短分数（LVFS）、E ／A 等心室功能指标；准确称重左心室及右心室，计算并比较四组左心室重量指数（LVWI）和右心室重量指数（RVWI）。结果实验前四组血浆 PRA、AngⅡ、ALD、BNP 水平差异无统计学意义（P ＞0．05），实验后四组各指标水平顺序为：高剂量组＜中剂量组＜低剂量组＜呋塞米组（均 P ＜0．05）。实验前四组各心室功能指标差异无统计学意义（P ＞0．05），实验后 LVEF 及 LVFS 指标顺序为：高剂量组＞中剂量组＞低剂量组＞呋塞米组（均P ＜0．05），而LVESD、LVEDD、LVPW、E ／A 指标顺序为：高剂量组＜中剂量组＜低剂量组＜呋塞米组（均 P ＜0.05）。治疗后 LVWI 及 RVWI 顺序为：高剂量组＜中剂量组＜低剂量组＜呋塞米组（均 P ＜0．05）。结论SLV320能改善 CHF 的心室重塑及 RASS 系统，且具有剂量依赖性。
목적：탐토비황표령류선감조단제 SLV320대만성심쇠（CHF）동물실험모형심실중구급신소-혈관긴장소-철고동계통（RAAS）적영향。방법장40지보통급성년웅성신서란토여이아매소정맥주사건립CHF 모형，재안조수궤원칙분위：고제량조，여이정맥주사 SLV320제량위10．0μg·kg －1·d －1；중제량조，제량위5．0μg·kg －1·d －1；저제량조，제량위2．5μg·kg －1·d －1；부새미조，여이관복부새미2．0 mg·kg －1· d －1。매조균위10지，련속용약1주。검측병비교실험전후사조적혈장신소（PRA）、혈관긴장소Ⅱ（AngⅡ）、철고동（ALD）、B 형납뇨태（BNP）수평；검측병비교실험전후사조좌실수축말내경（LVESD）、좌실서장말내경（LVEDD）、좌심실후벽후도（LVPW）、좌심사혈분수（LVEF）、좌실축단분수（LVFS）、E ／A 등심실공능지표；준학칭중좌심실급우심실，계산병비교사조좌심실중량지수（LVWI）화우심실중량지수（RVWI）。결과실험전사조혈장 PRA、AngⅡ、ALD、BNP 수평차이무통계학의의（P ＞0．05），실험후사조각지표수평순서위：고제량조＜중제량조＜저제량조＜부새미조（균 P ＜0．05）。실험전사조각심실공능지표차이무통계학의의（P ＞0．05），실험후 LVEF 급 LVFS 지표순서위：고제량조＞중제량조＞저제량조＞부새미조（균P ＜0．05），이LVESD、LVEDD、LVPW、E ／A 지표순서위：고제량조＜중제량조＜저제량조＜부새미조（균 P ＜0.05）。치료후 LVWI 급 RVWI 순서위：고제량조＜중제량조＜저제량조＜부새미조（균 P ＜0．05）。결론SLV320능개선 CHF 적심실중소급 RASS 계통，차구유제량의뢰성。
Objective To analyze the effect of non -xanthine adenosine receptor antagonist (SLV320)on the ventricular remodeling and renin -angiotensin -aldosterone system (RAAS)in animal experimental models of chronic heart failure (CHF).Methods The 40 healthy male New Zealand rabbits were received adriamycin by intra-venous injection to establishing the experimental animal models and were randomly divided into 4 groups,which were high -dosage group (injected with SLV320,10.0μg·kg -1 ·d -1 ),medium -dosage group (injected with SLV320, 5.0μg·kg -1 ·d -1 ),low -dosage group (injected with SLV320,2.5μg·kg -1 ·d -1 )and furosemide group (fed with furosemide,2.0mg·kg -1 ·d -1 ).Each group had 10 rabbits and continuous treated for one week.The indexes of plasma renin activity (PRA),angiotensinⅡ (AngⅡ),aldosterone (ALD)and beta ntriuretic peptide (BNP)were detected at pre -and post -treatment,and compared among 4 groups.The indexes of left ventricular end -systolic dimension (LVESD),left ventricular end -diastolic dimension (LVEDD),left ventricular posterior wall (LVPW), left ventricle ejection fraction (LVEF),left ventricular fractional shortening (LVFS)and E /A at pre -and post -treatment were detected by echocardiography and compared among 4 groups.The wet weigh of the left and right ventri-cle were weigh accurately.And the indexes of left ventricle weight index (LVWI)and the body weight index (BWI) were calculated and compared among 4 groups.Results The plasma levels of PRA,AngⅡ,ALD and BNP were no different among 4 groups before study (all P >0.05).The sequence of 4 groups on the plasma levels of RAAS indexes was high -dosage group <medium -dosage group <low -dosage group <furosemide group (all P <0.05).The ven-tricular function indexes were no different among 4 groups before study (all P >0.05).The sequence of 4 groups on the levels of LVEF and LVFS was high -dosage group >medium -dosage group >low -dosage group >furosemide group,and the sequences of LVESD,LVEDD,LVPW and E /A were high -dosage group <medium -dosage group <low -dosage group <furosemide group (all P <0.05).The sequences of 4 groups on the LVWI and RVWI were high-dosage group <medium -dosage group <low -dosage group <furosemide group (all P <0.05 ).Conclusion SLV320 can regulate the ventricular remodeling and RAAS in animal model of CHF and this effect was dose dependent.