CAJ | 학술논문

通过对金黄色葡萄球菌免疫磁珠添加量、免疫磁珠与菌液最佳反应时间、免疫磁珠分离金黄色葡萄球菌的敏感性试验、特异性试验的研究，确定了金黄色葡萄球菌抗体添加量50μg/mL、免疫磁珠添加量100μL，免疫磁珠与菌液最佳作用时间为30 min，免疫磁珠法检测灵敏度底限为10-9，相应的细菌浓度为14 cfu/mL 。本试验制备的免疫磁珠灵敏性强，特异性高，大大节省了检测时间和费用。因此研究食品中金黄色葡萄球菌快速、准确检测方法以预防和控制食品安全事件的发生，具有重要的现实意义。
통과대금황색포도구균면역자주첨가량、면역자주여균액최가반응시간、면역자주분리금황색포도구균적민감성시험、특이성시험적연구，학정료금황색포도구균항체첨가량50μg/mL、면역자주첨가량100μL，면역자주여균액최가작용시간위30 min，면역자주법검측령민도저한위10-9，상응적세균농도위14 cfu/mL 。본시험제비적면역자주령민성강，특이성고，대대절성료검측시간화비용。인차연구식품중금황색포도구균쾌속、준학검측방법이예방화공제식품안전사건적발생，구유중요적현실의의。
According to the research of the addition amount of Staphylococcus aureus immune magnetic beads(IMB), the optimal reaction time of magnetic beads and bacteria ,and the sensitivity and specificity tests of IMB Staphylococcus aureus,it was showed that the best addition amount of Staphylococcus aureus antibody is 50μg/mL,and IMB is 100μL. The best reaction time of IMB and bacterium fluid is 30 min,and the detection sensitivity limit of magnetic beads is 10- 9,correspondingly the bacteria concentration is 14 cfu/mL. The immune magnetic beads is of strong sensitivity and high specificity,and can greatly reduce testing time and cost. Therefore,it is more and more important to establish a rapid and efficient detection of Staphylococcus aureus method,to prevent and control the safety incidents of food.