CAJ | 학술논문

以云南玉龙雪山自然保护区内不同海拔梯度（ A1：2750 m， A2：2900 m， A3：3050 m， A4：3200 m， A5：3350 m， A6：3500 m）的川滇高山栎为研究对象，通过分析其主要生理生化指标变化，探讨了该树种适应垂直气候环境变化的生理机制。结果表明，随着海拔的升高，川滇高山栎叶片各生理指标呈非线性变化。在较低海拔点（2750 m）及较高海拔点（3500 m）的叶绿素含量均显著降低，而在中间海拔点（3200 m）的叶绿素含量增加；在海拔3050 m处，膜脂过氧化产物MDA含量、脯胺酸含量、可溶性糖含量、抗氧化酶（ SOD、CAT、 APX）活性等指标均处于最低水平，而当海拔超过或低于3050 m时，其值都有所增加。海拔3050 m是川滇高山栎的最适生长区域，光照充足，叶绿素含量较多，生长较快，膜脂过氧化程度轻；在低海拔区域，由于光照不足或人为破坏，导致其生长较缓慢；在高海拔区域，虽然该物种能通过抗氧化酶活性的增加和可溶性细胞代谢物质的调节形成一定的生态适应，但随海拔的进一步增加（3500 m），由于低温、大气稀薄或强的紫外辐射等不利环境的加剧，植株体内的抵御能力有限，亦限制了川滇高山栎的生长发育。该研究结果可为西南地区川滇高山栎天然林的保护提供一定的理论支持。
이운남옥룡설산자연보호구내불동해발제도（ A1：2750 m， A2：2900 m， A3：3050 m， A4：3200 m， A5：3350 m， A6：3500 m）적천전고산력위연구대상，통과분석기주요생리생화지표변화，탐토료해수충괄응수직기후배경변화적생리궤제。결과표명，수착해발적승고，천전고산력협편각생리지표정비선성변화。재교저해발점（2750 m）급교고해발점（3500 m）적협록소함량균현저강저，이재중간해발점（3200 m）적협록소함량증가；재해발3050 m처，막지과양화산물MDA함량、포알산함량、가용성당함량、항양화매（ SOD、CAT、 APX）활성등지표균처우최저수평，이당해발초과혹저우3050 m시，기치도유소증가。해발3050 m시천전고산력적최괄생장구역，광조충족，협록소함량교다，생장교쾌，막지과양화정도경；재저해발구역，유우광조불족혹인위파배，도치기생장교완만；재고해발구역，수연해물충능통과항양화매활성적증가화가용성세포대사물질적조절형성일정적생태괄응，단수해발적진일보증가（3500 m），유우저온、대기희박혹강적자외복사등불리배경적가극，식주체내적저어능력유한，역한제료천전고산력적생장발육。해연구결과가위서남지구천전고산력천연림적보호제공일정적이론지지。
Taking Quercus aquifolioides, which grown at different altitude gradient ( A1: 2 750 m, A2: 2 900 m, A3: 3 050 m, A4: 3 200 m, A5: 3 350 m, A6: 3 500 m) of Nature Reserve in Yulong Snow Mountain as the main materials, the main physiological and biochemical indexes were studied and the physiological mechanisms of Quercus aquifolioides responses to changes of vertical climate were analyzed .The results showed that with the in-crease of altitude, there was nonlinear variation in the physiological indicators in leaves of Quercus aquifolioides. The chlorophyll contents were significantly lower at low (2 750 m) and high altitude (3 500 m), while increased at the middle altitude (3 200 m) .In addition, it was not only the content of membrane lipid peroxidation products (MDA), proline content and soluble sugar content, but also the activities of antioxidant enzyme (SOD, CAT and APX) reached to the lowest level at the 3 050 m altitude, while the values of these indicators were increased at oth-ers altitude.In conclusion, the 3 050 m altitude was an optimum zone for the growth of Quercus aquifolioides as a re-sult of sufficient sunlight , more content of chlorophyll , faster growth and lower level of membrane lipid peroxidation;At the low altitude, the lack of sunlight or man-made destruction resulted the slower growth of Quercus aquifolioides;At the high altitude, although the Quercus aquifolioides could form certain ecological adaptation by increasing the ac-tivities of antioxidant enzymes and regulating the soluble cell metabolism substance , the growth and development of Quercus aquifolioides were limited with the further increase of altitude ( reached to 3 500 m)for the seriously adverse environment, such asthe low temperature , thin atmosphere and strong ultraviolet radiation .The research could pro-vide certain theoretical support to protect the natural Quercus aquifolioides forest of Southwest China .